Nei Hplc ichishandiswa pakuongororwa kwezvinodhaka?

Yakakwirira-perform Flound chromatography (HPLC) yave iri yekuongorora yekuongorora muindasitiri yemishonga uye inoshandiswa zvakanyanya nekuongororwa kwezvinodhaka nekuda kwekubudirira kwayo, kunzwa, uye kunowedzera. Chinyorwa ichi chinoongorora kuti nei HPLC ndiyo nzira yavanofarira yekuongorora zvinodhaka, zvakanakira, zvikumbiro, uye basa racho rinotora mukuchengetedza zvinodhaka uye kushanda kwezvinodhaka.

HPLC rudzi rwemvura chromatography iyo inoshandisa yakanyanya kudzvinyirira kusimbisa solvent kuburikidza nekhoramu yakazadzwa neyekutamba zvinhu. Maitiro acho anoparadzanisa zvikamu zvemusanganiswa zvichienderana nekudyidzana kwavo neiyo stationary uye nhare mbozhanhare. Izvo zvikamu zvinobva zvaonekwa sezvavanoshuwira kubva mukoramu, kubvumira kuongororwa kwakakodzera uye kuwanda.

Unoda kuziva zvakawanda nezveHPLC Sampula, ndapota tarisa chinyorwa ichi: HPLC yemuenzaniso yekugadzirira mhinduro dzemhedzisiro yakanaka

Zvakakosha Zvakanakira HPLC mukuongororwa kwezvinodhaka

Kunzwa kwepamusoro uye kusarudza: HPLC inokwanisa kuona zvakanyanyisa kuderera zvakaderera zvenguva pfupi, kazhinji pasi kuNanograp kana mapikicha emazinga. Uku kunzwa kwakakosha mukuongororwa kwezvinodhaka nekuti kunyange trace huwandu hwekusvibiswa hunogona kuve nehunyanzvi hwekuchengetedza uye kushanda kwemushonga. Iko kugona kwekusarudza kwakaparadzanisa makomputa nemaitiro akafanana anowedzera kubatsira kwayo mukuongorora zvakaoma mikana.

Kubudirira uye kumhanya: HPLC ine pfupi yekuongorora nguva pane tsika yechinyakare. Uchishandisa pombi yakakwirira-yekumanikidza inobvumidza kukurumidza sol sol sol Flow rate, zvichikonzera munguva pfupi kumhanya nguva pasina kupa kuipira kugadzirisa. Kubudirira uku kunonyanya kubatsira munzvimbo dzakakwirira-dzekupfeka uko huwandu hukuru hwe samples dzinoda kuongororwa nekukurumidza.

Kuedza: HPLC kunogona kuongorora huwandu hwakawanda hwemakomputa, kusanganisira madiki madiki mamorekuru, mapepeti, mapuroteni, uye mamwe ma biobolecules. Kuita kwayo kunosanganisira kune akasiyana siyana emhando yemhando yemhando, yakadai seyakaomeswa, yepakati, uye yakapedza zvigadzirwa. Dzakasiyana-siyana dzekoramu dzemakemikari uye nzira dzekucherechedzwa dzinogona kushandiswa kugadzirisa kuongororwa kune izvo zvinodikanwa.

Kugadzikana uye kubereka: HPLC Systems yakagadzirwa nekutarisa pane kugadzikana, kuvabvumira kuti vashande nguva nenguva kwenguva yakareba. Kubereka uku kwakakosha mune zvemhando yepamusoro nzvimbo dzekudzora, uko mitemo inoda yakavimbika mhedzisiro pamusoro pezvibereko zvakawanda.

Kuzvidzora kutevedzera HPLC ndiyo nzira inozivikanwa yekudzora mhando uye vimbiso panguva yekugadzira zvinodhaka. Kugona kwayo kupa zvakadzama mitezo yakasvibiswa uye kusimbisa kuzivikanwa kweiyo inoshanda mishonga mishonga (APIS) inoita kuti ikakosha kuti isangane nemitambo yekutonga.

Zvikumbiro zveHPLC mukuongororwa kwechechi

Mhando Yekutonga (QC): MuMishonga Kugadzirwa Kugadzira, HPLC inoshandiswa mune zvemhando yepamusoro maitiro ekutarisa kuzivikanwa, kuchena, simba, uye kusimbisa mushonga. Inobatsira kuona kusachena uko kunogona kukanganisa kuchengetedza kana kushanda kwechigadzirwa. Semuenzaniso, kugadzikana kwekutarisa uchishandisa HPLC kunogona kuratidza zvigadzirwa zvekushongedza izvo zvinogona kuumba nekufamba kwenguva mamiriro akasiyana ekuchengetedza.

Budiriro yezvinodhaka: Munguva yekuvandudza zvinodhaka, HPLC inoita basa rakakosha mukuratidzira mishonga nyowani. Iyo inobatsira vatsvagiri vanonzwisisa zvimiro zvemakemikari senge solubility uye kugadzikana, vachiri kupa ruzivo rwekuita metabolic kuburikidza nePermacokinetic zvidzidzo.

Pharmacokinetics: Hplc inoshandiswa zvakanyanya muPharmacokinetic zvidzidzo kuti asayedze kuti zvinodhaka zvakadyarwa, zvakagoverwa, metabolized, uye zvakaburitswa mumuviri. Nekunyora fungidziro yezvinodhaka mumanyoro emvura, senge ropa kana urini, vaongorori vanogona kuongorora maitiro ekuratidzira uye kuchengeteka.

Bioanalytical application: MuBioanalytical Zvidzidzo, HPLC inobatsira kuyera kutarisisa kwezvinodhaka muBiological matrices. Ichi chishandiso chakakosha pakuona zvakakodzera dhizaini uye kuongorora mhinduro dzemurwere panguva yekiriniki miedzo.

Kuongororwa kusereka: Kuziva uye kufungidzira kusachena kwakakosha kuti uone kusimbisa hunhu. HPLC inopa yakadzama isina kukodzera kuongororwa kuti ibatsire vagadziri vanonzwisisa njodzi dzinogona kunge dzakabatana nezvisikwa kana zvigadzirwa zvinosvibisa.

Basa reHplc mukuita kuti uve nechokwadi chekuchengetedza zvinodhaka

Indasitiri yechechi yechechi inoenderana nemirairo yakasimba yakagadzirirwa kuchengetedza hutano hweveruzhinji. HPLC inobatsira zvakanyanya kune ichi chinangwa ne:

Kusimbisa chitupa chezvinodhaka: Kuverengera iyo chaiyo inoshanda inoshanda iripo pane yakatarwa kusarudzika.

Kuona zvinosvibisa: Kuzivisa chero chinhu chinokuvadza chinogona kuisa njodzi yekuchengetedza.

Kuongorora kugadzikana: Kuongorora kusvibiswa kweinodhaka nekufamba kwemamiriro ezvinhu akasiyana siyana kuti uone zvakakodzera zvibodzwa.

Kutsigira Kuteerana: Kupa data inodiwa kuti adzorere kutumira uye fungidziro.

Unoda kuziva zvakawanda nezvezvinoshandiswa zve chromatography vials, ndapota tarisa chinyorwa ichi:15 zvikumbiro zve chromatography vials muminda dzakasiyana

Zvinonetsa uye kufunga

Nepo Hplc inopa zvakawanda zvakanakira, kushandiswa kwayo kunounzawo matambudziko akati wandei:

Kuita kuoma kwekuvandudza nzira: Kukudziridza nzira dzekushanda dzeHPLC kunoda hunyanzvi mune nhungamiro dze chromatography pamwe nekunzwisisa kwakanzwisisika kuongororwa.

Zvekare Zvakaringana: Iyo yekutanga investment mumidziyo yeHPLC inogona kuve inokanganisika, kuwedzera kumitengo inoenderera mberi yakabatana nekugadzirisa, zvinokatyamadza (i.e., columns), uye solumns.

Muenzaniso Unogadzirira Zvido: Kugadzirira kwakakodzera kwakakosha kunokosha kuti uwane migumisiro yakarurama; Kugadzirira kusingakwanisi kunogona kukonzera kusvibiswa kana kurasikirwa kwekuongorora.

Yakakura Performance Liquid chromatography yave chishandiso chakakosha mukuongororwa kwemishonga pamwe nekunzwa kwayo kusingafadzi Zvekushandisa zvaro zvinobva kune zvemhando yepamusoro pakugadzira kune yavo yakaoma basa mukuvandudzwa kwezvinodhaka uye bioanalytical kutsvakira. Sezvo indasitiri yemishonga inoenderera mberi nekukura uye kudiwa kwekukurumidza, kunyatsoongorora HPLC kunongowedzera - kunoita kuti mishonga yakachengeteka, inoshanda, uye inosangana nemhando dzinoshanda zvisati zvasvika vatengi.

Unoda kuziva zvakawanda nezve chromatography vials yekuongorora kwemvura, ndapota tarisa chinyorwa ichi:Chromatography vials yemvura yekuongorora