Mar. 15th, 2024
Nhanganyaya
Chromatography, dombo rekonagnation muongororo yemakemikari, inofambisa kupatsanurwa uye kuwanda kwezvikamu mukati memusanganiswa wakaoma. Pasinei nekunyorera kwayo kwakapararira, chromatographic vanoongorora nezvekupindirana kwemashure, izvo zvinogona kukanganisa chokwadi uye kunyatsojeka kwemhedzisiro. Imwe yakakosha sosi yekuvhiringidzika kwemashure inobva kune chromatography vials, iyo inogona kuunza zvinongedzo uye zvigadzirwa mumuenzaniso wemuenzaniso matrix. Munyaya ino, isu tinodzungudza mune zvinangwa zvekupindirana kwekutanga kubvachromatography vials, kuongorora zvinokonzeresa zvakasiyana-siyana uye kutaura zvinobudirira maitiro ekudyira.
Kunzwisisa kwekusimbirira kumashure
Vakasara vanosvibisa
Chromatography vials inogona kuchengetedza zvakasvibiswa zvakasara kubva pakugadzira maitiro kana pekutanga kushandiswa. Izvi zvinosvibisa zvinogona kusanganisa huwandu hwezvinhu, kusanganisira lubricants, mol musunungurwe vamiriri, kana polymer anowedzera. Kana izvi zvinosvibisa leach mumuenzaniso, ivo vanounza ruzha rweseline uye vanokanganisa nekuonekwa uye kuwanda kwekuongororwa kwekuongorora. Kuvapo kwekuzvisvibisa kwakadaro kunogona kutungamira kumapepa enhema, kuderera chiratidzo-kune-ruzha ratios, uye zvakarongeka chromatographic resution.
Inobviswa uye inokara
Mamwe Midziyo Midziyo, kunyanya mapurasitiki, iva nehuwandu hwekuburitsa zvinowanikwa zvinowanikwa kana zvichiratidzwa zvine solimes kana samples. Izvi zvinobuda zvinogona kubva kune vial matrix kana vanowedzera vanoshandiswa mukukara kwayo. Pasingaregi mumuenzaniso, aya makomputa anogona kusvibisa maitiro ekuongorora, zvichikonzera kusimudzwa kwemashure ekukwira uye kukanganiswa neye aumbeate Peaks. Kuvapo kwekutora kunoisa dambudziko rakakosha mukuongorora kwakasimba, kunyanya mumisheni mishonga uye ezvekudya maindasitiri anoonekwa-mwero.
Survention Adsorption
Chromatography vialMafuro anogona kuratidzira adsorptive zvivakwa, kupi ongororo kana zvikamu zveMuenzaniso zvine chekuita nemadziro emidziyo. Ichi chiitiko chinonyatsotaurwa mugirazi mapoka egirazi, apo silanol mapoka pamusoro anogona kutaurirana ne polar kana kubhadharisa ongororo. Kushambadzira kwepasi kunotungamira kumienzaniso, pelak inokura, uye yakashandura nguva nenguva, nekudaro ichidzivirira chromatographic kududzira uye kuderedza kuongororwa kwekunzwa.
Mitemo mitambo
Kusarudzwa kweVial
Kusarudza vials yakavakwa kubva kune zvigadzirwa zvine mashoma ekuvhiringidzika kukanganiswa kwakakosha kwekudzora chromatographic. Borosilrate girazi vigial vials, anozivikanwa nekukasira uye akadzika madhiri mbopa, sarudzo inodiwa yezvakawanda zvekuongorora. Nenzira imwe, vials yakanangana neInert polysers, yakadai sePolytetraFluorohone (PTFE) kana Silicone, ipa yekudzikisira kuendesa uye nekukasira kuchinjisa chromatographic.
Pre-mamiriro
Asati uma sumo, vials vanofanirwa kunge vari kurongedza pre-mamiriro ekubvisa zvakasara zvakasvibiswa uye kudzikisira kukanganiswa kwekutanga. Kuita uku kazhinji kunosanganisira kufambisa vials nematombo akakodzera, akadai se methanol kana mvura, ichiteverwa nekuomesa pasi pemamiriro ezvinhu. Nekubvisa zvingangoitika zvekusvibiswa, pre-mamiriro anovimbisa kugadzikana kwemasaini uye anowedzera kubereka kwe chromatographic zviyero.
Mhando yekudzora
Kuita zvinhu zvakasimba kudzora matanho akakosha kuti uve nechokwadi chekuvimbika kwe chromatography vials uye kudzikisira kukanganiswa kwekupindirana. Laboratories inofanirwa kuitisa kuongorora kwekuongorora kweiyo vial kuchena, kutarisa kwekuremara kwekuona, chidimbu chechinhu, kana nzvimbo inosara. Vials inofanira kuchengetwa munzvimbo dzakachena uye dzakadzorwa dzekudzivirira kusvibiswa pakati pekushandiswa, paine chero vakurumbira zvakarererwa zvakaraswa kudzivirira zvikanganiso zvekuongorora.
Sampuli inobata
Kudzidzira maitiro akakodzera ekubata maitiro anoshanda mukudzivirira kupindira-kwakanangana ne chromatographic kuongorora. Vanoongorora vanofanira kungwarira kuti vaderedze zviyero zvekuratidzira, vachidzivisa kuvanzika zvakanyanya uye vachiratidzira chisimbiso chakakodzera kudzivirira kubuda kwemuenzaniso kana kusvibiswa. Pamusoro pezvo, kushandisa sampuli yekuenzanisa kana centrifugation inogona kubvisa zvinhu zvechikamu uye kuderedza njodzi yemuenzaniso wekutakura, nekudaro kuwedzeredza kurongeka uye precisic of chromatographic.
Nzira yekufunga
Optimizing chromatographicares inotora nzvimbo inokonzeresa mukudzora kumashure kwekuvhiringidza uye kuisimudzira ongororo yekuongorora. Vanoongorora vanofanira kuongorora nzira yekunyorwa, kusanganisira nhare mbozha, chemistry mbozha, uye ma parmetres ekushandisa, kuti azivise kupatsanurwa kupatsanurana uye chiratidzo-kune-ruzha ratios. Akanaka-tuning chromatographic emamiriro ezvinhu anogona kuderedza mhedzisiro yemashure migariro, achivandudza masimba ekugadzirisa, uye nekuvimbika kwezviyero zvehuwandu.
Mhedziso
Kukanganisika kwekutanga kubvachromatography vialsinoisa dambudziko rinotyisa mukuzadzisa chaiyo uye yakavimbika ongororo mhedzisiro. Nekunzwisisa unonzwisisa masosi ekuvhiringidza uye kushandisa maitiro ekupindirana maitiro, mabara anogona kuderedza kukanganiswa kweiyo vial-zvinoenderana ne chromatographic kuongorora. Kuburikidza nesarudzo dzesarudzo, uye nzira yemhando yepamusoro yekudzora, uye nzira yekunyora, kuongorora kunogona kuwedzera kuyera kwe chromatophi, nekukasira kutsvagisa kwetsananguro, yekudzora kutevedzera, uye maindasitiri ekushandisa.
Vhura mhinduro dzepamusoro makumi mashanu nezve HPLC vials mune ino yezvinyorwa yakazara. Tsvaga nyanzvi insigo uye ruzivo rwakakosha rwekuwedzera yako chromato funko. Ongorora izvozvi! 50 Mibvunzo inowanzo bvunzwa paHPLC vials
Chirungu
ChiChinese
