Musoro Gasi Chromatography (HS-GC) inyanzvi yekuongorora ine jaine yekuongorora zvisingaite makomputa akasiyana siyana mumatanho emuenzaniso, kusanganisira zvinwiwa uye solids. Nzira yacho inobudirira inobvisa kuongororwa kuongororwa kwematanho akaomarara pasina kujekesa sampuli yese zvakananga mugesi chromatograp (GC). Panzvimbo iyoyo, HS-GC anoongorora chikamu chegesi pamusoro pemuenzaniso, inozivikanwa seMusoro. Iyi blog ichavhara zvese zvaunoda kuti uzive nezveMiscpace GC Kuenzanisira, kusanganisira nheyo dzaro, nzira, dzinobatsira, uye zviito zvakanakisa.

Unoda kuziva kuzere infor anoreva zvemusoro vial, ndapota tarisa ichi chinyorwa: Yakatemerwa Nhungamiro Kumusoro Musoro Viums: Zvimiro, Kusarudzwa, Mutengo, uye Kushandiswa

Kunzwisisa Musoro Sampling

Musoro Sampling anobva musimboti pane iyo musimboti uyo anovhenekera makomputa mumuenzaniso anogona kupatsanura muchikamu chegesi pamusoro pemuenzaniso painenge ichipiswa kana kuenzana. Maitiro acho anonyanya kubatsira kuongorora mishonga yechigadzirwa (VOCS), zvakasara sol soles, uye zvimwe zvinhu zvakaderera-zvinowira. Nzira dzemusoro dzinoderedza kusuma kwezvinhu zvisina-zvisingaenzaniswi uye zvinosvibisa muGC system, zvichikonzera kuchenesa Chromatograms uye zvimwe zvakavimbika.

Pfungwa dzinokosha dzeMusoro Sampling

Equilibrium:Kana sampuli yakaiswa mukati Vial yakavharwa Uye kupisa, mishonga inosvibisa ichatama kubva pamvura kana nzvimbo yakasimba kune chikamu chegesi kusvikira kuenzana yasvika. Kufunga kweye anawate mumusoro kunoenderana nekuderera kwayo uye tembiricha yemuenzaniso.

Kuparadzanisa Ceefficient (K):Iyo yekuparadzanisa coefficient chikamu chakakosha mune yemusoro sampling. Inotsanangura kugoverwa kwemakomputa iri pakati nepakati nepakati uye gasi zvikamu. Makomputa neakadzika k mavokari anowanzo kupatsanura nyore nyore mugesi yegesi uye saka zviri nyore kuonwa.

Static vs. Dynamic Musoro:Musoro Sampling inogona kuitwa uchishandisa chero nzira dzenyaya kana dzesimba. Musoro wenyaya unosanganisira kubvumira iyo semuenzaniso kusvika kuenzanisa mune yakasungwa vial, apo musoro wesimba unosanganisira kugara uchichenesa sampu ine gasi rakatsvaira kuti ratsvage muchikamu chegesi.

Musoro Gasi Chromatography Sampule Kugadzirira

Kugadzirira kwemuenzaniso kwakakodzera kunokosha kuti uwane ruzivo rwakakwana uye rwunoita mumusoro musikeprishing chromatography kuongorora. Matanho anotevera ataure nezve kufunga kwekutanga kwekugadzirira samasemu emusoro kuongororwa kwemusoro.

1. Sarudza iyo chaiyo samplu vial

Kusarudza kurudyisampuli vialyakakosha kune yakabudirira musoro sampling. Zviyero zveVaviri zvinosanganisira 6ml, 10ml, uye 20ml, ne20ml vialsKuve kwakanyanya kushandiswa. Zvinhu zvakakosha kufunga kana uchisarudza vials zvinosanganisira:

Chinyorwa:Vials vanowanzogadzirwa negirazi kana mapurasitiki.Girazi Valszviri nani samples inosimbisa nekuda kwekuita kwavo uye mukana wekusvibisa kusvibiswa kusvibiswa.

Kuongorora Mechanism:Vials inogona kuisirwa chisimbiso pamwe neyekuchema kana screw kapu. Crimp vialsIpa chisimbiso chemoltiorti, icho chinonyanya kukosha kuchengetedza kutendeka kwemusoro.

Septum mhando:ScAS yaishandiswa kusimbisa vials inogona kuunza zvinosvibisa kana ivo vari vane kunaka. Tarisa seSista yakagadzirirwa zvakananga kumumusoro mashandisirwo, sezvo ivo vasingakwanisi kusevha mumusoro.

2. Muenzaniso Vhoriyamu uye Dufiution

Iro vhoriyamu yemuenzaniso mune vial inokoshesa kuwana iyo yakakwana yemusoro wekusangana. Kazhinji, iyo sampula vhoriyamu inofanira kunge yakatenderedza 1 \ /3 kusvika 1 \ / 2 yehuwandu hwakazara hwesual kuti upe mupendero wakakwana wechikamu chegesi.

DILULItion:Kana kusvinura kwemuenzaniso kwakanyanyisa, kunogona kuguma nemusoro wakazadzwa, zvichitungamira kuhutsinye. Kutsiva sampuli ine yakakodzera solvent inogona kubatsira kuzadzisa iyo yaunoda kusunganidzwa kwekuongororwa kwechokwadi.

Unoda kuziva zvakawanda nezve nei mhirizi dzakashandiswa mu chromatography? Nei mishoms yemusoro inoshandiswa mu chromatography? 12 angles

3. Tembiricha kudzora

Tembiricha inoita basa rakaoma muMusoro Sampling sezvazvinoshamwaridzana nezvokuenderana neyekuongororwa uye kupatsanurwa kwavo muchikamu chegesi.

Kuenzana kupisa:Iwo ma samplu anofanirwa kuvezwa kune tembiricha inodzorwa kuti isimudzira kusunungurwa kwemishonga isina kujairika mumusoro. Iyo tembiricha yakakwana inotsamira pane chaiyo yekuongorora kuongororwa uye inofanira kutsanangurwa panguva yekuvandudza nzira.

Nguva yekuenzana:Bvumira nguva yakakwana yemuenzaniso kuti usvike kuenzana. Izvi zvinogona kusiyanisa zvichienderana nesemuenzaniso matrix uye kuderera kwemakomputa. Chaizvoizvo kuenzana kuenzana kubva pamaminitsi makumi matatu kusvika maawa akati wandei.

4. Regedza kusvibiswa

Kusvibiswa kunogona kukanganisa zvakanyanya kujekesa kwemusoro kwegirasi gasi chromatography. Kuti uderedze njodzi yekusvibiswa, ita zvinotevera:

Shandisa pre-yakacheneswa vials:Gara uchishandisa vakunda vasina kucheneswa kuti vadzivise kusvitswa kwekuzvisarudzira panguva yekurongedza kana kubata.

Nzira YEMAHARA:Mhanya nzira dzakabikwa kuti uzive zvinowanikwa zvekusvibiswa. Izvi zvinosanganisira kuongorora sampuro isina chinhu uchishandisa kugadzirira kumwechete uye maitiro ekuongorora kuti uone kuti hapana mapenzi asina kudiwa achizoonekwa muChromatorm.

Kudzora mamiriro ekunze:Ita kugadzirira kwekuenzanisa munzvimbo yakachena kuti ideredze kuratidzwa kune mhepo inosvibiswa.

5. Sarudza iyo chaiyo misoro nzira

Sezvambotaurwa, mumusoro sampling inogona kunge iri yemitambo kana ine simba. Iko kusarudzwa kwehunyanzvi kunoenderana nekunyorera chaiko uye chimiro chemuenzaniso.

Static Pompikoce:Iyi nzira yakakodzera kune akawanda mashandisirwo uye anoshandiswa zvakanyanya kuongorora mishonga inoshamisa mune zvinwiwa uye solids. Izvo zvinobvumira ongororo kune zvakasikwa kupatsanurana mumusoro pasina iyo yekutanga kwegadziriro yekuwedzera.

Dynamic Musoro:Iyi nzira inokodzera zviri nani samples dzinoda kuenderera mberi kuchenesa kuendesa mishonga inosimbisa. Izvo zvinowanzoshandiswa mukushandisa zvakadai sekuongororwa kwezvakatipoteredzwa uye kuongororwa kwechikafu uko samples inogona kuve neyakaderera fungidziro yekuongorora.

Zvakanakira zvemusoro weGC sampling

Kuchenesa Chromatograms:Nekuongorora chete chikamu cheMapor chete, misoro sampling inoderedza kuiswa kwezvinhu zvisiri-zvisingaenzaniswi uye zvinosvibisa, zvichitungamira kuchenesa Chromatograms uye nekuvandudza mafungiro ekuongorora.

Yakadzora Sampula Nguva Yekugadzirira Nguva:Musoro Sampling Reser Rinorerutsa maitiro ekugadzirira, sezvo ichibvisa kudiwa kweiyo yakaoma yekubvisa kana maitiro erondedzero.

Kuwedzera kunzwa:Kusvina kwekunyatsoongorora mumusoro kunogona kutungamira mukuvandudzwa kwekunzwa, zvichiita kuti zvive nyore kuona trace-level compound.

Kuedza:Musoro sampling inogona kuiswa kune dzakasiyana siyana dzemhando dzakasiyana siyana dzemhando, kusanganisira zvinwiwa, zvinokonzeresa matrices, zvichiita kuti ive nzira dzakasiyana siyana dzekushandisa dzakasiyana siyana.

Unoda kuziva zvakawanda nezve ratidziro yekumanikidza yeAjiirn HeadPe vials uye Caps, ndokumbirawo utarise izvi zvinyorwa: Ndeipi mwero wekumanikidza kweAijiren Musoro Varume uye CAPS?

Mhedziso

Musoro Gasi ChromatographyIcho chinhu chakakosha ingafungo chekuongorora kwekuongororwa kwehuremu hwemhando dzakasiyana mumatomu akasiyana siyana mumatanho akasiyana. Kugadzirira kwakakodzera kwekuenzanisa kwakakosha kwekuzadzisa zvakaringana uye mukubereka mibairo muzvinyorwa zveGC ongorora. Nokunzwisisa nheyo dzemusoro weMusoro Sampling uye kutevera maitiro akanakisa ekugadzirira kwemuenzaniso, mabharabhu anogona kusimudzira kushanda uye kuvimbika kwekuongorora kwavo. Sezvo kudikanwa kwehunyanzvi hweMhando yepamusoro ongororo dzinoenderera mberi kuti ukure, Musoro GC sampling icharamba iri chishandiso chakakosha kune vanoongorora uye vanoongorora akawanda marangi.

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