High-performancegraphy chromatography (HPLC) uye Liquid chromatography-misa misa (lcms) ndedzimwe nzira dzine simba dzinoshandiswa muminda dzakasiyana-siyana, kusanganisira nesainzi, uye neyenzvimbo yezvakatipoteredza, uye nehesi rezvemamiriro ekunze. Nepo nzira mbiri idzi dzinosanganisira kupatsanurwa uye kuongororwa kwemakomputa, nheyo dzadzo, mabasa, uye zvinoshandiswa zvakasiyana. Iyi blog post ichaenzanisa HPLC uye LCMS, kutarisa pane yavo yakakosha misiyano uye zvakanakira maitiro ega ega.

Nheyo yekuvhiya

Hplc

HPLC ndeye chromatographic maitiro ayo anopatsanura makomputa anoenderana nekudyidzana kwavo neiyo stationary uye nhare mbozhanhare. MuHPLC, iyo Liquid Mobile chikamu inovhuniwa kuburikidza nekamuri yakazadzwa nechikamu chenzvimbo, chinowanzoumbwa nezvikamu zvidiki zveSilica kana polymers. Kana musanganiswa wemuenzaniso waunosvitswa muhurongwa, izvo zvikamu zvakaparadzaniswa zvichienderana nehukama hwavo hwenzvimbo yekutandarira. Compounds inowirirana zvakanyanya neyekuita chikamu cheElute zvishoma zvishoma, nepo makomisheni anodyidzana asinganzwisisike kushamisika zvakanyanya. Iwo akapatsanurwa makomputa anobva aonekwa achishandisa akasiyana-siyana ekuona, akadai seUV-vis, fluorescence, kana kudzora index yekuona.

Unoda kuziva zvakawanda nezvezvinoshandiswa zve chromatography vials, ndapota tarisa chinyorwa ichi:15 zvikumbiro zve chromatography vials muminda dzakasiyana

LCMS

LCMS inosanganisa kupatsanurana kwekuparadzanisa kweHPLC ine huwandu hwekuongorora huwandu hwekugona kwepfungwa. MuLCMS, samples inotanga kupatsanurwa uchishandisa HPLC, kungoita seyakajairika HPLC. Nekudaro, panzvimbo pekuonekwa newakawonekwa neanoona akajairwa, mishonga yehukama inounzwa mune misa masaltrometer. Iyo Mass SpectRometer ionizesa makomputa uye kuyera yavo yakawanda-kune inobhadharisa ratio, inopa ruzivo rwakadzama pamusoro pehuremu hwemanyorerwe uye chimiro chemufundisi. Uku kusanganiswa kwekuparadzanisa uye misa misa spectrometry kunopa kunzwa kukuru uye chokwadi pane HPLC yega.

Kuparadzanisa Mechanism

HPLC:HPLC inoshandisa yakanyanya kumanikidza kusundira iyo Liquid Mobile chikamu kuburikidza nekhombwe yakarongedzwa ine chikamu chakamira. Makomputa akapatsanurwa zvichibva pane yavo yekusiyanisa yekudyidzana neiyo nzvimbo yekunongedza, zvichikonzera kupatsanurana uye kunyatsoparadzanisa.

LCMS:LCMS inosanganisa kupatsanurana kwepanyama kweHPLC ine misa masendimita ekugona kwepfungwa emisangano misa (ms). Iyo yemvura chromatography component inoparadzanisa makomputa, nepo misa masaltrometer inoyera huwandu-kune-kubhadharisa chiyero cheiyo ionized mhando.

Kunzwa uye kusarudza

HPLC:HPLC ine pfungwa dzakanaka uye kusarudza, kunyanya kana ikabatanidzwa nevakuru vanoona senge UV-vis, fluorescence, kana diederescence. Nekudaro, HPLC yega inogona kusapa chokwadi chakakwana kune yakaoma samples.

LCMS:LCMS ine SUPS Sensitivity uye Kusarudzwa Kuenzaniswa neHPLC. Masiyano mashure anogona kunyatsozivisa uye kuwanda mishonga yakavakirwa pane yavo yakasarudzika misa-to-kune inobhadharisa ratios, kunyangwe mune yakaoma matrices. Izvi zvinoita kuti LCMS inonyanya kubatsira kuongorora makomisheni uye kusimbisa kuzivikanwa kwezvinhu zvisingazivikanwe.

Kuenzanisira Kugadzirira

HPLC:Sampula Kugadzirira kweHPLC kazhinji inosanganisira dilution, filtration, kana nzira dziri nyore dzekuratidzira. Sampuli haidi kuiswa, saka HPLC yakakodzera kune yakasiyana siyana makomputa, kusanganisira polar uye zvinhu zvisina kunaka.

LCMS:Sampule kugadzirira kweLCM yakafanana neiyo yeHplc, asi matanho ekuwedzera anogona kudikanwa kuti ave nechokwadi chekuenderana neye misa spectrometer. Semuenzaniso, vashoma buffers kana vanowedzera vanogona kudikanwa kuti vasimudzire ionsization uye kudzivirira ion kudzvinyirira.

Zvishandiso

HPLC:HPLC inoshandiswa zvakanyanya kuongororwa kwemishonga yemishonga, chikafu, uye masampuzhinji emunharaunda. Izvo zvinonyanya kushanda kuti uparadzanise uye kufungidzira makomputa mumasanganiswa akaoma, sekusvibiswa kwezvinodhaka, zvinopesana nezvikafu, uye zvinopedza porticides.

LCMS:LCMS ine huwandu hwakawanda hwekushandisa, kusanganisira:

Kuongorora kwechechi: Inoshandiswa mukuwanikwa kwezvinodhaka, kukura, uye kukanganisa kwakanaka.

Previomics uye metabolomics: yaishandiswa kuziva uye kuwanda mapuroteni uye metabolites mune yezvinhu zviyero.

Kuongorora kwezvakatipoteredza: Waimboona tsanga dzakasvibirira mumvura, ivhu, uye ma samples.

Kuda kuziva nezve basa reCertified HPLC vials mukuona kwakaringana kuongorora? Verenga chinyorwa ichi: Iko kukosha kweCertified HPLC vials kune chaiyo samples kuongorora

Mhedziso

HPLC uye LCMS ndeye ese ane simba ekuongorora maitiro nemafungiro avo uye zvisingakwanisi. Sarudzo pakati pezviviri zvinoenderana nezvinodiwa zvekuongorora, sekunzwa, kusarudza, kuenzanisa kuoma, uye zviwanikwa zviripo.

HPLC isarudzo yakanaka yekuongorora maitiro ezvinyorwa zvinozivikanwa mumatanho akareruka, uko mutengo uye kurererwa kwekushandisa zvakakosha zvinhu. LCMS, kune rumwe rutivi, ndiyo nzira inosarudzwa yekuongorora maitiro akaomarara, inozivisa zvisingazivikanwe mishonga, uye kuzadzisa Ultra-trace level yekuona.

Sezvo tekinoroji inoramba ichisvika, kuunganidzwa kweHplm uye lcm kuchatamba basa rinowedzera mukusangana neyekuvandudza madhiri ekuongorora kwemakemikari ekuongorora uye anoverengera migumisiro yakanyanya kuwanda.

Yapfuura:

Iyo yekupedzisira gwara kune 0.45 micron syringe mafirita iwe

Zvese zvaunoda kuti uzive nezveMusoro weGC Sampula Kugadzirira