Murabhoritari yemazuva ano yekuongorora, chromatography inopfuura nekure pane routine protocol; kutamba kwakaoma kunzwisisa kwekudyidzana kwema molecular. Kunyangwe iwe uri kuyera kusvibiswa kwemishonga kana kuona zvinosvibisa kwezvakatipoteredza pamatanho ekuteedzera, kunzwisisa iyo yakakosha fizikisi yekuparadzanisa kwako ndiko kunosiyanisa nyanzvi kubva kuna master chromatograph.
Kubudirira mulabhu hakungouyi kubva kune yakakwirira-yekupedzisira zvigadziriso zvezviridzwa. Iyo inogara mukubatana pakati penzira yako chemistry uye yepamusoro-yemhando yekushandisa inodzivirira yako sampuli kutendeseka. Kune avo vanofambisa zvinonetsa zve
HPLC vs LC-MS: Ndeipi Yekusarudza
, kana kurwisana nenheyo dzisina kugadzikana, gwara iri rinogovera zvakavanzika-padivi zvakavanzika kuti uwane data-yepasi data uye kuwedzera chiridzwa uptime.
Kunzwisisa Hydrophobic Handshake: Chii chinonzi Reverse Phase Chromatography?
Kupindura mubvunzo wekutanga-chii chinonzi reverse phase chromatography-munhu anofanira kutarisa kupfuura tsananguro yebhuku. Pakati payo, ndiko kuparadzaniswa kunobva pa "kutya kwemvura" (hydrophobicity). In reverse phase high pressure liquid chromatography (RP-HPLC), isu tinoshandisa isiri-polar stationary phase uye polar mobile phase kuronga mamorekuru neawo hydrophobic hunhu.
Iyo Chemistry yeC18 (Octadecyl) Chain
Iyo yakajairika yakamira chikamu inosanganisira silica-based particles akasungwa neC18 (octadecyl) cheni. Kubva pane zvakaitika kwandiri, kuwanda kweaya macheni eC18 uye mhando yerutsigiro rwesilica inotaridza kuoma kwenzira yako. Vazhinji vaongorori vanosangana nepeak tailing kana vachiongorora zvakakosha makomisheni. Izvi zvinowanzokonzerwa nemapoka e-silanol akasara pane silica pamusoro anoita seasingadiwi ion-exchange sites.
Kuti tigadzirise izvi, isu tinoshandisa iyo yekupedzisira-capped column, apo madiki silanes "mask" aya anoshanda masaiti. Nekudaro, kunyangwe iyo mbiru yakanaka haigone kugadzirisa jekiseni rakashata. Kana yako chromatographic peak ichiita seyakamonyaniswa isati yatomborova koramu, tarisa vial yako. Kushandisa high-purity
9mm Short Thread Vials ine Universal Fit
inova nechokwadi chekuti analyte yako haishambadzire kumadziro evhavha isati yatomboitora.
Iyo Art yeOrganic Modifier muHPLC Method Development
Iyo nguva yekuchengeta yevaongorori vako inotongwa neiyo organic modifier HPLC yevasungwa. Nekugadzirisa chiyero cheAcetonitrile kana Methanol kune yako aqueous buffer, iwe unoisa iyo "elution simba" yechikamu chenhare.
Gomba rakajairika randinoona mukufambisa nzira kusanzwisiswa gradient elution curve. Kana iwe ukakwidza yako organic modifier nekukurumidza, unorasikirwa nechisarudzo; zvishoma nezvishoma, uye unotambura nekuwedzera kwepamusoro uye kupera simba rekunyungudutsa. Kana uchichinja pakati
Analytical vs Preparative HPLC
, kutonga kutsetseka kwe gradient kunova chinhu chikuru mukubudirira kukwira-kumusoro. Rangarira, Acetonitrile inopa yakaderera viscosity uye yakakwirira elution simba, nepo Methanol inogona kupa akasiyana sarudzo ye polar makomisheni iyo ACN inogona kupotsa.
Kududzira GC Elution Order: Kufanotaura Compound Separation
Kufanotaura gc elution kurongeka nderimwe rematambudziko ane mubairo kune gasi chromatographer. Kusiyana neHPLC, uko solvent chemistry ndiyo yekutanga lever, GC kupatsanurwa kunorairwa ne "Tiriniti Yekuparadzanisa": nzvimbo yekubikira, molecular polarity, uye column tembiricha hurongwa.
Thermodynamics vs. Dipole Interactions
Muchikamu chisiri-polar chakamira, iyo gc elution kurongeka inoteedzera painofashaira. Makomisheni akanyanya kushanduka anobuda kutanga. Nekudaro, mutambo unochinja paunoshandisa polar column sePEG\/Wax. Ini nguva pfupi yadarika ndakabata purojekiti inosanganisira isomers uko mapoinzi ekubikira akada kufanana. Nekuchinjira kune polar phase, iyo dipole-dipole kudyidzana kwakatibvumira kuparadzanisa aya makomisheni zvichibva pane yavo yemagetsi chimiro pane saizi yavo.
Kune basa repamusoro-sevhisi senge ongororo yekuongorora kwezvakatipoteredza, kutendeseka kwechigadziko chako chemuenzaniso kwakakosha. Kushandisa
20mm Crimp Pamusoro Musoro Musoro
inodzivirira kurasikirwa kweanoshanduka anoongorora, kuve nechokwadi chekuti yako yakawanda spectral deconvolution yakavakirwa pachiratidzo chechokwadi chemuenzaniso wako, kwete chikamu chakaburitswa.
HPLC vs LC-MS: Kufambisa iyo Sensitivity Shift
Mubvunzo unowanzo murabhoritari yedu ndewekuti: "Tinofanira kubva riini kubva kuHPLC vs LC-MS?" Mhinduro iri mukunzwa kwako kunodiwa. Nepo HPLC ine UV yekuona yakanakira kujaira QC pamazinga emicrogram, LC-MS inodiwa kana iwe uchida kuona mapicogram mazinga kana kuziva zvisingazivikanwe mumatrices akaomarara.
Kana uri kutamira kuLC-MS, sarudzo yako yemabhodhoro inotonyanya kukosha. Makapu akajairwa anogona kupinza mapurasitiki muchikamu chako chenhare, achigadzira "Ghost Peaks" inoshungurudza chromatogram yako. Ichi ndicho chikonzero Aijiren's
Bonded Screw Caps
Inonyanya kukosha muMS-certified labs-inobvisa njodzi yekuti septa inowira muvharosi kana leaching zvinosvibisa mudetector.
Iyo Anatomy yeYakakwana Chromatographic Peak
Yese chromatographic peak chishandiso chekuongorora. Yakapinza, inofananidzwa Gaussian peak inokuudza kuti system yako yakagadziridzwa. A "fudzi" kana "muswe" inokuudza kuti chimwe chinhu chakashata, kazhinji chine chekuita nehardware kana vhoriyamu yakafa.
Kubvisa Yakafa Vhoriyamu uye Yekuwedzera-Column Vhoriyamu
Imwe yeakanyarara mhondi yekugadzirisa ivhu rakafa. Kana paine chero gaka pakati petsono yako uye pasi pevhavha, sampu inopararira. Kune micro-sampling, ini ndinogara ndichikurudzira kushandisa Conical Inserts. Izvi zvinopinza zvinomanikidza sampuli kuti ive yakamanikana, yakatwasuka nzira, ichiva nechokwadi chejekiseni "rakapinza plug". Izvi zvinokonzeresa zvakanangana nepamusoro-soro uye zvirinani chiratidzo-ku-ruzha reshiyo, kunyanya mumasisitimu ekudzvanya kukuru uko kupararira ari muvengi.
Kugadzirisa matambudziko sePro: Kubva paBaseline Noise kuenda kuSepta Coring
Mumakore angu ekugadzirisa matambudziko, ndakaona kuti 70% yenyaya dzezviridzwa inyaya dzinodyiwa.
-
Baseline Noise & Ghost Peaks: Kana iwe ukaona nhongonya painofanira kunge pasina, mafirita ako anogona kunge ari honzeri. Nguva dzose tarisa kune
Iyo Yakazara Yekutungamira kune 0.22 Micron Mafirita
usati wasarudza sefa yako sirinji.
-
Septa Coring: Kana iwe ukaona mabheti esilicone mubhodhoro rako, tsono yako iri "coring" iyo septa. Izvi zvinoitika nemakapisi asina kunaka. Yedu ND11 Crimp Caps uye 10-425 Screw Caps yakakwenenzverwa-engineered kuti imirire akawanda punctures pasina kupatsanuka.
-
Kugadzikana muKuchengetedza: Kune nzira dzeEPA kana kuchengetedza kwenguva refu, uchishandisa
24-400 EPA Vials
yakagadzirwa negirazi yeborosilicate inovimbisa zero solvent kurasikirwa uye zero kusvibiswa.
Nei Data Kutendeseka Kunotanga neConsumable
Isu tinowanzo shandisa 50,000 USD pane yakakwirira-yekupedzisira system, tozoedza kuchengetedza pennies pabhodhoro. Mune ruzivo rwangu, sub-par vial ndicho chinhu chinodhura zvakanyanya mulabhu yako nekuti inotungamira kune "kumhanyisazve" uye "kutadza kusimbiswa."
Kunyangwe iwe uchida 1ml Shell Vials ye nyore chromatography kana 18mm Screw Thread Headspace Vials yeotomatiki GC, Aijiren inopa kuenderana kunodiwa nenyanzvi. Isu tinoita shuwa kuti yako gc elution odha inodhinda zuva nezuva, uye yako chromatographic peak inogara yakapinza sezuva rawakagadzira nzira.
Tsigiro yeNyanzvi & Kubvunza: Uri kutarisana nemhedzisiro isingaenderane kana kunetsekana nekuvandudza nzira? Regedza kufambisa kwako kushanda pamwe chete. Ndibate kuti uwane kubvunzana nezvehunyanzvi:
Mhedziso Kubata icho chiri reverse chikamu uye kugona nuances yechiridzwa chako rwendo rwekuenderera mberi nekudzidza. Nekusarudza iyo chaiyo organic modifier HPLC uye zvakanakisa-mu-kirasi zvinoshandiswa, uri kuisa mari muchokwadi chedata rako. Usarega sub-par vial ichiparadza nzira yakanaka yawakapedza mavhiki uchigadzira.
English
Chinese
