Filter breakthruc inyaya yakaoma mune syringe filtation iyo inogona kutungamira kune sampula yakasvibiswa uye kukuvadza kune zviridzwa zvekuongorora zvakadai seHPLC. Kuti udzivise kubhururuka kwekubhururuka, zvakakosha kusarudza kodzerosyringe firitauye tevera maitiro akanakisa panguva yekuita kwefaira. Heano mamwe matipi akakosha:

Mibvunzo inowanzo bvunzwa nezve syringe mafirita, ita shuwa yekuongorora ichi chinyorwa chinodzidzisa: Iyo musoro we "syringe filter" 50 mibvunzo inowanzo bvunzwa

Sarudza yakakodzera faira yefaira uye saizi yepora

Sarudzo yefaira yefaira uye saizi yepora zvinoenderana nemhando yemuenzaniso wako:

Zvemhinduro dzemvura uye yakawanda organic solventes, hydrophilic inozorwazve cellulose (RC) mafirita isarudzo yakanaka.

Dzivisa mafirita ane pfas (per- uye polyfluoroalalalkyl zvinhu) izvo zvinogona kusekerera mumuenzaniso.

Funga nezvezvinwiwa zvinoshanda mumuenzaniso wako uye sarudza zvinhu zvefaira zvinoenderana uye zvakasununguka zvekubvisa.

Zvekunyorera zvakawanda, 045 μM pore saizi yakakwana kubvisa zvimedu. Zvidiki zvidiki zvidiki saizi senge 0,2

Shandisa prefilter yemasampu ane zvinonetsa

Prefilters akaisa kumusoro kweiyo firita yemhepo inogona kubatsira kudzivirira kubudirira nekudzora mutoro wezvekuita microbic uye kubvisa zvimisikidzwa zvinogona kukonzera kudzvinyirira kuchinjika:

Adsorptive yakadzika mafirita anoshanda prefilters ekuratidzira mafirita ekurwara, kubvisa zvidiki zvekuunganidza anogona kuburitsa mvura yekunyora.

Uchishandisa 0.5 μm prefilter yakadzivirira kubudirira kweS. Marcescens kuburikidza0.2 μm mafiritaMune kumwe kuedza.

Nekudaro, prefilters inowedzera mutengo uye kuoma, saka kushandisa kwavo kunofanira kururamiswa zvichibva pane chaiyo application.

Iwe unozoshandisa izvi syringe mafirita, iwe unoziva here kuti iyo syringe yefaira inogona kushandiswazve? Ndokumbira utarise chinyorwa ichi: Zvekusvibisa mafirita, uchasimbisa?

Simbisa maitiro efaira

Maitiro akakodzera ndiwo kiyi yekudzivirira kubudirira:

Filter samples pakarepo usati waongorora kuti uderedze kukura kwemicrobial.

Shandisa nzira yakapfava yakaita kunge kumanikidza kwete yekuvhara kuti udzivise kuzvimba masero.

Filter mavheti akakwana kuti uone mwero wakaderera wekusvibiswa - kanokwana zana ml mune imwe chidzidzo. Iyo USP inokurudzira kuyedza 10% ye batch vhoriyamu.

Dzivisa kuunganidza samapu dzakaerekana dzakatambanudzwa nguva dzaigona kubvumira kukura kwemicrobial.

Simbisa maitiro eFilt

Kuedza kusimbiswa kwakakosha kuti uve nechokwadi chekuti maitiro ekushaya basa anoshanda kune imwe neimwe application:

DAMBUDZIRA MABHUKU NEZVINHU ZVINOGONESESA DZESVONDO DZESVONDO UYE ZVINOGONESESWA HERE, kwete chete zviyero zvebvunzo.

Tsika dzine nzara dzinogona kunge dziri mumiriri hwakawanda hwemamiriro ezvinhu epasi rese pane hutano hwakanaka-hwakapfuma.

Ongorora iyo yese filtration maitiro, kwete chete puthete pachako.

Ita shuwa zvakakwana microbial microbial microbial mazinga uye sampula mavhoramu kuti uone breakthruct.

Shandisa yakakwirira-mhando syringe mafirita

KusarudzaSyringe mafiritaKubva mukunyaradza mugadziri kunogona kupa imwe vimbiso yekuti firita yekubudirira haizoitika:

Tarisa mafirita ane maficha akadai sekupisa-kusimbisa membranes ayo anodzivirira kusunungurwa.

Musoro wakakwana mukati meimba yefaira kuti ugare makiromita akakura emaketi usati wabvisa.

Kukodzera kwekuita kwefaira (i.e., HPLC) inozivisa mhando.

Nekutevera aya maitiro akanakisa ekusarudza kwefaira, process optimization, uye yekuedza kusimbiswa, iwe unogona kuderedza njodzi yekubudirira kwefaira uye uve nechokwadi chekuvimbika kwemasampula ako uye mhedzisiro yekuongorora. Kudyara mumhando yepamusoro-mhando syringe firita kubva kune yakavimbika mugadziri zvakare zvakare chengetedzo yakakosha.

Unoda kuziva ruzivo rwakakwana nezve syringe firita, ndapota tarisa ichi chinyorwa: Yakatemerwa Nhungamiro kune Syringe Mafirita: Zvimiro, Kusarudzwa, Mutengo, uye Kushandiswa

Yapfuura:

Ndeipi buffer yakanakira yako HPLC mobile chikamu?

Ndeipi basa reCOD bvunzo tubes mukuongororwa kwezvakatipoteredza?