Kusarudza iyo chaiyo buffer yeHPLC nhare mbozha ndeyekutsoropodza kuwedzera kupatsanurwa uye kuongororwa kwako samples. Buffers inobatsira kuchengetedza iyo yakagadzikana pH, iyo yakakosha kudzora iyo ionization mamiriro ekuongorora kwako, nokudaro achikanganisa nguva yavo yekuchengeta kwavo uye peak. Pazasi pane yakadzama tarisiro yekufunga kwekusarudza yakanakisa buffer yeHPLC yako application.

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Kukosha kweBuffer Kusarudzwa

A Buffer ndiyo mhinduro ine iyo pH haina kuchinja mushure mekuwedzera zvishoma huwandu hwe acid kana chigadziko. Zvichienderana nekuumbwa kwavo, buffers inogona kuiswa seyakangoita acidic kana yekutanga. Kubudirira kweBuffer kunotemerwa nePTA iyo, iyo inofanirwa kuve mukati meiyo

Zvinhu zvakakosha kufunga nezvazvo

1.Ph Range: Iyo pH yeDeal phare inofanira kunge iri angangoita 2 Ph kuyamwisa kubva paPnda yekuziva. Izvi zvinobatsira kudzvinyirira hunhu hweAidic of acidic kana basict analyte, kuwedzera kwavo kuchengetwa kwekoramu. Semuenzaniso, kana ana ana fungidziro isina kusimba acid ne p tik of 4,5, ichishandisa nhare yekirinari ine pfi kutenderedza 2.0 yaizochengeta mune isiri-ionized State, kuwedzeredza kuchengetwa.

2.Buffer mhando: yakajairika buffers inoshandiswa muHPPLC inosanganisira:

Phosphathe Buffers: Inoshanda muPP Range ye2,0 kusvika 8.0, phosphathe buffers inoshandiswa zvakanyanya nekuda kwekugadzikana kwavo uye kuenderana nevakawanda vanoongorora zvakanyanya. Ivo vanonyanya kukodzera kuLC-UV zvikumbiro.

Acetate Buffers: Izvi zvakare zvinozivikanwa zveLC-UV assays uye inogona kushandiswa mune yakafanana Ph

Volile Buffers: yeLC-ms application, volatle buffers sekuumbwa, acetate, uye ammonium bicarbonate inodiwa nekuti ivo vanogona kubviswa zviri nyore panguva yekuita masendimita.

3.Buffer Kusvina: Yakajairwa Yekutanga Yekutangisa yeBuffers muHplc iri pakati pe25-50 mm. Nzvimbo dzakakwirira dzakakwirira dzinogona kuvandudza peak chimiro uye kushanda zvakanaka asi zvinogona zvakare kutungamira kuKwunyuka kana kusanganiswa ne organic sol sol. Zvinokurudzirwa kuchengeta iyo buffer yekuvhara pazasi makumi maviri nemashanu mm kuti udzivise matambudziko.

4.chemical kuchena: gara uchishandisa yakakwirira-yekuchena chromatographic-giredhi buffers kudzivirira kusvibiswa uye kuve nechokwadi chakavimbika mhedzisiro. Kusvibiswa kunogona kutungamira kune zvisingatarisirwi zvekudyidzana uye kusiyanisa mune dzakatendeseka nguva.

Tembiricha mhedzisiro: Tembiricha inogona kupesvedzera iyo pH yeBuffer Solutions. Izvo zvakakosha kugadzirira uye kuchengeta mabhegi pakudziya kusingashandi kuchengetedza kushanda kwavo.

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Mazano anoshanda eBuffer Kugadzirira

Kugadzirira nzira: Buffers inogona kuve yakagadzirirwa nekusanganisa yakaenzana huwandu hwezvakaratidziro zvadzo uye zvekutanga kana nekufunga kwekugadzirisa kweiyo acid. Dzivisa kugadziridza iyo ph mushure mekuwedzera organic sols, seizvo izvi zvinogona kutungamira kumhedzisiro isingaenderane.

Mhinduro nyowani: Gadzirira mitsva yekudhura nguva dzose kuti uderedze kukura kwemicrobial uye kusvibiswa kwezvikamu, izvo zvinogona kukanganisa chromatographic.

Kudzivisa Corrostion: Dzimwe Buffers, dzakadai seCitrate, inogona kuora stainless simbi zvikamu zveHPLC Systems. Ita shuwa kurova kwehurongwa mushure mekushandisa zvakadai buffers kudzivirira kukuvara.

Mhedziso

Kusarudza iyo chaiyo buffer yeiyo HPLC yako nhare chikamu ndeyekutsoropodza kuwana yakavimbika uye mubereki mibairo. Funga nezvePH, mhando yeBuffer, kusangana, uye kuchena kana uchisarudza. Nekutevera nhungamiro idzi, iwe unogona kuongorora yako HPLC nzira yekunyatsoparadzanisa uye kuongorora masampula ako.

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