I-HPLC yindlela eluncedo yokuhlaziya. Isetyenziswa kwimimandla emininzi yenzululwazi. Phakathi kwezi ziindawo zeyeza, uhlalutyo lokusingqongileyo, kunye nokhuseleko lokutya. Babalulekile kwinkqubo yohlalutyo. Ke ngoko, ukukhetha i-vial vial ibalulekile. Ichaphazela ukuchaneka kunye nokuthembeka kweziphumo.

I-HPLC VialsGcina iisampulu zakho zikhuselekile kungcoliseko kunye neempembelelo zangaphandle. Ungafumana ezi vils kwiindidi ezininzi, ubungakanani, kunye nezixhobo. Ziyalungela iindidi ezahlukeneyo zesampulu kunye neemfuno zohlalutyo. Uhlobo ngalunye lwe-HPLC lubonelela izibonelelo ezizodwa. Zineempawu ezinceda uhlalutyo lwakho luphumelela.

I-vials ye-HPLC ineenjongo ezimbini eziphambili. Bagcina iisampuli ezimsulwa ngokunqanda ungcoliseko kwaye bazinzile. Ungcoliseko lunokuba nezizathu ezahlukeneyo. Umzekelo, ibhotile yebhotile inokukhupha. Umphezulu webhotile unokunxibelelana. Okanye, i-vial inokuba nengcungcutheko. Ukulungisa le micimbi, abavelisi basebenzisa iglasi ekumgangatho ophezulu. Inomxholo weBoron oPhezulu kwaye yeyokwenza isampulu ye-HLLC. Bahlangabezana nenqanaba eliphambili lobugcisa. Le migangatho igubungele ububanzi bayo kwaye bangaphandle komlomo we-valm kunye nomzimba. Bakugubungela ukuchaneka komlomo onxungupheleyo. Bahlangabezana nazo zonke iimfuno zeMigangatho yeHlabathi. Imveliso nganye yemveliso iphantsi kolawulo oluphambili. Sinengxelo yovavanyo lwefektri. Sinciphise umngcipheko wongcoliseko lwesampulu.

Ndinomdla malunga ne-vials ye-HPLC? Fumana iimpendulo ezingama-50 ezineenkcukacha kweli nqaku:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Enye into ephambili kukuba iyahambelana nezinyibilikisi kunye nezigaba zesoftware kuhlalutyo. Izixhobo ezahlukeneyo ze-Vial zinokungaxhathisi beekhemikhali ezahlukeneyo kwizinyithi ezinobuchule okanye amaqondo obushushu aphezulu. Ukukhetha izixhobo ze-Vial ezifanelekileyo Gcina i-vial yokuqhekeka. Ikwathintela ukonakala kwesampulu okanye ungcoliseko.

Inkqubo yokuvalwa kwe-Vial ikwabalulekile ukugcina isampula ikhuselekile. I-Septa idla ngokwenziwa kwi-ptfe kunye ne-silicone, ethintela ukuvela nokunciphisa umngcipheko wongcoliseko. Ukutywinwa kakuhle kuyaqhubeka isampulu. Esi siphambili ngexesha lohlalutyo. Ingakumbi ixesha elide okanye iziko eliguqukayo.

Isampulu ye-Vials

Uninzi lwesampulu yenziwa ngeglasi. I-USP (i-United States PharmacopeIa) ihlela iglasi yelebhu ngokuchasene namanzi.

1. Chwetheza i-I, 33

I-Borossililicicate ye-Borossililicate ye-gubi ye-gesi yohlobo loku-1, iBakala A, eyi-33 liyinto engaphezulu kwaye isetyenziswe ngokubanzi kwi-labhoratries, ngakumbi kwiChromatography, kwaye iqulathe i-silicon neoksijini. Inesisombululo esisezantsi kunye nokwandiswa komgangatho wokulandelelana kwe-33.

2. Chwetheza i, iglasi eyandisiweyo eyi-51 eyandisiweyo

Le glasi ibizwa ngokuba yi-borossilicate ye-gesi yohlobo lwe-USP 1, iklasi B, 51. Yenziwe kakhulu ngesilika kunye neoksijini. Inexabiso elincinci le-boron, isodium, kunye neentsimbi ezininzi i-alkali kune-Glass. Kodwa, isenokusetyenziselwa iilebhu. Zonke ii-vials ze-Amber zii-victs zeklasi ze-vials ezinomsebenzi olungiselelwe ukwanda komgangatho wama-51.

I-3. Polypropylene (PP)

I-PP yiplastiki engasebenziyo. Inomchasi ofanelekileyo weekhemikhali. Ilungele ukugcinwa kwexesha elifutshane leekhemikhali zelebhu. Ingasetyenziswa apho iglasi ayisiyo inketho. Xa usebenzisa i-hydrocarbons evuthayo okanye ebingaqhelekanga, ukumelana nokuncipha ngokuhamba kwexesha.I-PP Vialszisetyenziswa ngokubanzi kwi-ion chromatography. Kungenxa yokuba bane-ion ephantsi kwaye banokucocwa kunye namanzi abuthathaka acid kunye namanzi e-deoer. I-polypropylene vials igcina i-caps yabo emlilweni. Oku kunciphisa ukuvezwa kwezinto eziyingozi. Elona qondo lobushushu liphezulu yi-135 ° C.

Ukukhetha iimodeli zesampulu zenziwe ngezinto ezahlukeneyo

a. Iglasi-isebenza ngokupheleleyo kwaye ichasa i-acid;

b. I-amber-kwiisampulu ezibuthathaka zokukhanya;

c. I-Polypropylene - iisampulu ezinxilisayo okanye izicolo ezinyibilikayo amanzi;

d. Ukufakwa kwemifanekiso emincinci-iyinto encinci kakhulu inaliti;

e. Ukuvuselela okuphezulu-iziisampulu zesampulu;

Ndinomdla malunga nezibonelelo zeglasi yeglasi yeglasi ngaphezulu kwezinye iindlela zeplastiki? Jonga inqaku lethu lokufumanisa izinto eziphambili ezenza iglasi ukhetho oluphezulu kwizicelo zakho zeChromatography: Izizathu ezi-3 eziphezulu zokuba kutheni iGlasi chromatography ye-vials ilunge ngakumbi kune-vials yeplastiki

Iindidi zeemodeli zesampulu

Ii-vials zesampulu ziqhele ezahlulwe zaba ziindidi ezintathu. Ezi zezi: crump cap, isikhafu i-cap yentamo, kunye ne-snap i-vials vials.

Ungayikhetha njani phakathi kwezi vils ezintathu zesampulu?

Zintathu iindidi zeCAPS ezifumanekayo kwii-vials zesampulu: i-crmimp caps, i-snap caps, kunye neCAPS ye-screw. Indlela nganye yokutywina ineengenelo zayo.

I-1. Crimpp ephezulu yentloko

I-CRIMP ephezulu yentloko inyikima i-septum phakathi komphetho wesampulu yeglasi kunye ne-aluminium ye-aluminium. Iziphumo zokutywina zilungile kwaye zithintela ngokufanelekileyo isampulu. I-Septum ihlala ikho. Inaliti ihlambe isampulu. UkutywinwaI-crimp cap vialsifuna ukusetyenziswa kwe-capper. Inani elincinci leesampulu, iCapper yeNcwadi lelona khetho lilungileyo. Kwisixa esikhulu sesampulu, i-capper esenzekelayo inokusetyenziswa.

I-2. Screw i-cap yentamo

II-screw inkcitho yentamoyindawo yonke. Ukuqinisa i-cap kuyanyikima i-spacer. Kukwenza oko ngokuhambisa amandla okungapheliyo ngokuchasene neglasi yeglasi kunye ne-aluminium cap. Ngexesha lesampulu, i-screw cap itywina kakuhle. Nayo ingabamba i-septum. Akukho zixhobo zifunekayo kwiNdibano. I-screw cap ine-ptfe \ / isiliki septum. Iqhotyoshelwe kwi-Polyproplene Cap PAYSP usebenzisa inkqubo yasimahla yeSolvent. Le teknoloji yokuxhobisa igcina i-septum kunye ne-cap kunye ngexesha lokuhambisa. Ikwabagcina kunye xa i-cap iqhotyoshelwe kwi-vial. Oku kuqunjelwa i-septum yokukhululeka ngexesha lokusetyenziswa. Kodwa, eyona ndlela iphambili ngayo ingamandla xa uqinisekisa i-cap kwi-vial. I-cap qinisa ukwenza itywina. Ikwaphethe i-septum endaweni ngexesha lokufakwa kwenaliti. Sukuqinisa i-vial cap kakhulu. Iya kwenzakalisa itywina kwaye lenze i-septum iwele. Ukuba i-cap iqinile ngokuqinileyo, i-septum iya kwenza ikomityi okanye indenzelwe indebe.

I-3. I-Snap i-cap cap

II-Snap caplokongezwa kwendlela yokutywina yekhava yomhlathi. I-cap iplastiki. Ilingana ngaphezulu kwe-vim ye-vim. Itywina ngokuncwina i-septum phakathi kweglasi kunye ne-cap yeplastiki eyoluliweyo. Ingxwabangxwaba yeplastiki ibangelwa kukuzama kwayo ukubuyela kubungakanani bayo boqobo. Olu xinzelelo ludala itywina phakathi kweglasi, cap kunye ne-septum. I-snap yeplastiki yeplastiki ngaphandle kwezixhobo.

a. Iziphumo zokutywina kwekepusi ye-snap ayilunganga njengezinye iindlela ezimbini zokutywina.

b. Ukuba i-cap ye-cap iqinile, i-cap iya kuba nzima ukuvala kwaye inokuphula.

c. Ukuba ikhululekile kakhulu, itywina aliyi kuba luncedo kwaye i-septum ingaphuma endaweni.

Amatywina e-vial angcono kunebhotile. Irabha okanye i-silicone iphinde itywine kakuhle emva kokuba ihlawulwe. Zilungele ukugcina i-regegents.

Nangona kunjalo, ezinye izinyibilikisi ziya kulungisa ijeli ye-silica igesi okanye i-gesibes ngexesha lokusetyenziswa. Kule meko, i-vials ifuna iSepta nge-ptfe. Ukuba ngexesha lokusetyenziswa, ukuqeshisa akusetyenziswanga ngaxeshanye, kodwa inxenye yayo ishiywe kwimodeli i-vial vial ngexesha elizayo. Ukuba usebenzisa isampulu kwakhona, oku kwenzeka ngenxa ye-Septa ehlatywe yi-PTFE-ye-PTFE-ye-PTFE elahlekelwe litywina layo.

Irabha okanye i-silicone vial seppas ayinyangeki kwezinye izinto. Ezona bhotile zintle zokuzigcina kuzo zezona zine-crmp tops. I-Retraving Regegents, nangona kunjalo, iirejista ezinobungqina akufuneki zitywinwe kwiibhotile ezikhutshiweyo. I-Rubber isebenza ngcono kune-silicone malunga nokutywina.

Ngaba ungaqinisekanga ukuba loluphi uhlobo lwe-vail olungcono kwiimfuno zakho zelebhu? Jonga inqaku lethu elipheleleyo lokufunda iimpawu ezizodwa kunye nezibonelelo zenketho nganye kunye nokwenza isigqibo: I-crmp vial vs i-vial vial vs.cy cap vial: indlela yokukhetha?

Ngamafutshane, iibhotile ze-HLPC Reagent zibalulekile. Bagcina iisampulu ezicocekileyo kwaye baqinisekisa iziphumo ezichanekileyo. Ukukhetha uhlobo lwebhotile ye-regent ye-regent, ubungakanani, kunye nezinto ezinokwandisa iziphumo zohlalutyo lwakho lwe-HPPC. Ingawunciphisa umngcipheko weempazamo. Isenokuba yi-vial ecacileyo yeglasi yohlalutyo ngokubanzi okanye i-amber enye yeesampulu ezibuthathaka. Okanye, inokuba yinto ekhethekileyo ye-vial yevolumu encinci okanye izicelo zangaphambi kwesilayidi. I-vials elungileyo ye-HPLC inokunceda nawuphi na umsebenzi ohlalutyo uphumelele.

Ngaphambili:

I-vialsgraphy yeChromatography: Iziphumo zokusetyenziswa ixesha elide

Okulandelayo:

Isampulu ye-HPLC ye-vial