I-chialsgraphy ye-chromatographysisixhobo esibalulekileyo sokugcina kunye nokuhlalutya iisampulu kwilabhoratri yohlalutyo. Nangona kunjalo, enye yeengxaki eziqhelekileyo abaphandi zihlala zidibana nazo iibhloksi ezibangelwe ngumcimbi wamathamsali akhoyo kwisampulu. La masuntswana anokuvela kwimithombo eyahlukeneyo, kubandakanya inkqubo yokulungiselela isampulu, ungcoliseko lwendalo kunye nezilwanyana ngokwazo. Ukuqonda oonobangela kunye nezisombululo kwezi bhloso kubalulekile ukugcina ingqibelelo yohlalutyo lweChromatographic.

Umcimbi wamathamsali kwisampulu inokubangela iibhloksi kwi-vial yeChromatographic, enokuchaphazela ukuchaneka kunye nokuzala kweziphumo zohlalutyo. La masuntswana anokuphazamisa inaliti yesampulu, ebangela ukuguquguquka koxinzelelo kwi-chromatographic kwinkqubo ye-chromatographic kwaye unokwenzakalisa izixhobo ezinobuthathaka. Kubalulekile ke ukulungisa le ngxaki.

Kukho izicwangciso ezili-7 zokuthintela kunye nokunciphisa iibhloksi ezibangelwe ngumcimbi wamathambisi kwi-vialsgraphy.

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Ukufakelwa kwesampula kukho inyathelo elibalulekileyo ekuthinteleni iibhloksi kwi-vialsgraphy yeChromatography. Phambi kwenaliti kwinkqubo yeChromatographic, isampulu idluliselwa kwi-filter ukususa umcimbi wokubambisa. Iifilitha zesirinji ziqhele ukusetyenziswa le njongo kwaye ziyafumaneka kwiintlobo ezahlukeneyo ze-pore ukulungiselela iintlobo ezahlukeneyo zesampulu. Iifilitha ze-membrane ezinje nge-nylon okanye i-membrane ye-ptfene ikwasebenza neemfuno eziqinisekileyo zokuhlola. Ukukhetha ubungakanani be-filters efanelekileyo kuqinisekisa ukuba kuphela amasuntswana angaphezulu komgaqo othile osayiniweyo agcinwa, anciphise umngcipheko we-vial block.

Funda ukuba ukhetha njani phakathi kwe-crmp vial vial, i-snap vial, kunye ne-screw cap vial kweli nqaku !:I-crmp vial vs i-vial vial vs.crew cap vial, njani?

Ukuphatha imeko efanelekileyo:

Ukuphatha kwesandla ngokufanelekileyo kubalulekile ukunciphisa ukwazisa nge-vial ye-chial vial. Oku kubandakanya ukuphepha ukungcungcutheka kakhulu okanye ukuphazamiseka ngexesha lokulungiselela isampulu, njengoko ukuxuba okunamandla kunokubangela ukuba amasuntswana akhutshwe kwisitya okanye isampulu ye-matrix. Ukusetyenziswa kwezixhobo ezicocekileyo, i-Intert ezinje ngemibhobho yeglasi kunye neevils ziya kunceda ukugcina imeko icocekile kwaye kunciphise ukubakho kongcoliseko. Ukongeza, ukugcina iisampulu kwindawo elawulwayo engenaluthuli kunye namanye amasuntswana ahamba phambili anciphisa ngakumbi umngcipheko weebhloksi.

Uvavanyo lweVal:

Ngaphambi kokusebenzisaI-chialsgraphy ye-chromatography, Uvavanyo olubonakalayo olucokisekileyo lubalulekile ekufumaneni iimpawu ezibonakalayo zongcoliseko okanye uthatha inxaxheba. Vavanya i-vial vial phantsi kweemeko ezifanelekileyo zokukhanyisa ukubonisa amasuntswana, i-sediment okanye ezinye izinto ezingaqhelekanga. I-Vials ebonisa iimpawu zongcoliseko kufuneka ilahlwe okanye icocwe ngokupheleleyo ngaphambi kokusetyenziswa. I-vials ezibonakalayo ziququzelela uhlolo kwaye ziqinisekise ukuba kuphela ii-vials ezicocekileyo nezingenziweyo zisetyenziselwa ukugcinwa kwesampulu kunye nohlalutyo.

UKUSETYENZISWA KWE-VIELG EIPRINGS ephezulu:

Ukunciphisa iingxaki zokudakumba, kubaluleke kakhulu ukukhetha i-vials yomgangatho ophezulu we-chromatography. Ii-vials zenziwe kwizinto ezifana neglasi ye-borossilicate zibonelela ngokuchasene ne-chimical torances kunye nokukhutshwa kwethafa eliphantsi, ukunciphisa amathuba okungcola okanye ukuphatha inxaxheba kungena kwisampulu. Ngenye indlela, i-vialmer ye-polymer eyenziwe ngezinto ezinje ngePolypropylene okanye i-PTFO ilungele izicelo ezithile ezifuna izikhongozeli ze-InErt kunye nezingasebenzi. Ukukhetha i-vials eqinisekisiweyo kunye nendlela yohlalutyo iqinisekisa ukusebenza ngokuthembekileyo kunye nengqibelelo yesampulu.

Ulondolozo lweNkqubo rhoqo:

Ukuthintela ukudakumba kunye nokuqinisekisa ukusebenza kakuhle, kubalulekile ukwenza ishedyuli ebanzi yolondolozo lwenkqubo yakho yeChromatography. Ukucocwa rhoqo kunye nokugungxula ii-vials, izixhobo kunye nokuthambisa zisusa amasuntswana kunye neentsalela ezinokubangela iibhloko. Sebenzisa iiarhente zokucoca kunye neenkqubo ezicetyiswa ngumvelisi wezinto izixhobo zokuthintela ukonakala kunye nongcoliseko. Iitsheki zenkqubo rhoqo, ezinjengokuvavanywa koxinzelelo kunye nokufumana okuvuyiweyo, kuya kuphucula ngakumbi ukuthembeka kwenkqubo kunye nobomi benkonzo.

Jonga i-vial vial vial caps kunye ne-septa ngokweenkcukacha kweli nqaku! : Ye-vial vial vial caps kunye neSepta, kufuneka uyazi

Uhlalutyo lwe-suricle

Ukuqhutywa kohlalutyo lobungakanani besampulu lubonelela ngengqondo exabisekileyo kwindalo kunye nokusasazwa komcimbi wamanzi. Iindlela ezifana ne-laser ye-laser kunye ne-microscopy zingasetyenziselwa ukuvelisa ubungakanani bobungakanani bokuhanjiswa kwezinto kunye nokuchonga ubungakanani obuphakathi kunye nemithombo yongcoliseko. Olu lwazi lunceda ekukhetheni indlela efanelekileyo yokuqulunqa kunye nezinto ezibonakalayo kwiimfuno ezithile zesampulu, ngokufanelekileyo ukunciphisa umngcipheko weebhloksi ngexesha lohlalutyo.

Intsebenziswano nabathengisi:

Ukusebenza nabathengisi be-vial kunye nezolimo kuqhubela phambili ukufikelela kubungcali kunye nezixhobo zokujongana nemicimbi yokuvala. Abathengisi banokucebisa ukubaluleka kwe-Vial efanelekileyo, iintlobo zeFilters kunye neefomathi zokugcina ngokubhekisele kwiimfuno zakho zeLebhu kunye neempawu zesampulu. Banokubonelela ngoqeqesho lokuphatha ngokufanelekileyo nokugcinwa kokuphucula ukusebenza kwe-vial kunye nokunciphisa ukuphazamiseka okunxulumene nokuhambelana. Ukuseka ubudlelwane bakho

Ngokuphumeza ezi zicwangciso, ilabhoratri inokujongana kakuhle iibhloko ezibangelwe ngumcimbi wamanzi kwivials vialskunye nokuqinisekisa iziphumo ezinokuthenjwa nezingaguqukiyo. Ukongeza kwisandla esifanelekileyo sokuphatha kunye nokulungiswa kwesixhobo, amanyathelo asebenzayo aphambili ukwenza umsebenzi wokusebenza kwe-chromatographic kunye nomgangatho wedatha.

Fumanisa iimpendulo ezingama-50 malunga nee-vials ze-HPLC kule nqaku libanzi !: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Imiba yokuhambelana kwizixhobo ze-chicromatography

Okulandelayo:

Ukujongana nomonakalo wecebo lokucoca iSirilee ngexesha lokusetyenziswa