I-chromatography ephezulu ye-chromatography (i-HPPC) yindlela yokuhlalutywa kwengqondo esetyenziswayo esetyenziselwa ukuqeqeshwa kwezifundo ezahlukeneyo zenzululwazi, ukusuka kumachiza kunye ne-chemistry ukuya kwindalo esweni. Ukuchaneka kunye nokunyaniseka kuxhomekeke kwizinto ezininzi; umntu uhlala etyhafile kodwa into ephambili kukukhetha i-vials yakho ye-HLPC; Oku kudlala indima ebalulekileyo ekupheliseni izikhongozelo kunye nohlalutyo, ukhetha ukufaka i-promatography ophumeleleyo kwi-chromatography - eli nqaku libonisa ukuqwalaselwaFaka i-vials ye-HPLC.

Ukuqonda imisebenzi yokuthengisa

Ukufakwa kukho izinto ezincinci ze-cylindrical ezibekwe ngaphakathi kwi-HPLC Vials ukusebenza ngeenjongo ezibalulekileyo:

I-1. Ukuqulatha iisampulu:Ukufakwa kulungele ukuqulatha iisampulu ezinqongopheleyo nezixabisekileyo, ukunceda ukunciphisa ivolumu yabo ngelixa usabonelela ngokhuseleko olufanelekileyo.

I-2. Ukunciphisa ivolumu efileyo:Ukufakwa kunganceda ukunciphisa ivolumu efileyo kwi-vials, enokuphelisa ukuchaneka kwenaliti kunye nohlalutyo.

I-3. Ukuthintelwa kwesampula:Ukufakwa kunganceda ekuqinisekiseni uhlalutyo oluchanekileyo ngokunciphisa umda kwi-vial kwaye ke nceda ukuthintela isampula. Benza oko ngokuthintela indawo yalo yokuvula ngaphakathi, ngaloo ndlela begcina amathontsi e-racecting ye-adge yenkqubo yokuhlalisa.

4. Ukukhetha:Ukufakwa kunokwakhiwa kwizinto ezahlukeneyo ukuqinisekisa ukuhambelana neesampulu ezahlukeneyo kunye nezinyibilikisi.

Iingqwalaselo ezi-6 eziphambili xa ukhetha ukufaka

Ukukhetha i-vial vial vial vial vial ehlangabezana nazo zonke iimfuno zakho zibalulekile kwiziphumo ezichanekileyo nezinokuthenjwa. Nazi izinto ezimbalwa ekufuneka uzigcine engqondweni:

1. Ithamsaneli yesampula:Ukugcina ngokufanelekileyo iisampulu, qala chaza ivolumu yabo. Ukufaka kuza ngobukhulu obahlukeneyo obuyilungiselela imiqulu eyahlukeneyo yesampulu; Ukukhetha ubungakanani be-subsally ngokufanelekileyo kuya kukuthintela ekuchitheni iisampulu ezixabisekileyo okanye ukugcwala ngaphezulu kwe-vial.

2. Izinto ezibonakalayo:Cinga zombini isampulu yakho kunye ne-selvent ngaphambi kokukhetha izixhobo zokufaka. Ukufakwa kunokwakhiwa ngaphandle kweglasi, i-polypropylene, okanye ipolytetrafluothyyyyyyyyyyyyyyyeon (PTF), phakathi kwezinye; Qinisekisa ukuba iya kusebenza kunye nokuba naluphi na unxibelelwano alubangeli iziphumo ezingafunekiyo.

3. Uhlobo lohlalutyo:Uhlobo lwakho lohlalutyo lunendima enempembelelo yokudlala xa ukhetha izixhobo zokufaka. Umzekelo, uhlalutyo oluphezulu oluphezulu lunokufuna ukukhetha iglasi yokufaka ukunciphisa intsebenzo yesandla kunye nokuqinisekisa ubunyulu obukhulu.

I-4. Ukunciphisa ivolumu efileyo:Ukufakwa kweza kubuqhetseba olwahlukeneyo, njengokufakwa kwengqondo kunye neflethi. I-controcias yokufakwa zihlala zinciphise i-volumu efileyo ngelixa iphucula isampulu yokubuyisela ngexesha.

5. Ukutsala kwetywina:Kwiziphumo eziphezulu, qinisekisa ukuba uyilo lokufaka lihambelana nenkqubo ye-Vial Tokola ukunqanda ukuvuza okanye imicimbi yokungcola.

6. IKhuthalo yesampulu:Khetha ifreyinti ekhusela ingqibelelo yesampulu yakho, umzekelo, ukuba i-thesthereration iya kuba bubulumko ukukhetha itywina elinye ngetywina lomoya.

Ukuqukumbela

Ukukhetha iI-HPPC vial vialOko kudibana nazo zonke iimfuno zakho zohlalutyo kubalulekile ekuqinisekiseni uhlalutyo oluchanekileyo nolinokuthenjwa. Ngokuthathela ingqalelo ngononophelo njengevolumu yesampula, ucaphucalulo lwezinto ezibonakalayo, ukunciphisa ivolusi kunye nesampulu yesampulu yokufumana ulungiselelo lwesampulu kunye nenaliti yeziphumo ezinokuthenjwa ze-HPLC. Sukungahoyi into yokubaluleka; Nokuba izinto ezincinci zibonakala zincinci ezinje ngezi zinto zinokwenza ingxelo ebaluleke kakhulu malunga nomgangatho wedatha eveliswe kuhlalutyo lwe-HLPC.

Ngaba unomdla kwi-HPPC vial vial vial? Sukukhumbula eli nqaku lifundisayo:Ukufakwa kwe-HPLC: ukuphucula inyani kunye nempazamo yesampulu

Ngaphambili:

Iziphumo zevolumu ye-Vial kwi-Cassations

Okulandelayo:

Yintoni i-cap yangaphambi kwe-wake?