I-Chromatography yindlela yokuhlalutya igalelo elisetyenziswa kwiindlela ezahlukeneyo zenzululwazi ezifana ne-chemistry, i-biochemists, kunye nesayensi yendalo. Idlala indima ebalulekileyo ekwahluleni, ichonge, kunye nezinto ezingena kwimixube; Enye into efutho ngayo iziphumo zayo zohlalutyo zinokuba yiyo I-VOUT isetyenziselwa ukubamba iisampulu; Kweli nqaku siza kuphanda le nto ibalulekileyo yokuqhuba uhlalutyo lweChromatography.

Ukuqonda i-chromatography

I-chromatography yindlela yelebhu yokwahlulahlula kunye nokuhlaziya imixube. Isebenzisa izigaba ezibini, iSigaba seSoftware (esinokuba sesolulo okanye igesi) kunye nesigaba sobume (sihlala siqinile okanye sihlalutye nawuphi na umxube ofundwayo. Xa uxutywe kwinkqubo, izinto ezahlukeneyo zenza iSigaba sokuShiti kunye neSigaba seSoftware ngokwahlukileyo kwaye ihamba kwiindawo ezahlukeneyo ezikhokelela kwisando.

Umthamo we-Vial kunye nohlalutyo lweChromatography

Iivils ezisetyenziselwa uhlalutyo lweChromatographic zinokuba nefuthe elikhulu kwiziphumo. Ipharamitha enye ebalulekileyo ukuze ihlale ikhumbula xa ujonge amanqanaba oqeqeshoI-VOUT. Nantsi indlela ekunokwenzeka ngayo ukuhlalutywa kwesampula:

Isampulu yesampula:Ukonyuka kwe-vila ivolumu yoxinzelelo olulinganayo ukuze kuchithe kancinci, kukhokelela kwimogaqo yolwelo njengamanqanaba oxinzelelo ukwehla kwi-vial. Ukuba imilinganiselo echanekileyo yokuxilonga ibalulekile kuhlalutyo, udidi olunjalo lunokutsala iziphumo kwaye lutshintshe iziphumo.

Imida yokufumanisa:Uhlalutyo lweChromatography lufuna imiqobo ebonakalayo xa ujongene nohlalutyo okanye iisampulu eziphantsi zoxinaniso, ezinje ngohlalutyo lwetrayi okanye iisampulu zoxinzelelo eziphantsi. Ivolumu encinci ye-Vial inokuthi incede ukufezekisa imiqobo ephantsi yokuqatshelwa njengoko uxinzelelo lweesampulu luya kuba lukhulu xa iqulethwe ngaphakathi kwezithuba ezincinci.

Imvakalelo:Iindlela ze-chromatographic zihlala zilungelelanisa ubuntununtunu bokuhlaziya; I-vols encinci ye-Vial ihlala inyusa le mvakalelo, yenze kube lula ukuyifumana kwaye iqalise intabalala eninzi eninzi.

USETYENZISIWEYO NGOKUSETYENZISWA:Sebenzisa i-vial vols ifuna ukusonjululwa okuncinci kuhlalutyo, olunokusindisa zombini imali kwaye libe yindawo esingqongileyo.

Iziphumo zeMatrix:Kwiisampulu ezintsonkothileyo, usebenzisa imiqulu encinci ye-Vial inokunceda ukunciphisa iziphumo zeMatrix. Iziphumo zeMatrix zenzeka xa ezinye izihlanganisi kwi-matrix yesampulu iphazamisa uhlalutyo lohlalutyo ekuhlalutyiweyo; Ngokuncipha le mpembelelo yevolumu incitshisiwe kwezi zinto ziphazamisayo.

Ngaba unomdla wokufumana iimpendulo ezizinzileyo malunga ne-vials ye-HPLC? Dive kweli nqaku leight ezinzulu kakhulu:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Iziphumo kwi-Peak SEAMS kunye nesisombululo

Unxibelelwano phakathi kwesampulu yesampulu kunye nenqanaba leselula lidlala indima ebalulekileyo ekuqinisekiseni ubume be-peak. Xa usebenzisa i-Solvent yesampulu kunye namandla aphezulu okwenza isigaba seselula, iincopho zinokubonakala zibangelwa kukobangelwa ngokukhawuleza kwe-Cander-kunokuba kulindelwe. Esi siphumo sinokuba senza sibe senza i-Inamenti yejeyi, ikhokelela kwizigqibo ezigwenxa kunye neencopho ezibukhali ezibukhali.

Kwelinye icala, ukusebenzisa i-Solvent yesampulu kunye namandla aphantsi okuphumelela kusenokuphucula ubume be-pia ngokucinezela kwikholamu, ekhokelela ekwenzeni ukwahlulwa kwabahlalutyi. Eli nqaku ligxininisa ukubaluleka kungekuphela nje komthamo we-vila kodwa kukwakhe nokukhetha isinyibilikisi esetyenzisiweyo kunye nayo.

Ukuqwalaselwa okusebenzayo

IncinciI-VOUTinokubonelela ngemvakalelo enkulu kwaye inciphise ukuphazamiseka; Nangona kunjalo, ukuqwalaselwa okusebenzayo kufuneka kuthathelwe ingqalelo. Ithamo yesampulu kufuneka ihlangane neemfuno zokuhlaziya; I-vostors ezincinci azinakuhlala zilungile.

Ngamanye amaxesha abaphandi bakukhetha ukugxila iisampulu zabo ngaphambi kohlalutyo, bebenza ukuba basebenzise imithanga encinci ye-Vial ngaphandle kokubeka emngciphekweni ingqibelelo yesampulu.

Ukuqukumbela

Ithamu e-Vial yingqwalaselo ephambili kuhlalutyo lweChromatography kwaye kufuneka ikhethwe ngononophelo ukufezekisa iziphumo ezichanekileyo nezinokuthenjwa. Abaphandi kunye nabahlaziyi kufuneka bakhethe ivolumu efanelekileyo ye-vial ehlangabezana neemfuno zabo zokuhlalutya - ukwenza imiqobo ebonakalayo, ngokwemilinganiselo ye-matrix, iimfuno zovakalelo, njl njl.

Ujonge ukukhangela ngakumbi? Dive kweli nqaku ngezicelo ezili-15 ze-vialsgraphys:I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

Ngaphambili:

Izicelo ze-vialsgraphys vials kwi-oyile kunye nohlalutyo lwegesi

Okulandelayo:

Ndikhetha njani ilungelo lokufaka kwi-vial yam ye-HPPL?