Ukuchaneka kwe-chemistry yohlalutyo kubaluleke kakhulu, ngokuchanekileyo kuxhomekeke ekuqulunqweni kwesampulu yesampulu ngaphakathiI-chialsgraphy ye-chromatography. Nokuba ingaba isebenzisa i-chromatography ye-chromatography ephezulu (i-HPPC) okanye i-chromatography yegesi (GC), ukunciphisa ivolumu yesampulu inokuvelisa iziphumo ezinokuthenjwa kunye nohlalutyo lweendleko; Nasi isikhokelo esibanzi sendlela yokubetha le mali icwebekileyo.

1. Yandisa ubuchule bokuphelisa:

Iindlela eziqeshisayo ezinjengenaliti eyahlula-hlula kwi-GC kunye ne-Loop ye-Loop yokugcwalisa i-HPPC inokunciphisa isampulu efunekayo, ngelixa igcina ingqibelelo ngelixa igcina iisampulu ezixabisekileyo.

I-2. Ukufakwa ngokuchanekileyo:

Itekhnoloji yokugcina imibhobho yale mihla ixhotyiswe ngeengcebiso eziphantsi zokugcina ukuqinisekisa ukulahleka komncinci ngexesha lokudluliselwa, ukufuna ukulungelelanisa kunye nobuchule obufanelekileyo ukuze bahambise uluhlu oluchanekileyo lweMicroscole.

I-3. Iindlela zoxinaniso:

Apho kunokwenzeka, uxinzelelo kufuneka lwenzeke ngaphambi kokuba inaliti yesampulu yakho. I-SPME okanye iindlela zokutyikitywa zeSolvent zinganceda ekufezekiseni uxinzelelo oluphezulu kwaye uqinisekise uhlalutyo oluchanekileyo kunye nemithamo esezantsi yesampula.

4. Umda oswelekileyo:

Khetha i-vials kwaye ifake ivolumu encinci yokuthintela ilahleko yesampulu ngenxa yokunamathela kumphezulu. Cinga ngeendlela ezifanaI-contracicalsokanyeI-microvolume vialsnjengezisombululo ezinokubakho.

Jonga ulwazi olunzulu malunga nokufakwa kwe-HPPL kwili nqaku. Fumana ulwazi oluxabisekileyo kunye nolwazi malunga nesihloko esibalulekileyo: Ukufakwa kwe-HPLC: ukuphucula inyani kunye nempazamo yesampulu

5. Qinela i-vialscale ye-microscale:

Ii-vials ze-Microscale zisenziwa ngokukodwa ukuphatha imithamo ye-Ultra-encinci ngaphandle kokungena ngokugqithileyo, ukubonelela ngokuhambelana ngakumbi neendlela zohlalutyo zanamhlanje kunye nokunceda uhlalutyo olusebenzayo.

I-6. I-dilution ngononophelo:

Ngelixa i-dilution yobuchule inokubonakala ngathi iyinyani, inokunceda ukuqhuba uhlalutyo oluchanekileyo. Kodwa oku kufuneka kwenziwe ngononophelo ukuphepha iziphumo ze-dilutions kunye neziphumo ezilahliweyo.

I-7. Ii-amosamplers zangoku:

Itekhnoloji yanamhlanje yanamhlanje ye-Autosamplers yenza ukuba inaliti echanekileyo ye-volus encinci kakhulu, enokusebenza kakhulu kakhulu imizamo yolondolozo. Ukusebenzisa ezo nkalo kuyakunceda imizamo yakho.

8. Ukusebenza kwekholam:

Iikholamu ezigcinwe kakuhle ezisebenza kwi-Peak ukusebenza kakuhle zinokuphucula ukwahlukana, ukuvunywa kohlalutyo lweesampulu ezincinci zesampulu ngaphandle kokusebenzisa umgangatho.

9. Nciphisa ungcoliseko lwesampula:

Ungcoliseko ovela kwi-Vial vial vial vial vial vil vil vil vil inokuthi ifumane iziphumo, ke qhelisela ubuchwephesha be-lab efanelekileyo kwaye uphatha ii-vials ngononophelo ukuze kuncitshiswe ungcoliseko.

I-10. Uqinisekiso kunye noLwabisayo:

Qinisekisa rhoqo kwaye ucoce izixhobo zakho ukuze kuqinisekiswe ukuba ubuchwephesha bokunciphisa ivoramu yesampula bavelisa iziphumo ezichanekileyo.

Ngomhla wokudakumba onzima we-chromatography, ibhalansi phakathi kokuchaneka kunye nokugcina iisampulu ezixabisekileyo kunokufezekiswa ngezenzo ezicinga. Ukusebenzisa iindlela ezifanelekileyo zokuphelisa, itekhnoloji ye-Advance, kunye nobuchule be-Meticulous zinokuchaphazela uhlalutyo lwakho ukuya kwiindawo eziphakamileyo. Njengoko uluntu lwenzululwazi luyaqhubeka ukutyhala imida yoko kunokwenzeka, ubugcisa bokunciphisa ivoramu kwi-chicromatography iba nobuchule obubalulekileyo bophando oluchanekileyo nolusebenzayo.

Fumanisa ulwazi olupheleleyo kwi-50 yemibuzo ebuzwa rhoqo malunga ne-HPLC Vials kwinqaku elinolwazi. Vula iimpendulo ozifunayo: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Ungazicoca njani ngokufanelekileyo i-HPLC vial vial & trays? Amanyathelo ayi-16 aneenkcukacha

Okulandelayo:

Ndikhetha njani i-septum elungileyo ye-vial ye-HPPL yam?