I-chromatography, indlela esisiseko yohlalutyo, isetyenziswa kwimimandla eyahlukeneyo yesayensi yohlalutyo lweesampula ezichanekileyo. Ukuchaneka kweziphumo ze-chromatographic kuxhomekeke kumgangatho kunye nokunyaniseka kwesampulu egciniweyo. Enye yezona zinto zibalulekileyo ekugcineni le ntembeko kukugcina ngendlela efanelekileyo yee-vials zeChromatographic. Esi sikhokelo siqokelela iinkqubo ezisisiseko kwaye zibonelela ngendlela ebanzi yokugcina umgangatho wesampulu ngexesha lokugcina.

1.Khetha i-vial efanelekileyo:

Inyathelo lokuqala lokugcina ingqibelelo yesampulu ibandakanya ukukhetha ngokufanelekileyovial vial vial. Izixhobo ezinje ngeglasi ye-borossilicate okanye i-Intert icetyiswa ukunciphisa intsebenzo phakathi kwesampulu kunye nenqanawa. Cinga ngendlela ethile ye-chromatographic esetyenzisiweyo (igesi yegesi (GC) okanye i-chromatography ye-chromatography (LC) kwaye iqinisekise ukuba i-vials iyahambelana. Ukukhetha i-vials efanelekileyo kubeka isiseko senkqubo yokugcina amandla.

I-vials yeglasi

I-vials yeglasi, ngakumbi ezo zenziwe kwiglasi ye-boroslilicate, zixhathise kakhulu imichiza kwaye zincinci ekuvuseleleni ungcoliseko kwiisampulu, zibenzela ukuba zilungele uhlalutyo olubuthathaka. Iglasi inokumelana namaqondo obushushu aphezulu kuneplastiki, enokuba yingenelo kwizicelo ezithile ezifuna uzinzo lwe-shirmal. I-vials yeglasi ye-Amber iyilelwe ngokukodwa ukukhusela iisampulu ezibuthathaka ukukhanya ukusuka kwi-UV Demodition, qinisekisa uzinzo lwexesha elide.

I-vials yeplastiki

I-vials yeplastiki, ezinjengezo zenziwa kwiPolypropylene, zilula kwaye ziqinile kune-vials yeglasi. Oku kuluncedo kwiindawo apho ukohluka kuyinkxalabo. Ngokubanzi, i-vials yeplastiki ayibiza kakhulu kune-vials yeglasi, ibenze inyathelo elisebenzayo lokuhlalutya rhoqo apho ukumelana nemichiza akukho mfuneko yexabiso. Ngelixa ezinye iiplastiki zinokuba nazo iikhemikhali ezifanelekileyo, zisenokungabi lilungele zonke iindidi, ngakumbi izinyibilikisi eziguqukayo okanye ezinobundlobongela.

2.Cencleanzuluanziolity Iqhosha:

Kubalulekile ukuqinisekisa ukuba zombini ii-vials kunye ne-CAPS zicocekile ngokugqibeleleyo ngaphambi kokugcina iisampulu. Nakuphi na ukungcola okanye ukungcola okushiyekileyo kunokuphawula ingqibelelo yesampulu. Hlamba i-vials kunye ne-CAPS ngesisombululo esifanelekileyo sokucoca, silandelwe ngokucocekileyo nge-solvent ecocekileyo ephezulu yokususa isixa semizuzu yokungcola. Inkqubo yokucoca icocekileyo ilungiselela isampulu egciniweyo yokugcina ubunyulu bayo.

Ngaba unomdla wokuqonda ulwazi olupheleleyo lokucoca ii-vialsgraphy ze-vialsgraphy? Dive kwinqaku lethu ukufumana ulwazi olupheleleyo kwiindlela zokucoca ezifanelekileyo. Vula iimfihlo ngoku!Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys

3. Ukutywinwa kweProperper:

Ukugcina itywina le-airtight kubalulekile ukuthintela i-Ingress yomoya kunye nokufuma, okunokuchaphazela kakubi uzinzo lwesampulu. Qinisa i-caps ngokukhuselekileyo ukwenza itywina le-airtight kwaye uqwalasele usebenzisa ii-sepgus okanye ii-liners ukongeza ukutywinwa. Khetha i-caps ye-Inert ye-Inert ukunqanda ukusebenzisana ngesampulu. I-Vial etywiniweyo eqinileyo iqinisekisa umgangatho wexesha elide wesampulu ngexesha lokugcina.

I

Ulawulo lobushushu yinto ebalulekileyo ekugcineni uzinzo lwesampulu.I-chialsgraphy ye-chromatographykufuneka igcinwe kwindawo elawulwa ngobushushu (ihlala ibanda) ukulibazisa ukuphendula kweekhemikhali kunye nokunciphisa umngcipheko wesampulu. Iifriji okanye i-freezers zilungele iisampulu ezininzi, kodwa kubalulekile ukuthelekisa amaqondo obushushu agcina iimpawu ezithile zesampulu.

Gcina i-vials kwiqondo lokushisa rhoqo, liphakathi kwe---20 ° C kunye ne-4 ° C, kuxhomekeke kuhlobo lwesampula. Kulumkele ukutshintsha okukhawulezayo kumaqondo obushushu okuthintela ukwaphuka kweglasi okanye ukonakaliswa kwesampulu. Qinisekisa ukuba indawo yokugcina yomile ukuthintela umswakama ekubeni ichaphazele ingqibelelo yesampulu okanye i-vial.

5. ISIQINISEKISO SOKUGQIBELA:

Ukuvezwa kukukhanya kunokuba nefuthe elibi kwiisampuli, ngakumbi iisampulu ezibuthathaka. Cinga ngokusebenzisa i-Amber okanye i-vials enemibala ukukhusela iisampulu ngaphandle kokukhanya, ngakumbi ukuba ziqulathe iifoto zefoto. Ukugcina i-vills kwi-mnyama okanye kwizitya ze-opaque zibonelela ngomaleko ongezelelweyo wokukhuselwa kwimeko ekuthambekileyo kunye nokuqinisekisa uzinzo lweesampulu ezibuthathaka.

6.Sebenza kunye neelebheli:

Ukugcina indlela ecwangcisiweyo yokulungiselela igcinweI-chialsgraphy ye-chromatographyibalulekile ekubuyiselweni ngokufanelekileyo kunye nokulandela umkhondo. I-vial nganye kufuneka ibhalwe ngokucacileyo ngolwazi olufanelekileyo njengegama lesampulu, umhla wokulungiselela kunye nezinye iinkcukacha ezifanelekileyo. Oku akuqinisekisi kuphela ukulandelela kodwa kukwenza ulawulo olululo lweesampulu ezigciniweyo. Ukuphunyezwa kombutho oqingqiweyo kunye neelebheli zelebheli kuya kulungelelanisa i-Labhoratring yeLebhu kwaye igalelo kwimpumelelo yesayensi.

Ukugcinwa ngendlela efanelekileyo ye-vials yinkqubo eninzi enefuthe elibonakalayo kwingqibelelo yesampulu kwilabhoratri yohlalutyo. Ngokulandela isikhokelo esipheleleyo esichazwe kwesi sikhokelo, abaphandi banokunyusa ukuthembeka kunye nokuchaneka kohlalutyo lweChromatographic. Ukuchitha ixesha kunye nokuqwalaselwa kwinkqubo yokugcina khusela i-teko yesampulu kwaye kunegalelo kwimpumelelo yovavanyo lwesayensi kunye nohlalutyo.

Ndinomdla malunga ne-vials ye-HPLC? Ukutyhila iimpendulo ezingama-50 ezifanelekileyo kwinqaku elipheleleyo. Fumana iinkcukacha ozifunayo ukuze ufumane ukuqonda okungcono kwe-vials ye-HPLC. Jonga ngoku !:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Yonke into oyifunayo ukwazi malunga nokubaluleka kokukhetha i-cap ye-chromatographys vials

Okulandelayo:

Iimpawu ezi-8 eziphambili ze-vials zeglasi nge-ptfel ye-ptyl ye-rabber sepga