Isampulu yoqobo ibandakanya yonke imisebenzi ngaphambi kwenaliti. Ngaphandle kokuncipha, ukunyibilikisa, kunye norhatya, kubandakanya la manyathelo alandelayo:

Ukuthoba
Ukukhutshwa
Ukugxothwa
I-chromatography ye-chromatography (iKholamu yoTyco lwe-chromatography)

I-Preymenty inokwenziwa ngesandla okanye ezenzekelayo. Ngaba uqhelene nesampulu yesampulu?

Ukugxothwa

Ezinye iisampulu azisakwazi ngqo emva kokukhulisa. Ukuncitshiswa kuyadingeka ukwenza iikhawuntari ngaphandle kwe-UV

Iphucula ubuntununtunu
Ukuphucula ukwahlulwa (ukutshintsha komgangatho)

Imicimbi enokubakho ibandakanya ilahleko yesampulu, ungcoliseko, ukuphendula okungaphelelanga, okanye iimveliso ezininzi, ezinokuchaphazela ukuchaneka okanye ukwazisa iimpazamo.

Isampulu

Iisampulu ze-LC kufuneka zibe neyunifomu kwaye zikhululekile; Amasuntswana anokonakalisa intsholongwane okanye ibhlokho kwikholamu. Amanyathelo abandakanya:

Shake isampulu ngaphambi kwenaliti kwaye ujonge amasuntswana, i-turbidity, okanye i-emulsions
Ukuba amasuntswana ayaqwalaselwa, hluza isampulu usebenzisa i-membrane engagcina i-≥0.15M

Imicimbi enokubakho: Ungcoliseko lwesampulu, ukunciphisa ukugxininiswa kwento, kunye nokukhutshelwa i-solvent ebangela iimpazamo.

Iindlela zokukhuphela

Ukukhupha eyahlulahlulwa kwiindawo ezinamayeza okanye zisusa izinto eziphazamisayo (i.G., iiproteni). Izinyibilikisi zezinto eziphilayo mazibe ziphantsi kwetyhefu, i-witlaile, iphantsi kokungcola, kwaye ichithe isampulu kakuhle.

Izinyibilikisi eziqhelekileyo: I-Dietyyl Ether, Ethyl Acetate, Dichloromethane, Chloroform, Benzene, okanye umxube.

Emva kokukhutshwa, iisampulu zinokutshitshiswa ngqo okanye zigxile ekunyukeni uhlalutyo kunye nemvakalelo.

Isampulu yesampulu ejijekileyo yamanzi

Amacandelo akwi-acidic kunye nezikhuselo: Ukungcola kwe-organic yeShorvent, lungisa i-acidic, thambisa okanye ilahlekile; okanye yomile phantsi kwe-n₂ kunye nokunyibilika kwi-solvent efanelekileyo ngaphambi kwenaliti.
Izinto ezisisiseko kunye nezikhuselo: Isiphumo sokungcola kweShorganic Solvent, hlengahlengisa kwisiseko, thambisa okanye ilahlekile; okanye yomile phantsi kwe-n₂ kunye nokunyibilika kwi-solvent efanelekileyo ngaphambi kwenaliti.
Amacandelo angathathi hlangothi: Khupha ukungcola kweShorgacntren, emva koko uhlalutshandu ngokuthe ngqo kwi-chromatography yenqanaba lokubuyiswa.

Isampulu ye-Fit

Khupha i-organic slinvent okanye ilahle ngqo; okanye yomile phantsi kwe-n₂ kunye nokunyibilika kwi-solvent efanelekileyo ngaphambi kwenaliti.

Imithombo yongcoliso

Imithombo enokubakho yongcoliseko kuhlalutyo:

Uhlobo lokungcolisa	Umthombo	Amanyathelo okuthintela
Okusingqongileyo	Iigesi eziyingozi, Uthuli, Aerosols	Gcina igumbi lesixhobo sedwa, licocekile; faka i-AC; Ukulawulwa komnyatha <70%; Thintela ukufuma, i-corosion, vibration
Izikhongozeli	Iglasi, i-ceramic, i-quartz, i-labore yeplastiki	Khetha izikhongozeli ezifanelekileyo ngohlobo lwesampulu; Coca ngokufanelekileyo
Iirejista	I-Remegents ye-On-chromatopy	Sebenzisa iBanga le-Chromatography, ukucoceka okuphezulu, okanye ukuhlalutya kwebanga lokuhlalutya

Ukulungiswa kwesisombululo esisezantsi

Lungiselela isicombululo esisezantsi Sebenzisa i-batch efanayo kwimigangatho kunye neesampulu ukunciphisa iimpazamo
Izinto kufuneka zibe yinqanaba le-chromatography kunye nezinzileyo
Gcina izisombululo ezisemgangathweni kwiibhotile ezimdaka kwiqondo lokushisa eliphantsi
Iivithamini, sebenzisa iiflethi zomntu onsundu okanye ukhuselwe ekukhanyeni

Iingxaki eziqhelekileyo kwiSampulanga kunye nezisombululo

Ingxaki	Unobangela	Isisombululo
I-Peaks engadibaniyo kwiChromatogram	Ukucoca ungcoliseko	Isihluzi se-soik kwi-smolvent yesampulu kunye nenaliti yovavanyo Guqula uhlobo lokucoca Sebenzisa ezinye iindlela zokucoca
Impendulo ephantsi ephantsi (ngakumbi iisampulu zoxinzelelo eziphantsi)	I-adsorption kumhlaba wokucoca	Guqula uhlobo lokucoca Qhuba zonke iisampulu phantsi kweemeko ezifanayo Sebenzisa ezinye iindlela zokucoca
Ukuvuselela okuphantsi	Ukukhutshwa okungaphelelanga	Yongeza ixesha lokukhupha, sebenzisa i-slinvent eshushu Lungisa indlela yokucoca
Iincopho ezibandayo, ixesha elifutshane lohlobo lwekholamu	Ukuphazamiseka kwesampulu kunye nongcoliseko	Ukuphucula indlela yokucoca
Ukuchaneka okungalunganga	Ukuchacha okungaphelelanga	Qinisekisa okanye ukutshintsha i-derivatition, ukwahlulwa, ukukhupha, okanye ezinye iimeko Sebenzisa ukuphatha okuzenzekelayo ukuphucula ngokuchanekileyo

Ngaphambili:

Kakhulu \ / ubushushu obuphantsi kunye nefuthe lokubonisa ukukhanya kwisampulu yozinzo: Ithiyori kunye nePhambilo

Okulandelayo:

Buyela kuLuhlu