I-chromatography, indlela ebalulekileyo yemichiza kunye nesayensi yohlalutyo, iqashwe ngokubanzi yokwahlulahlula kunye nokuhlalutya umxube onzima. Ifumana izicelo kumashishini onke, ukusuka kwiindawo zamayeza ukuya kwindalo esingqongileyo kuhlalutyo lokutya. I-vials yesampulu kwaye ifake i-Dlala inxalenye yenxalenye yolu hlobo kwi-gesiives yeglasi ifumene udumo ngenxa yeengenelo zabo ezininzi; Siza kuphonononga ukuba kutheni ukuthandwa kwabo kuyaqhubeka nanamhlanje kweli nqaku.

Ukuqonda i-chromatography

Phambi kokuba ucoceke ngakumbi kwiglasi yeglasi, masihlolisise ngokufutshane iziseko ze-chromatography. I-Chromatography yindlela yokwahlulahlula umxube ngokwabo kwizinto ezibonakalayo kwisigaba sesikhululo samaShiti esiSitimenti seStores - Ngokwesiqhelo okanye ulwelo) kunye nezigaba ezibini eziphathwayo - zihlala zegesi okanye ulwelo. Izinto eziphambili zayo nayiphi na inkqubo yeChromatographic yikholamu, i-vial ye-vial, kwaye ifakwe.

Indima yeGlasi yeGlasi

Iglasi yeglasiNgaba ii-tube zeglasi ezincinci zenzelwe ngokukodwa ukuba zihambelane ngaphakathi kweengcongconi ezisetyenziselwa i-chromatography. Ngelixa kukho ubungakanani obahlukeneyo kunye nemilo yokufakwa kwemidlalo, imilo yabo yeyabo ihlala ingoyena mntu ufuna kakhulu kuzo ngenxa yezibonelelo zayo ezininzi.

Zili-12 izibonelelo zeglasi yequmrhu kwi-chromatography

Iglasi yequmrhu ifaka ukubonelela ngolawulo oluphuculweyo lolawulo:

Enye yezona zibonelelo ziphambili zokufaka iglasi kukufaka amandla abo okulawula isampula. Uyilo lwabo lubandakanya amazantsi anemigca enciphisa indawo yomphezulu eboniswe emoyeni-eli nqaku liluncedo xa lisebenza neesampulu ezincinci okanye inza imijikelezo yevolumu encinci njengoko inceda ekuqinisekiseni ukunyaniseka kwabo ngolo lonke uhlalutyo lweColotoographic.

Ukuphucula ukubuyiswa kwesampula:

Ezi ngxaki zeMiquluns ziqhuba i-Recaction Cam Reaction Capetional emva kohlalutyo. I-Pipette okanye i-Aspirate iisampulu zakho ngqo ukusuka ezantsi kwe-fan yokufaka kwinkunkuma encitshisiweyo xa ujongene neesampulu ezilinganiselweyo okanye ezixabisekileyo. Eli nqaku liluncedo ngakumbi xa usebenza ngeesampulu ezilinganiselweyo ezingenakwenziwa.

Ukuncitshiswa komthamo ofileyo:

Conical glass inserts help minimize dead volume - or the portion of sample remaining within its vial after analysis - by optimizing sample recovery for accurate quantitative chromatography analysis. Uyilo lwabo lukhulula isampula yokubuyiselwa kwesampulu yeziphumo ezichanekileyo.

Ngaba unomdla wokufunda ngakumbi malunga ne-HPPC ye-HPLC? Dive kweli nqaku elifundisayo ngoshwankathelo olubanzi:Ukufakwa kwe-HPLC: ukuphucula inyani kunye nempazamo yesampulu

Ukuhambelana:

Ukufakwa kweglasi ye-comfical kuyahambelana ne-autosamplers kunye neendlela zenaliti ezisetyenziswa kwi-chromatography, kuqinisekisa ukusebenza ngokuthembekileyo kuzo zonke izixhobo kunye nabavelisi. Uyilo lwabo olufanayo luqinisekile ukuba kungenamthungo kufanelekile ukuphunyezwa ngokulula kwizixhobo ezahlukeneyo kunye neempawu.

Ukukhusela isampula:

Ukongeza kokuthintela isampulu yesampulu, iglasi yenkoza ifaka iisampulu ngokhuselo olongezelelekileyo, lokugcina ungcoliseko lungaphandle kunye nokugcina ubunyulu ngexesha lokugcina kunye nohlalutyo.

Uluhlu olubanzi lwezicelo:

Iglasi yeglasiinokufumaneka kuluhlu lweendlela ze-chromatographic, ezinjenge-chromatography yegesi (i-GC), i-chromatography ye-chromatography (i-LC) kunye ne-chromatography ye-chromatography (i-HPPC), ibetha ngokufanelekileyo ukujongana nemiceli mngeni emininzi yohlalutya.

Ulawulo lobushushu:

Uhlalutyo lweChromatography luhlala luxhomekeke kwiisampulu ezinobuthathaka lobushushu, ukwenza iglasi ye-comfic kufaka indlela ebalaseleyo yokulawula ubushushu babo ngexesha lohlalutyo. Ihambelana neenkqubo zokulawula ubushushu, iglasi yeglasi yomdibaniso ivumela ummiselo wobushushu obuchanekileyo ngexesha lokuhlalutya.

Ukuphatha ngokulula:

Ukufakwa kweglasi yomdibaniso kunobuhlobo, njengoko uyilo lwabo olulula luyenza ukuba ingagungqi iphazamise kwaye ilayishwe kwii-vials zesampulu. Ukusetyenziswa kwabo kunciphisa umngcipheko kwiinkqubo zesampulu zesampulu yokuzalwa kokuzala kuvavanyo.

Isebenza kakuhle emalini:

Iglasi yequmrhu ifaka izibonelelo ezininzi ngaphandle kokungenela iindleko ezingeyomfuneko, ukubonelela ngabaphandi ngokuphucula isampulu yokuphathwa kwesampulu kunye nokuhlalutya kwenkqubo ebonakalayo.

Iinketho zokhetho:

Abavelisi abaninzi beglasi yequmrhu ifaka ukubonelela ngeendlela zokwenza ukuba abaphandi banyule ukufaka iimpawu, njengeempawu, ezinjengeemfuno ezikhethekileyo, ezihlangabezana nezicelo zezicelo ezithile ze-chromatographic.

Ukuqwalaselwa kwendalo:

Iglasi yindawo enokuphinda isebenze ngokulula, ke ukhetha iglasi yeglasi ye-meg njengezenzo ze-EC-GOOROORT zenza ukuba kube sengqiqweni kwindalo esingqongileyo. Ukunciphisa inkunkuma yeplastiki enxulumene nokufakwa kokuchithekayo yenye inyathelo lokugcina uzinzo kwilabhoratri.

Isondlo sethemba seSampule:

Iglasi yomdibaniso iqinisekisa ukuqulunqwa kunye neepropathi zeesampulu ezibuthathaka zihlala ziphazamisekile ngexesha lokugcina kunye nohlalutyo lweziphumo ezichanekileyo nezinokuthenjwa. Oku kubalulekile ukufezekisa uhlalutyo oluchanekileyo nolukubi ngexesha elifanelekileyo.

Ukuqukumbela

KwiChromatography,Iglasi yeglasiziziqondakalise izixhobo ezingabonakaliyo ngenxa yokukwazi kwabo ukuphucula ulawulo lwesampulu, ukubuyisela kunye nokukhusela. Abaphandi kunye nabahlalutyi kumashishini ahlukeneyo banokuzisebenzisa ngenxa yokusebenza kwabo, ukungqinelana nokulula kokusetyenziswa; Ukuzenzela izinto ezingabonakaliyo kwiindlela zeCromatographia zanamhlanje. Njengoko imiceli mingeni yohlalutya iyaqhubeka ukwanda kobunzima, iglasi yomkhondo ifaka izixhobo ezinokuthenjwa eziqinisekisa iziphumo ezichanekileyo zesayensi.

Ngaba ujonge iimpendulo ezibanzi malunga ne-vials ye-HPLC? Jonganga phaya-jonga eli nqaku kuzo zonke iinkcukacha ozifunayo:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

I-conunterum yendleko: I-chromapys yexabiso le-vials vs. I-vials yepremiyamu

Okulandelayo:

Isikhokelo esipheleleyo kwii-0,45 ze-micron filters: yonke into ofuna ukuyazi