I-chromatography, ubuchule obusisiseko kuhlalutyo lwe-chemistry, buxhomekeke ekuchanekeni nasekuthembekeni kolwahlulo oluchanekileyo kunye nohlalutyo lomxube obanzi. Phakathi kwezinto ze-miyad kubalulekile kule nkqubo, i-polytetrafluoroothylene (PTFE) kunye neSilicone Septums Dlala indima enkulu. Eli nqaku lihlola izizathu ezinyanzelekileyo emva kokusetyenziswa ngokubanzi kwePtfe \ / isiliki seiclemskwi-chromatography.

I-Inert ye-Inert-esisiseko:

I-Polytetrafluoroothylene Le ndawo ibaluleke kakhulu kwi-chromatography, apho usukelo kukuhlisela ngokuchanekileyo kwaye luhlalutya izinto zomxube. I-ptfe septum iqinisekisa ukuba ukusebenzisana okungafunekiyo kwenzeke phakathi kwezinto ze-septum kunye nesampulu engasenziweyo. Le ntsimi yekhemikhali ibaluleke kakhulu kwizicelo apho ingqibelelo yesampulu kufuneka igcinwe, njengoko ukusamkela kunokugqwetha iziphumo. Ukuthembeka kwezinto ze-ptfees kwi-PPURMS ekugcinweni kwesampulu ibalulekile ekufezekiseni iziphumo ezingaguqukiyo nezibuyisiweyo ze-chromatographic.

Ukutshintsha kunye nokungasebenzi:

Indibaniselwano ye-PTFA kunye ne-silicone iphumela kwi-septum enezinto eziguquguqukayo ezibonakalayo kunye nokuhambelana. I-chromatography ibandakanya uluhlu olubanzi lobuchwepheshe, nganye ineemfuno zayo ngokubhekisele kubushushu, uxinzelelo kunye nokuhambelana nezinyibiliki ezahlukeneyo.Ptfe \ / isiliki seiclemsbonisa ukuguquguquka kufuneka kunanini uluhlu lweemeko kwaye zilungele izicelo ezinje nge-chromatography yegesi (i-GC) kunye ne-chromatography ye-chromatography (i-HPPC). Ukuguquguquka kwayo kwenza ukuba kuthathe ukhetho lweelebhu kwiilebhu ezisebenza ngeendlela ezahlukeneyo zokuhlalutya.

Ndinomdla wokukhetha phakathi kwe-pre-slit kunye ne-septa engeyiyo i-sepga? Dive kweli nqaku lokuqonda ngokwenza isigqibo esifanelekileyo seemfuno zakho zeChromatography:Ungayikhetha njani i-Septa Pal-Slit okanye hayi?

Kunciphisa isampulu yongcoliseko:

Imodeli ungcoliseko lwesampulu yinkxalabo eqhubekayo kwi-chromatography kunye ne-ptfe \ / i-silicone seipms ilungisa ngokufanelekileyo lo mngeni. Ezi sexhum zenzelwe ukuqinisekisa amanqanaba asezantsi eLeacha kwaye akhutshiwe, ukunciphisa umngcipheko wesampulu yokuqulunqwa kwesampulu. Ukucoceka kwezinto zeSeptum kubalulekile ekuhlaziyeni kovavanyo, apho usukelo lokufumana kunye nokudibanisa izinto kugxininiso olusezantsi kakhulu. I-ptfe \ / i-Silicone Sepms inegalelo elibonakalayo ekuthembekeni nasekuchanekayo lwedatha yeChromatographic ngokunciphisa ungcoliseko lwesampulu.

Ngaba unomdla wokuphila kwihlabathi le-HPLC ye-vial vial sepga? Jonga ngakumbi - uphonononge iinkcukacha kweli nqaku malunga nokuqonda okuxabisekileyo kunye nolwazi:Yintoni i-vial vial sepga?

Iipropathi ezibalaseleyo zokutywina:

Kwiinkqubo zeChromatography, itywina elikhuselekileyo neluthembekileyo libaluleke kakhulu ukuthintela ukuvuza kweendlela zokuzonwabisa kunye nokugcina ingqibelelo yesampulu. I-ptfe \ / i-silicone sepums i-Excel kwitywina elisebenzayo phakathi kwe-vial kunye ne-port yenaliti. Indibaniselwano yokuqina kwe-ptfe kunye ne-elaistity ye-silicone iqinisekisa itywina eliqinileyo neliqinileyo, nkqu phantsi kweemeko zobushushu kunye noxinzelelo. Le nto yokutywina kwe-hermetic ingakukhuseli isampulu, kodwa inomtsalane obalulekileyo ekuthinteleni unxibelelwano olungalindelekanga olunokulalanisa ngokuchanekileyo kohlalutyo lweChromatographic.

Ukuqina kunye nobomi obude:

Iilebhu ezijongana negalelo eliphezulu lesampulu kufuneka zisebenzise izinto ezingathembekanga kodwa ziqine. I-ptfe \ / i-Silicone Serium yaziwa ngobubi babo kwaye inokumelana nenaliti ezininzi ngaphandle kokunxiba okukhulu okanye ukonakala. Ukuqina kwezi zinto zikhokelela ekusebenzeni kweendleko zelebhu njengoko zinciphisa irejista yotshintsho lwe-septum kunye nokucutha ixesha lokuphumla. Ubomi obude be-PTFO /

Ukuqukumbela, ukusetyenziswa kwePtfe \ / isiliki seiclemsKwi-chromatography isekwe kwiipropathi ezisisiseko ezinjengemikhamikhali yekhemikhali, ukungahambi kakuhle, ukuncitshiswa, ukuncitshiswa kongcoliseko, izinto ezibalaseleyo zokutywina kunye nobomi obude. Ezi sepfum zidlala indima ebalulekileyo ekugcineni ukuchaneka kunye nokuthembeka kohlalutyo lweChromatographic, zibenza ukuba babe yinto ebalulekileyo kwiilebhu zahlalutya kwihlabathi liphela. Njengokuqhubela phambili kwetekhnoloji, ukubaluleka kokunyamezela kwe-PTFA \ / Isilicon seipgus kubandakanya indima yayo ephambili ekwakheni ikamva le-chromatography.

Ndinomdla wokufumana ulwazi olupheleleyo malunga ne-ptfe \ / isiliki septa? Sukuphoswa-jonga eli nqaku lokuqonda okucokisekileyo ukubaluleka kwabo kwi-chromatography: Yonke into oyifunayo

Ngaphambili:

Ukulahla ngokufanelekileyo i-vialsgraphy ye-chialsgraphy? Amanyathelo ama-7

Okulandelayo:

Yonke into oyifunayo ukwazi malunga nokubaluleka kokukhetha i-cap ye-chromatographys vials