I-chialsgraphy ye-chromatographyIsixhobo esibalulekileyo kwilebhu, sincedisa ukwahlukana kunye nohlalutyo lweentambo kwiintlobo ezahlukeneyo zezixhobo. Njengaso nasiphi na isixhobo selebhu, ukulahlwa kwabo kubalulekile ukuqinisekisa ukhuseleko lwabasebenzi belebhu kunye nokukhusela indalo. Esi sikhokelo sibonelela ngamanyathelo athile ukuba iilebhu ezifanele zisuse ukulahla i-chromatography ye-vials.

Malunga ne-vialsgraphy yeChromatography:

I-vialsgraphy yeChromatography zizikhongozeli ezenzelwe ukubamba iisampulu ngexesha lenkqubo yeChromatographic. Zifumaneka ngeendlela ezahlukeneyo, kubandakanya iglasi kunye neplastiki, kunye nobungakanani obuhlukeneyo ukulungiselela imiqulu eyahluka. Ukunyaniseka kohlalutyo lweChromatographic kuxhomekeke kakhulu kumgangatho kunye nokucoceka kwezi vila. Nje ukuba uhlalutyo lugqityiwe, kuyanyanzeleka ukuba ukuchithwa kwabo kuphathwe ngononophelo.

Iindidi ze-vialsgraphys vials kunye nokuqwalaselwa kwenkunkuma yazo

I-vialsgraphy yeChromatography iza ngeendlela ezahlukeneyo, kubandakanya:

I-vials yeglasi: Ihlala isetyenziselwa izicelo ze-HLLC kunye ne-GC. I-vials vials inokuthi iqulethe izinyibilikisi ezikhoyo ezifuna ukulahlwa ngononophelo.

I-vials yeplastiki: Isetyenziselwa izicelo ezithile, ezi vils zinokuba ngaphantsi kwe-chemis enganyangekiyo kuneglasi kwaye inokufuna iindlela ezahlukeneyo zokulahla inkunkuma.

I-Vials ekhethekileyo: Ezi zibandakanya i-vials ezenzelwe izicelo ezithile, ezinje ngohlalutyo lweentloko okanye i-ofisi ye-ofisi ye-Otoot yeSebe (VOC).

Uhlobo lwe-vial olusetyenzisiweyo luya kuba nefuthe kwindlela yokulahla, ngakumbi malunga nezixhobo kunye neziqulatho zentsalela.

Amanyathelo ama-7 okulahlwa ngokuchanekileyo:

Inyathelo 1.Inqanaba le-vials

Kubalulekile ukuqinisekisa ukuba nayiphi na isampulu eseleyo kwi-chicromatography vial ikhutshiwe ngokupheleleyo ngaphambi kokulahlwa. Eli nyathelo alithinteli ukungcola, kodwa kukuqinisekisa ukusebenza ngempumelelo kwenkqubo yolawulo lwenkunkuma elandelayo.

Inyathelo 2.Ukuhombisa

I-Vials enezixhobo eziyingozi okanye eziyityhefu zifuna inkqubo yokubeka i-tocoCamping. Oku kubandakanya ukukrazula i-vial ngesisombululo esifanelekileyo okanye isisombululo sokucoca ukususa nayiphi na imikhondo yentsalela yesampulu kwaye unciphise umngcipheko wemicimbi yendalo esingqongileyo okanye yokhuseleko.

Inyathelo 3.Ukwahlula

Ukwahlulwa kwenkunkuma efanelekileyo kubalulekile ukuba kulawulo lwenkunkuma efanelekileyo. I-vialsgraphy yeChromatography kufuneka idityaniswe enye inkunkuma yelebhu yokulungisa inkqubo yokulahla inkunkuma kwaye ihambelane neeprothokholi zolawulo lwenkunkuma.

Ngaba unomdla wokuxhasa ubugcisa bokucoca ii-vialsgraphy ze-vialsgraphy? Dive kweli nqaku ngesikhokelo esibanzi !:Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys

Inyathelo 4.Ukuphinda kusetyenziswe kwakhona

Nanini na apho kunokwenzeka, iilebhu kufuneka zibeka phambili i-vialsgraphy ye-vialsgraphy.I-vials yeglasiNgokukodwa zihlala zinokuphinda zisetyenziselwe ukuphinda zikhethwe, kodwa zibalulekile ukuze ujonge kwiindawo zokurisayikilisha zendawo ngeemfuno zabo ezithile. Ii-Vials kufuneka zitshiswe ngokupheleleyo ngaphambi kokuba ziphinde zisebenze kwakhona ukuze zihlangabezane nemigangatho yokucoceka.

Inyathelo 5.Imigaqo yokulahla inkunkuma

Ukuthotyelwa kweMigaqo yeNdawo, kwiLizwe, kunye neMibuliso yokulawula ukuchithwa kwenkunkuma yelebhu yiparamount. Ezinye izikhokelo zinezikhokelo ezithile zokuchithwa kwenkunkuma enobungozi, kwaye iilebhu kufuneka zihlale zangoku ukuthintela iziphumo ezisemthethweni. Ukuthotyelwa kwale migaqo kuya kukhuthaza inkqubo ekhuselekileyo neyisingqongileyo.

Inyathelo 6.Nxibelelana nolawulo lwenkunkuma

Ukuba ukungaqiniseki kuphatha ngeendlela zokulahla izinto okanye ii-vials zinezixhobo eziyingozi, kuyacetyiswa ukuba iinkqubo zolawulo lwenkunkuma ziboniswe. Ezi bhuti zinokubonelela ngesikhokelo esibalulekileyo kwiindlela ezikhethekileyo zokulahla inkunkuma kwaye ziqinisekise ukuba ilabhoratri yakho ihambelana nemigaqo yokhuseleko.

Inyathelo 7.Ukutshiswa

Kwezinye iimeko, ukutsalwa kunokuba yindlela efanelekileyo yokulahla inkunkuma ye-vials, ngakumbi ezo zingcolise izinto eziyingozi. Nangona kunjalo, kubalulekile ukuba ulandele iinkqubo ezimiselweyo kwaye ufune isikhokelo kubasebenzi boLawulo lweNkunkuma ukuqinisekisa ukuba ukutshiswa ngokukhuselekileyo kunye nokuthintela iimpembelelo ezichaseneyo zendalo.

Ukulahlwa kwendawo efanelekileyoI-chialsgraphy ye-chromatographyAyisiyomfuneko nje kuphela, kodwa noxanduva lokuba iilebhu zinezilaratri zoKhuseleko nolwesikelelekeni. Ngokulandela iinkqubo ezichaziweyo kunye nokuhlala unolwazi ngemigaqo yendawo, iilabhoratri zinokuba negalelo kwiindlela zokulawula inkunkuma ezizinzileyo. Ukugcina imigangatho yolawulo lwelebhu enoxanduva, ihlala ibeka phambili ukhuseleko kunye nokuthotyelwa xa uphatha kunye nokulahla i-vialsgraphy ye-chials.

Ndinomdla malunga ne-vials ye-HPLC? Jonga iimpendulo ezingama-50 kweli nqaku. Yijonge ngoku !:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

I-chiromatography i-vials yesampulu yendalo kunye nohlalutyo

Okulandelayo:

Kutheni i-ptfe \ / isiliki septa esetyenziswe kwi-chromatography? Izizathu ezi-5