Aug. 30th, 2024
Intsebenzo ephezulu ye-chromatography (i-HPPC) yindlela ephambili yohlalutyo esetyenziselwa iilebhu ezikwimimandla njengamayeza, iisayensi zendalo esingqongileyo, kunye nokhuseleko lokutya. Kwilizwe le-HPLC, kukho iindidi ezimbini eziphambili: Uhlalutyo lwe-HLLC. Ngelixa zombini iindlela zobugcisa zisebenzisa imigaqo efanayo yokwahlulahlulahlulo, zisetyenziselwa iinjongo ezahlukeneyo kwaye zineeparamitha ezahlukeneyo zokusebenza. Le bhlog iya kuphonononga umahluko phakathi kohlalutyo lwe-HLLC, kubandakanya injongo, ubungakanani, izixhobo, kunye nokusetyenziswa.
Ufuna ukwazi ngokupheleleyo malunga ne-vials ye-HPLC, nceda ujonge eli nqaku: I-Encyclopedia ye-vials ye-HPLC
Ukuqonda i-HPPC yohlalutyo
Inkcazo kunye nenjongo
I-HPPC yohlalutyo igxile ikakhulu ekuphumezeni nasekuchongeni imigaqo kwisampulu. Eyona njongo iphambili kukufumana ulwazi olufanelekileyo kunye nolwazi malunga nezinto ezikhoyo kumxube. I-HPPC yohlalutyo ihlala isetyenziselwa:
Uhlalutyo olufanelekileyo: Ukuchonga izinto zesampulu.
Uhlalutyo lobungakanani: Ukumisela ukugxininiswa kwenxalenye ethile kwisampulu.
Ubungakanani bokusebenza
I-HPLC yohlalutyo isebenza kumgangatho omncinci, ebandakanya inaliti ye-microroriater. Iivolumu ezisetyenzisiweyo ngokubanzi zikwi-1 ukuya kwi-100 μl yoluhlu, zivumela iisampulu ezininzi ukuba zihlalutyelukiswe kwisithuba esinye. Eli zithuba lincinci lilulungele ukuvavanywa kwesiqhelo kunye nolawulo oluphambili.
Ubungakanani bekholamu kunye ne-paricle ubungakanani
Iikholamu ezisetyenziswa kwi-HPPC yohlalutyo zihlala zincinci ubukhulu, zihlala zijikeleze i-4.6 mm, kwaye zipakishwe ngamasuntswana amancinci amancinci (3-5 μm). Olu hlengahlengiso luvumela ukusonjululwa okuphezulu kunye nokwahlukana okusebenzayo, kufanelekile uhlalutyo oluneenkcukacha.
Iindlela zokufumanisa
I-HPPC yohlalutyo isebenzisa iindlela zokuchonga, kubandakanya i-UV-ebonakalayo, ifafu, kunye ne-spectrometry. Ukukhetha komnxibelelanisi kuxhomekeke kubunjani bohlalutyo kunye neemfuno ezithile zohlalutyo. Oovimba abangonakalisiyo bahlala besetyenziswa ukuqinisekisa ukuba isampulu inokuhlaziywa ngaphandle kwelahleko enkulu.
Ufuna ukwazi iimpendulo ezingama-50 malunga nee-vials vials, nceda ujonge eli nqaku: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL
Ukuqonda i-HPLC ye-HPPL
Inkcazo kunye nenjongo
Ukulungiselela i-HPLC, kwelinye icala, ijolise ekwahlukanisweni kwaye ihlambulule imixube ethile kumxube. Eyona njongo iphambili kukukhetha ubungakanani obaneleyo bethagethi ekujoliswe kuyo yohlalutyo okanye ukusetyenziswa. Ilungiselelo
I-HPPC isetyenziselwa ezi zicelo zilandelayo:
Ukuziqhelanisa nokuzibandakanya
Ukulungiswa kwesampulu: Ukulungiswa kweesampulu zohlalutyo olulandelayo okanye ukwenziwa kwenkqubo.
Ubungakanani bemisebenzi
I-HPPC yokulungiselela yenziwa kwinqanaba elikhulu kune-HLPC yohlalutyo. Iisampulu zesampulu zinokuvela ukusuka kwi-milliliters ezimbalwa kwiilitha ezininzi, kuxhomekeka kwisivuno esilindelekileyo secandelo ekujolise kulo. Lo mgangatho mkhulu uvumela inani elaneleyo lezinto ezihlanjululweyo ezinokuqokelelwa ukuze zisetyenziswe.
Ubungakanani bekholamu kunye ne-paricle ubungakanani
Iikholamu ezisetyenziselwa ukulungisa i-HPLC inkulu kakhulu, iqhele ukusukela kwi-20 mm ukuya kwi-100 mm okanye enkulu kububanzi. Iikholamu zipakishwe ngamasuntswana amanqanaba amakhulu (Ngokwesiqhelo i-10 ukuya kwi-20 μm) ukukhulisa amaxabiso okuhamba kunye nokufumana imiqulu emikhulu yesampulu. Olu luyilo lunceda ukunciphisa uxinzelelo lwangasemva kwaye kufezekise ukwahlulwa okufanelekileyo kwemigaqo.
Iindlela zokufumanisa
Kwindlela yokulungiselela i-HLLC, iindlela zokuchonga zinokwahluka kwezi zisetyenziswa kwi-HPPC yohlalutyo. Ngelixa izixhobo ezingonakalisiyo zisaxhaphakile, iinkqubo zokulungiselela zihlala zisebenzisa izixhobo ezinokwazi ukuqokelela amaqhezu asekwe kwiprofayili ye-Elpres. Oku kuvumela amakhonkco othile ukuba abekwa ngaphandle njengoko besizi kwikholamu.
Ufuna ukwazi ngakumbi malunga nokusetyenziswa kwe-vialsgraphys, nceda ujonge eli nqaku:Iindlela ezintlanu zokucoca ii-vials ze-HPLC
Umahluko ophambili phakathi kokuhlalutya kunye nokulungisa i-HPLC
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Inqaku
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I-hplc yohlalutyo
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I-hplc yokulungisa
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Injongo
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Uhlalutyo lwenqanaba kunye nobungakanani
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Ukuhlanjwa kunye nokuhlanjululwa kweekhomputer
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Ubungakanani bokusebenza
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Amanqanaba amancinci (μl's)
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Isikali (ml kwi-LA GLOOS)
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Ubungakanani bekholamu
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Ububanzi obuncinci (i-4.6 mm)
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Ububanzi beNqaku (i-20-100 mm)
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Ubungakanani bentsundu
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Amasuntswana amancinci (i-3-5 μm)
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Amasuntswana amakhulu (10-20 μm)
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Ukuhamba kweereyithi
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Ireyithi yokuhamba ephantsi (0.1-5 ML \ / min)
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Irhafu ephezulu yokuhamba (i-10-200 ml \ / min)
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Iindlela zokufumanisa
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Abachongi abangakumbi (i-UV, ms)
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Ukuqokelelwa kwenkolelo esekwe kwiprofayili ye-Elpress
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Ukukhetha phakathi phakathi kohlalutyo kunye nokulungisa i-HPPCC
Xa ukhetha phakathi kokukhetha uhlalutyo kunye nokulungiselela i-HPLC, cinga ngezi zinto zilandelayo:
Injongo yohlalutyo: Chonteza ukuba ngaba eyona njongo iphambili kukungafundiswanga kwaye kuhlalutye ngamanzi okanye ukwahlula amakhonkco okuthengiswayo ukuze asebenzise kwakhona.
Isixa sesampulu: Vavanya isixa sesampulu esikhoyo. Ukuba isixa esincinci sesampulu sifumaneka, i-HPPC yohlalutyo inokuthi ifaneleke ngakumbi. Kwelinye icala, ukuba isixa esikhulu sesampulu siyadingeka, i-HPLC yokulungiselela kukhetho olungcono.
Ndifuna ukucoceka kunye nesivuno: Cinga ngococeko olufunekayo kunye nesivuno secandelo ekujolise kulo. Preparative HPLC aims to maximize the recovery of a specific compound, while analytical HPLC focuses on providing detailed information about the composition of the sample.
Izixhobo kunye nezixhobo: Vavanya izixhobo ezikhoyo, kubandakanya izixhobo kunye nohlahlo-lwabiwo mali. Iinkqubo zokulungiselela i-HLLC zihlala zibiza kakhulu kwaye zifuna utyalomali ngakumbi kwizixhobo nakwiindleko zokusebenza.
Ndinomdla kwi-HLLC ye-HPLC ye-HLLC? Jonga eli nqaku:I-HLLC yesampulu yesampulu yeziphumo zeziphumo ezilungileyo.
Ukuqukumbela
Zombini i-HPLC yohlalutyo kunye nokulungisa i-HPLC zibaluleke kakhulu kwiindlela ze-chromatography, kwaye nganye inezinto ezahlukeneyo ngokuxhomekeka kwiimfuno zabaphandi kunye nomzi mveliso. Ngelixa i-HPPC yohlalutyo ilungele uhlalutyo oluneenkcukacha kunye nokudityaniswa kweekhompawundi, i-HPPC yokulungiselela ibalulekile ekwenzeni nasekuthinteleni amakhonkco okuthintela ezinye izicelo. Ukuqonda umahluko phakathi kwezi ndlela zimbini kuya kunceda abaphandi benze izigqibo ezizizo, bafundise indlela yokusebenza kwabo, kwaye bafumane iziphumo ezichanekileyo nezichaphazela iziphumo ezichanekileyo nezinokuthenjwa kuhlalutyo lwabo. Njengoko itekhnoloji ye-HLPC iyaqhubeka ukuqhubela phambili, zombini iindlela zokuhlalutya kunye nokulungisa ziya kuhlala ziyinxalenye yophando oluyimpumelelo lwezenzululwazi lwazo zonke iintlobo.
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