I-Spoas Spirical Sport Gossions ye-GC-NS

I-chromatography-mass spectrometry (GC-MS) yindlela enamandla yohlalutyo esetyenziselwa ukuhlalutya kunye ne-semivolatile. Kuxhomekeka kubume besampulu kunye nohlali ekujoliswe kuzo, iindlela ezahlukeneyo zobuchwephesha zinokuqeshwa ngokufanelekileyo ukulungisa isampulu. Oku kulandelayo ziindlela eziqhelekileyo ezisetyenziselwa ukulungiselela iisampulu zeUhlalutyo lwe-GC-DS:

Ufuna ukwazi ngakumbi malunga nomahluko phakathi kwe-LC-Nks kunye ne-GC-Nks, nceda ujonge eli nqaku:Nguwuphi umahluko phakathi kwe-LC-Nks kunye ne-GC-Nks?

I-1. Ukulungiselela isampulu yesampulu

I-dilution: Iisampulu ezingamanzi zihlala zithathwe kwindawo yokubila ephantsi enjengee-methanol, i-acetone, okanye i-dichtone, okanye i-dichloromethane yokufezekisa i-0,1 mg \ / ml. Oku kuqinisekisa ukuba isampulu iyahambelana nenkqubo ye-GC kwaye inciphise umngcipheko wokuvala i-inlet.

Ukuqulunqa AI-0.22 μm icebo lokucocaihlala isetyenziswa.

I-Tentrifordation: Kwisampuli enokuthi ibe ne-Soltidid, i-khulurrigation inceda ukwahlula ulwelo lube nakuphi na izinto ezingafunekiyo ngaphambi kokudluliselwa kwi-vial.

I-2. Ukulungiswa kwesampulu

Ukuchithwa: Iisampulu eziqinileyo kufuneka zichithwe kwinqaku elifanelekileyo lokulimala. Yongeza isixa esincinci (iingqolowa ezimbalwa) esiqinileyo kwi-vial ye-solvent kwaye iguqulele izihlandlo ezininzi ukuqinisekisa ukutshitshiswa ngokupheleleyo.

I-Derivatisisetion: ye-Semi-ratile okanye i-polar quands, ukugxothwa kunokuba yimfuneko ukonyusa ingqibelelo kunye nokuphucula iimvakalelo zokuchonga. Oku kubandakanya ukuguqula ngokufanelekileyo imichiza ukuyenza ibe nzima ngakumbi kuhlalutyo lwe-GC.

3. Uhlalutyo lwentloko

I-Static Stace: Kule ndlela, i-vial evaliweyo enesampulu ibanjelwe kwiqondo lokuhlala liqinisekile ukuvumela amabala okuxineneyo ukuze afumane isampulu ngaphezulu kwesampulu. Nje ukuba ulingane kufikelelwe, le thuba le-Sepcan linokupasa kwi-hlalutyo usebenzisa isirinji yegesi.

I-Synamic Propace (ukwehla kunye nomgibe): Obu buchule lubandakanya ukudlulisa irhasi ye-InErt ngesampulu yokuphucula amandla okukhutshwa kweplate. Le ndlela inyusa kakhulu ubuntununtunu ngokugxila kwi-windows windows engaphambi kohlalutyo.

Ngaba ufuna ukwazi ngakumbi malunga nokuba kutheni i-villa ye-Promatoces esetyenziswe kwiChromatography ?, Nceda ujonge le arcece: Kutheni le nto i-villa ye-ERPE isetyenziswa kwiChromatography? Ii-Angles ezili-12

4. Iindlela zokukhupha

Isigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba (SPME): I-SPME isebenzisa i-fiber edityaniswe nesigaba sokukhupha ukuze ifumane uhlalutyo ukusuka kwinqanaba lolwelo okanye igesi. Obu buchule buvumela isampulu ngqo ngaphandle kwesidingo sokuzithoba kwaye iluncedo kakhulu kwiikona eziguquguqukayo.

Ukukhupha i-Liqhele

5. Iingcebiso zokugxila

Ukuhlasela i-nitrogen

Ukuqwalaselwa kweSampulfowuni

Qinisekisa ukuba zonke izinyithi zisetyenzisiwe ziguqukile kwaye zilungele i-GC-Nk; Amanzi kunye nezifo ezingafakwanga kufuneka zithintelwe.

Iisampulu akufuneki ziqulathe naziphi na ii-asidi ezizomeleleyo, iziseko, iityuwa, okanye ezinye izinto ezingcolisekileyo ezinokonakalisa ikholamu ye-GC okanye iphazamisa uhlalutyo.

Iisampulu zokugqibela kufuneka zikhululwe ngamasuntswana kwaye zikhethwe ngokukhethekileyo ngaphakathii-vials yeglasi Ukuthintela ukwanda kwezinto ezivela kwiplastiki.

Ngolwazi oluthe kratya malunga ne-vials ye-autosampler ye-chromatography, ibhekisa kweli nqaku: I-2 ml automampler vials ye-chromatography yegesi

Ukuqukumbela

Iindlela ezifanelekileyo zokulungiselela iisampulu zibalulekile kuhlalutyo lwe-GC-ms ye-GC-ms. Indlela nganye inezibonelelo zayo kwaye inezicelo ezithile ngokuxhomekeke kubunjani besampulu kunye nethagethi ekujoliswe kuyo. Ngokuqesha ezi ndlela, abahlaziyi banokuphucula ukuchaneka, ubuntununtunu, kunye nokuzala kweziphumo zabo, ekugqibeleni bafumana idatha ethembekileyo kwimimandla eyahlukeneyo, ukhuseleko lokutya, kunye namayeza.