I-chromatography-mass spectrometry (GC-MS) kunye ne-chromatography ye-chromatography (HPLC) ziindlela ezimbini eziphambili ezisetyenziselwa ukwahlula, kunye nokudibanisa iindidi zeesampulu ezahlukeneyo. Indlela nganye inezibonelelo zayo ezizodwa kwaye ilungele iintlobo ezahlukeneyo zohlalutyo. Ukuqonda umahluko osisiseko phakathi kwe-GC-Nks kunye ne-HPPC kubalulekile ekukhetheni ubuchwephesha obufanelekileyo ngokusekwe kubume besampulu kunye neemfuno ezithile zohlalutyo.

Ufuna ukwazi iimpendulo ezingama-50 malunga nee-vials vials, nceda ujonge eli nqaku:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Umahluko phakathi kwe-GC-NS kunye ne-hppc

1. Inqanaba le-Mobile

Umahluko ophambili phakathi kwe-GC-Nks kunye ne-HPPC lisigaba sefowuni. I-GC -S isebenzisa isigaba se-Gaseous, ihlala irhasi ye-intert enje nge-helium okanye initrogen, ukuhambisa isampulu ekhutshiweyo ngekholam yeChromatographic. Oku kwenza ukuba i-GC-ms ilungele ngokukhethekileyo ekuhlalutyeni imizobo eguqukayo ngokulula kumaqondo obushushu aphezulu.

Ngokwahlukileyo, i-HPPC isebenzisa iSigaba seSigaba seLizwe, idla ngokuba ngumxube we-Solvent esekwe kwi-polaty kunye nokususwa kwesampulu. Oku kwenza ukuba i-HPLC yokuhlalutya uluhlu olubanzi lwezinto ezihlanganisiweyo, kubandakanya zombini izinto eziguqukayo nezingaphindiyo.

2. Uhlobo lwesampulu

Iindidi zeesampulu ezinokuhlalutywa yindlela nganye ziyahluka kakhulu. I-GC-Nks ilungele ukuhlalutya amabala okuhlalutya okanye eziguquguqukayo, ezifana ne-hydrocarbons, ioyile eyimfuneko, kunye nokungcoliseka kwendalo. Ayisebenzi kangako ukuba ubushushu obushushu okanye ubushushu. I-HPLC, kwelinye icala, inokuphatha uluhlu lweesampulu, kubandakanya imibandela yephalari, ii-biolecules, amayeza, kunye nomxube onobunzima obunokuqulatha ii-SALTS okanye iindidi ezizezinye. Okuhlumazekileyo kwenza i-hppc inketho ephezulu kwimimandla efana ne-biochemists kunye namayeza.

I-Wan Ukufumana ulwazi olupheleleyo malunga nendlela yokucoca i-vialsgraphy yesampulu ye-chromatography, nceda ujonge eli nqaku:Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys

3. Iimeko zobushushu

Iqondo lobushushu lidlala indima ephambili kwiindlela zombini, kodwa ngeendlela ezahlukeneyo. I-GC-MS isebenza kumaqondo obushushu aphezulu, ngokuqhelekileyo phakathi kwe-150 ° C kunye ne-300 ° C, ukuqinisekisa ukuphuma okusebenzayo kwesampulu. Eli nqanaba lobushushu i-right ivumela uhlalutyo olukhawulezileyo, kodwa lubeke umda kwiisampulu ezinokuthi zihlalutywe, njengokuqulunqa ubushushu. Ngokwahlukileyo, i-HPPC idla ngokwenziwa kumaqondo obushushu okanye amaqondo obushushu aphezulu, esenza ilungele ukuhlalutya imida ye-Ubushushu ngaphandle komngcipheko wokuphosa.

4. Indlela yokwahlula

I-GC-Nks kunye ne-HPPC ineendlela ezahlukeneyo zokwahlula-hlula ngenxa yezigaba ezahlukeneyo zesoftware. Kwi-GC-Nks, ukwahlukana kusekwe ikakhulu kukuzinza kwekhomputha; Iicomputer ezingalunganga zinxibelelana ngakumbi nenqanaba lesikhululo kunye ne-elute ngakumbi kancinci kunemigangatho engaphezulu.

Ngokwahlukileyo, i-HPPC yahlulahlula amakhonkco ngokusekwe kunxibelelwano lwabo kunye nezigaba zeselula kunye nezikhululo, ezigqitywe zizinto ezinje ngokuzenzekelayo. Imimandla ye-polar iqhele ukuhamba ngekholamu ngokukhawuleza kuba zinomdla kakhulu kwisigaba sefowuni.

I-5. Iindlela zokufumanisa

Iindlela zokufunyanwa eziqashwe ngu-GC-Nks kunye ne-HPPC zahluke kakhulu. I-GC-mis idibanisa i-chromatography yegesi kunye ne-spectrametry, evumela ukufumanisa ubutshintsho ngokusekwe kumlinganiso wabo ohlawula ubunzima becala emva kokwahlukana. Oku kudityaniswa kubonelela ngolwazi oluneenkcukacha ngesakhiwo malunga nohlalutyo. Okuchasene,HlcNgokwesiqhelo isebenzisa i-spectratometry ye-UV-ebonakalayo ye-Index Ngelixa ezi ndlela ziyasebenza kwizicelo ezininzi, zinokubonelela ngolwazi oluncinci lwesakhiwo kunombala we-spectrometry.

I-6. Izixhobo kunye nokuqwalaselwa kweendleko

Izixhobo eziyimfuneko kwi-GC-Nks kunye ne-HPPC yahluka kakhulu ngokuhambelana nobunzima kunye neendleko. Iinkqubo ze-GC ngokubanzi zilula kakhulu; Bafuna ukubonelela ngegesi (igesi yomphayi) kodwa hayi impompo yoxinzelelo oluphezulu kuba iigesi zinophawu olusezantsi kunetywina. Oku kuko ngokubanzi kwenza iinkqubo ze-GC zingabizi kakhulu ukusebenza kwixesha elide. Ngokwahlukileyo, iinkqubo ze-HPLC zifuna impompo yoxinzelelo oluphezulu ukutyhala i-Solven yoLwandle ngokugcwaliswa kwekholam ezaliswe sisigaba sesikhululo, kwaye inzima kakhulu ukugcina ngenxa yemfuno yezinyithi ezikhethekileyo.

Ukukhetha phakathi kwe-GC-NS kunye ne-HPPC

Xa usenza isigqibo sokuba usebenzise i-GC-Nks okanye i-HPPC, kukho izinto ezininzi ekufuneka uziqwalasele:

Ubume besampulu yakho: Chonge ukuba isampulu yakho iguqukile okanye ingenzi.

Uzinzo lwe-therm: Vavanya ukuba ngaba uhlalutyi bakho ungakwazi ukumelana namaqondo obushushu aphezulu ngaphandle kokuthambisa.

Uvelwano olufunekayo: Qwalasela ukuba ufuna ulwazi oluneenkcukacha zesakhiwo (oluthando gc-ms) okanye nje imilinganiselo yoxinaniso (enokwenziwa nge-HPPC).

Izithintelo zeendleko: Vavanya uhlahlo-lwabiwo mali lwakho lokuthenga izixhobo zokuthenga kunye nokugcinwa.

Kwisishwankathelo, zombini i-GC-Nks kunye ne-HPPC zibaluleke kakhulu kwizixhobo zohlalutyo, kwaye indlela nganye inezibonelelo zezicelo ezithile. Ngokuqonda umahluko wabo osisiseko (E.G., isigaba sefowuni, indlela yesampulu, indlela yokuqalisa, indlela yokuqaphela, kunye neendlela zokuqaphela, kunye nezazinzulu zinokwenza ngcono ukuba yeyiphi itekhnoloji yeTekhnoloji.

Ufuna ukwazi ngakumbi malunga nomahluko phakathi kwe-LC-Nks kunye ne-GC-Nks, nceda ujonge eli nqaku:Nguwuphi umahluko phakathi kwe-LC-Nks kunye ne-GC-Nks?

Ngaphambili:

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Okulandelayo:

I-Spoas Spirical Sport Gossions ye-GC-NS

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