Ithini ifuthe lobungakanani be-vial kwiziphumo zeChromatography?

I-chromatography yindlela esetyenziswa kakhulu kwi-chemistry yecachestral, i-biochemistry, kunye neendidi zemimandla eyahlukileyo kwaye zihlalukene nezinto kumxube. Inkalo ebaluleke kakhulu yeChromatography ihlala ijongwa bungakanani ubukhulu be-vial esetyenziselwa ukubamba isampulu. Ukukhetha ubungakanani bobuqhetseba kunokwenza ifuthe kakhulu ukuchaneka, ukuzala kwakhona, kunye nomgangatho opheleleyo weziphumo ze-chromatographic. Eli nqaku lifungela imiphumo eyahlukeneyo yobungakanani be-vial kwi-chromatography, egxile kwiinguqulelo zesampulu, iziphumo zentloko, umngcipheko wokungcola, kunye nokuhambelana neendlela zokuhlalutya.

Ukuqwalaselwa kweqanda

Umthamo wesampulu yesampulu yenye yezona zinto ziphambili ekuthandeni ukukhetha ubungakanani be-vial. I-vialsgraphy yeChromatography iyafumaneka kwiisayizi ezahlukeneyo, idla ngokusukela kwii-vials ezincinci (0.3 ml) kwi-SITER eziqhelekileyo (i-1.5 ml kunye ne-2 ml).

I-vials ezincinci: Ezi zilungele imiqulu emincinci yesampulu kwaye ziqhele ukusetyenziswa kwizicelo eziphezulu zobuntu ezingenamsebenzi we-chromatography (i-HPPC) kunye ne-chromatography (GC). Ubungakanani babo abancinci buncitshise inkunkuma kwaye ivumela uhlalutyo oluchanekileyo xa isampulu inomda.

Ii-vials eziqhelekileyo: I-Vials ezinkulu ihlakulela imitha enkulu kodwa inokukhokelela kwimiba ukuba ayihambelani kakuhle kwisayizi yesampulu. Ukugqithisa iintloko kunokubangela ukuvela okanye ukonakaliswa kweendlela eziguqukayo, ezikhokelela kwiziphumo ezichanekileyo.

Ukukhetha i-vial ehambelana ngokusondeleyo ivolumu yesandla efunekayo iqinisekisa ukuba inkqubo yeChromatography isebenza ngokufanelekileyo ngelixa igcina ingqibelelo yesampulu.

Sifuna ukwazi ukuba kutheni i-villatography yeGramatography ilunge ngakumbi kune-vials yeplastiki, nceda ujonge eli nqaku: Izizathu ezi-3 eziphezulu zokuba kutheni iGlasi chromatography ye-vials ilunge ngakumbi kune-vials yeplastiki

Iziphumo zemigaqo

IPasika ibhekisa kwisithuba ngaphakathi kwe-vial ngaphezulu kwesampulu yolwelo. Indawo epheleleyo yentloko ibalulekile ekugcineni iimeko ezingaguqukiyo ngexesha lohlalutyo.

Ingozi yokuphuma: Ukuba i-vial inomgangatho omkhulu kakhulu kwivolumu yesampulu, amacandelo okuguqula inokuphuma, ukutshintsha amanqanaba oxinzelelo kunye neziphumo ezinokulalanisa. Oku kubaluleke ngakumbi kwizicelo ze-GC apho iziqu eziguqukayo ziyahlalutya.

Ukungqinelana kwenaliti: I-Inaction yamaje ayinakuphuma kulawulo olungafanelekanga lweentloko. Ukuqinisekisa ukuba ubungakanani be-Vial ilungele umthamo wesampula kunceda ukugcina imeko yezinzile ngaphakathi kwe-vial, kukhokelela kwiinaliti ezivelisiweyo.

Umngcipheko wokungcola

Umbandela kunye nobungakanani beI-chialsgraphy ye-chromatographyikwaphembelela umngcipheko wokungcola. Izixhobo ezahlukeneyo ezibonisa amanqanaba ahlukeneyo okunxibelelana neesampulu.

Ukukhethwa kwezinto ezibonakalayo: Ii-vials zeglasi zikhethwa ngokubanzi ngenxa yepropathi yazo, enciphisa umngcipheko ongcolisekileyo ukusuka kwizinto ezivunyiweyo. Nangona kunjalo, i-vials zeplastiki inokulungela izicelo ezithile, ezinjengokuvavanywa kwe-PFAS. Ukhetho lwezinto ezibonakalayo kufuneka luhambelane nobume bemichiza yohlalutyiweyo.

Unxibelelwano lomphezulu: Iiproteni kunye ne-acid ye-nucleic zinokwenza kwindawo yeglasi, ekhokelela ekunciphiseni amaxabiso okubuyisela kunye nendlela yokubuyisela. Umzekelo, iiproteni ze-hydrophobic zinokwenza ityiwa i-dududus ngeglasi ngokuhamba kwexesha, ukuhlalutya okunzima. Ukunciphisa le micimbi, i-vials ekhethekileyo ye-adsorves okanye i-vialproplene ye-polyproplene eyenzelwe ii-bimolecules ezibuthathaka ziyacetyiswa.

Ukuhambelana neendlela zohlalutyo

Iindlela ezahlukeneyo ze-chromatografi zinokufuna ubungakanani be-vial kunye neentlobo zokuqinisekisa ukusebenza kakuhle.

Iimfuno ze-HLLC kunye ne-GC: Izitayile zokuvalwa kwe-vial (i-screw caps vs) inokuchaphazela kakhulu ukuthembeka ngexesha lohlalutyo ngexesha lohlalutyo.Crimp capszihlala zisetyenziswa kwi-vials ye-GC ukuya kwi-Seal ekhuselekileyo, ngelixaI-CAPS CAPSIngafaneleka ngakumbi kwizicelo ze-HLLC apho kunokwenzeka ukufikelela khona ukufikelela.