Kutheni i-HPPC ibalulekile kuhlalutyo lokusingqongileyo

Intsebenzo ephezulu ye-chromatography (i-HPPC) yindlela ebalulekileyo yohlalutyo esetyenziswa ngokubanzi kuhlalutyo lokusingqongileyo ukufumana, ukulungisa i-poltles, umhlaba kunye nomoya. Njengoko inkxalabo malunga nokuthathwa kwendalo okubangelwe yimisebenzi yemizi-mveliso, ukufudukela kwabantu ezidolophini, kunye neendlela zezolimo, i-HPCC iba sisiseko sokuqinisekisa ukhuseleko lwendalo kunye nokuthotyelwa kwemigangatho yolawulo.

I-HPLC yindlela entsonkothileyo eyahlulahlula izinto kumxube ngokusekwe kunxibelelwano lwabo nezigaba zesitishi I-HPLC ifumana isisombululo esiphezulu kunye nemvakalelo ngokufaka uxinzelelo ukunyanzela isampulu yetroli ngekholam ezaliswe zizixhobo zesikhululo samanyathelo. Eli nqaku linokufumana umkhondo wokungcolisa okungathandekiyo kwiindlela ezincinci ezibuthathaka.

Ufuna ukwazi ngakumbi malunga nokulungiselela isampulu ye-HPLC, nceda ujonge eli nqaku:I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo

Ukusetyenziswa okuphambili kwe-HPPC kuhlalutyo lwendalo

1. Uvavanyo lweMgangathweni wamanzi

Esona sixhobo siphambili se-HPLC kuhlalutyo lokusingqongileyo kukuvavanywa komgangatho wamanzi. Izizathu ezinjengezinyithi ezinzima, izibulala-zinambuzane, amayeza, kunye ne-endocrine yokuphazamisa iichemicals zinokubeka emngciphekweni omkhulu kwimpilo yabantu kunye ne-Equsystem yendalo. I-HPPC ikuvumela ukuba:

Ukuchonga okungcolileyo: I-HPPC inokuchonga kwaye ichaze izinto ezinobungozi kwindawo ephantsi kakhulu kwiisampulu zamanzi. Umzekelo, izinambuzane ezihlala zifumaneka kwizolimo zinokufunyanwa.

Ukubek'esweni ukuthotyelwa: Iiarhente ezilawulayo zifuna ukubeka esweni rhoqo amaziko amanzi ukuqinisekisa ukuthotyelwa kwemigangatho yokhuseleko. I-HPPC ibonelela ngedatha ethembekileyo enceda abasemagunyeni bathabatha amanyathelo afanelekileyo ukukhusela impilo yoluntu.

2. Uhlalutyo longcoliseko lomhlaba

Umhlaba yenye iMatrix ebalulekileyo yokuBeka iliso kwindalo esingqongileyo kuba inokuqokelela ii-poltleints eziveliswe yimisebenzi yezolimo, imisebenzi yemizi mveliso, kunye nokulahlwa kwenkunkuma. I-HPLC idlala indima ebalulekileyo koku kulandelayo:

Ukuchongwa kwe-Pillballungs: i-HPPC kuyanceda ukuvavanya ubungakanani bongcoliseko ngokuhlalutya izinto ezifana ne-polycyclic hydrocarbons (i-pahs), kunye nezibulala-zinambuzane kwiisampulu zomhlaba.

Amanyathelo okuhlaliswa kovavanyo: Kwiinkqubo zebhayimu ezijolise ekucoceni iindawo ezingcolisekileyo, i-HPCC inokuvavanya ukusebenza kweendlela zonyango ngokunyanzeliswa kwexesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcolisa ixesha lokungcola ngokuhamba kwexesha.

I-3. Ukubeka iliso kumgangatho womoya

I-HPLC ikwasetyenziswa ukuhlalutya ukungcola komoya kunye nokubeka iliso kumgangatho womoya. Izicelo zibandakanya:

Uvavanyo lwe-Orlatile (VOC): VOCs ziikhemikhali eziyingozi ezikhutshwe kwimithombo eyahlukeneyo, kubandakanya iipeyinti, izinyithisi, kunye neenkqubo zokumiswa. I-HPPC inokwahlulahlula kwaye icombulule ezi zikhombisi kwiisampulu zomoya.

Uhlalutyo lwentsumpa: Obu buchule bunceda ukuchonga amasuntswana anobungozi anokuchaphazela impilo yokuphefumla kunye negalelo kungcoliseko lomoya.

4. Uhlalutyo lweNkunkuma

Uphawu lwenkunkuma lubalulekile ekulahlweni okufanelekileyo kunye nokurisayikilisha. I-HPPC inokukunceda:

Hlalutya imijelo yenkunkuma: I-HPPC inceda ekumiseleni indlela efanelekileyo yokulahla inkunkuma ngokuchonga izinto eziyingozi zenkunkuma.

Ukuqinisekiswa kokuchithwa okukhuselekileyo: Ukuthotyelwa kolawulo kufuna ukuba inkunkuma ihlalutywe ngokupheleleyo ukunciphisa umngcipheko wokusingqongileyo onxulumene nokulaliswa okungafanelekanga.

Ufuna ukwazi ngakumbi malunga nokusetyenziswa kwe-vialsgraphy ye-vials, nceda ujonge eli nqaku: I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

IINGXAKI ZOKUSETYENZISELWA I-HLPC yohlalutyo lwendalo

Kukho izibonelelo ezininzi ezibalulekileyo ukusebenzisa i-HPPC kuhlalutyo lokusingqongileyo.

I-1. Ubuntu obuphezulu kunye nokuchaneka

I-HPLC inokufumanisa ukuba i-Platices Amanqanaba engcoliseko, esenza sisixhobo esinamandla sokubeka iliso kungcoliseko olunokuba nefuthe lendalo okanye lempilo. Oku kubuntununtunu obuphezulu kuvumela ukufunyanwa kwangoko kweengozi ezinokubakho ngaphambi kokuba zikhule zibe ziingxaki ezinkulu.

I-2. UQhakamshelwano

I-HPLC ibe sisixhobo esiguquguqukayo sezazinzulu zenzululwazi ngendalo kuba inokuthi ihlalise iintlobo zohlalutyo kumatriki eyahlukeneyo (amanzi, umhlaba, umoya, inkunkuma, inkunkuma, inkunkuma. Iindidi ezahlukeneyo zamachaphaza (i-UV-Vis, ifluorescence, i-spectrometry, njl. Njl.) Inokusetyenziswa ngokuxhomekeka kwiimfuno ezithile zohlalutyo.

3. Ukomelela

Ubungqingqwa beenkqubo ze-HPLC zenza ukuba bakwazi ukuphatha imatriki abaxineneyo efumaneka kwiisampulu zokusingqongileyo. Iindlela ezifanelekileyo zokulungiselela amalungiselelo, ezinjengokusungulwa kwesigaba esiqinileyo (Spe) kunye nokufakelwa, kunokonceda abahlalutyi bezinto ezichaphazela iziphumo.

Ufuna ukwazi ngakumbi malunga ne-vialsgraphy ye-vials yohlalutyo lwamanzi, nceda ujonge eli nqaku: I-chillsgraphy ye-chromatography yohlalutyo lomgangatho wamanzi

Ukusebenza nzima kwe-chromatography ephezulu kakhulu kumi njengesixhobo sohlalutyo esibonakalayo kuhlalutyo lokusingqongileyo ngenxa yobuntununga bakhe obungafaniyo, nokungabi gwenxa, kunye nokomelela. Izicelo zayo zisiphelo kuvavanyo lomgangatho wamanzi, uhlalutyo longcoliseko lomhlaba, ukubekwa kweliso lomgangatho womoya, kunye neNkunkuma yeNkunkuma - nganye nganye ifaka igalelo ekuqondeni kwethu iingxaki sempilo yokusingqongileyo. Njengoko ulwazi lwehlabathi malunga nongcoliseko lunyuka ngeemfuno ezikwimeko yokucoca indawo, indima ye-HPLC iya kuqhubeka nokwandisa kwimizamo exhasayo ejolise ekukhuseleni impilo yezizukulwana ezizayo.