Ngaba uyazi malunga nonyango lwe-vilanity vial vial?

I-slilansung ye-vials ye-HPLCNgaba inkqubo ebaluleke kakhulu eyenzelwe ukuphucula ukusebenza kwe-chromatographic ngokunciphisa umlinganiso wohlalutyo kumphezulu weglasi. Olu nyango luyingenelo ngakumbi ngohlalutyo lobungakanani, ngakumbi xa ujongene neesampulu ezinoxinzelelo, njengoko nayiphi na isampulu yendawo ekhuselweyo inokukuchaphazela kakhulu iziphumo. Eli nqaku libonelela ngamagqabantshintshi ngokubanzi ngenkqubo yokuchasa, izibonelelo, izicelo, kunye nokuqwalaselwa kokusetyenziswa kwiNtloko ye-Chromatography (HPPC).

Yintoni i-shilan?

I-Sulancisization ibandakanya ukusebenzisa i-silane iqulathe kumphezulu wangaphakathi we-borosililicate yeglasi. Le nkqubo isebenza ngokufanelekileyo i-hydroxyl yasimahla ye-hydroxyl (i-SILAnol) kumphezulu weglasi, ezaziwa ukuba zinxibelelana neentlobo ngeentlobo zohlalutyi, ngakumbi iimolekyuli. Ngokuzimasa ezi ndawo ezisebenzayo, i-slilancisation inciphisa i-adsorption yohlalutyo kwiindonga ze-Vial, ngaloo ndlela kuqinisekisa ukuphinda uphinde kunye neziphumo ezichanekileyo ngokuchanekileyo. Kwi-vialc vials, olu nyango lujolise ikakhulu i-Silanol (i-oh-oh) kumphezulu wangaphakathi weglasi ye-borosulicate. La maqela e-hydroxyl akwazi ukunxibelelana nohlalutyili obomvu, okhokelela kwiSampulkyisiwe kunye nelahleko ngexesha lohlalutyo.

Inkqubo ye-slilancisayo

Inkqubo ye-slilancisization ngokubanzi ibandakanya la manyathelo alandelayo:

Ukucocwa: Ukucoca indawo yeglasi usebenzisa i-organic slingven efana ne-acetone ukuze isuse nayiphi na into engcolisayo enokuphazamisa i-slillation.

Ukusetyenziswa kweSilane: Iarhente ye-methyl swille yaziswa kumhlaba ongaphakathi we-vial yiprofayili ye-vapor. Iarhente iphendula ngamaqela e-hydroxyl ukwenza i-hydrophobic ye-hydrophobic engasebenzi kakuhle kwaye ilufumene.

I-Hoving Bound: I-Silane iphendula ngamaqela e-hydroxyl kumphezulu weglasi ukwenza ungqimba lwe-hydrophobic, ukunciphisa indawo yokusebenza kunye nokuthintela uhlalutshelo lweendonga ze-Vial.

Ukuphilisa: Iibhotile eziphathwayo zihlala ziphiliswayo ukuqinisekisa ukuba i-silane yenza iqhina elizinzileyo kumphezulu weglasi, ngaloo ndlela ikhule kwiglasi kunye nezinye iimeko ezihlanganyelweyo ngexesha le-chromatography.

Isiphumo sisiseko somphezulu oguqulweyo esinciphisa unxibelelwano kunye nemilinganiselo ye-polar, ukuphucula ukuphola isampula kunye nokuchaneka kohlalutyo.

Izibonelelo zoShitization

Sebenzisa i-vials ye-HLLC ye-HIPLC ibonelela ngeenzuzo ezibalulekileyo:

1. Ukuphucula ukubuyiswa kwesampulu

I-vials ye-vilawed inokunciphisa kakhulu ukunamathela ekuhlalutyeni kwiindonga ze-vial. Oku kubaluleke kakhulu kubahlalutyo olusezantsi, apho ilahleko yesampunzi yesampulu inokuchaphazela iziphumo zobungakanani. Ngokuthintela i-adsorvor, i-vials ye-slilads iqinisekisa ukuphinda uvuseleleke ngokupheleleyo.

2. Ukuphucula ukuchaneka

Olu nyango luqinisekisa ukuba iziphumo azichaphazelekanga yisampulu yentsalela ukubambelela kumphezulu we-Vial, ngaloo ndlela kuphucule ukuchaneka kombindi olubalulekileyo kuhlalutyo lobungakanani apho kuchaniswa khona.

I-3. Ukuhambelana nohlalutyo ezahlukeneyo

Ii-vials ze-slanded zilungele ukusetyenziswa kwezicelo ezahlukeneyo, kubandakanya ezo zibandakanya iingxondorha zepolar kunye ne-hydrophobic. Zilungele ngokukodwa amayeza, ii-biopolymers kunye nezinye izinto ezinobuthathaka ezinokuthi zisebenzisane ngokubi kunye neglasi yeglasi.

4. Ubomi obude baseShelsi

Inkqubo yenqanaba le-vapor ivelisa iingubo ezizinzileyo ezigcina iipropathi zabo ngokuhamba kwexesha. Iingubo ze-slaaswer zihlala zizinzile ixesha elide nokuba zivezwe kwiinyibilikisi ezahlukeneyo kwaye zilungele ukugcinwa kwexesha elide.

Izicelo ze-vials ye-HILLC

I-vials ye-HPLC ye-HILLCS inokusetyenziswa kwimimandla eyahlukeneyo:

1. Uhlalutyo lweziyobisi

Kwilebhu yasechisa amachiza, i-vials ye-SILDES isetyenziselwa:

Uvavanyo lokuzinza: Ukuqinisekisa ukuba ukwenziwa kwamachiza kuhlala kuzinza ngexesha lokugcina kunye nohlalutyo.

Uhlalutyo lobungakanani: Inceda ukulinganisa ngokuchanekileyo i-concertion yeziyobisi ekuqulunqweni.

I-2. Ukuvavanywa kokusingqongileyo

Ezi vils zibalulekile ekujongesweni kwezendalo:

Ukuvavanywa komgangatho wamanzi: Hlalutya iisampulu zamanzi ezingcolisayo kwi-onmanants ngelixa kuncitshiswa ilahleko ye-adsorction.

Umhlaba uphumeza uhlalutyo: Lungiselela umhlaba ukhupha uhlalutyo lwekhemikhali ngaphandle kokuphulukana nohlalutyo.

I-3. Uvavanyo lokhuseleko lokutya

Kwindlela yokhuseleko lokutya:

Uhlalutyo lwentsalela ye-spistiade

Ukuvavanywa komxholo

4. Uphando kunye nophuhliso

Kwisimo sophando:

Ukulungiselela kwesampulu: ibonelela ngezisombululo zokugcina ezichanekileyo kwiisampulu ezahlukeneyo ngaphambi kohlalutyo.

Izicelo zovavanyo: Ukuququzelela iimvavanyo ezibandakanya iikhawuntariya ezinobuthathaka ezifuna iimeko ze-InErt.

Amanyathelo okhuseleko xa usebenzisa i-vials ye-slial

Ngelixa i-vis ye-cillated inika izibonelelo ezininzi, kukho izinto ezithile ezibalulekileyo ekufuneka ziqaphele:

1. Khetha isampulu ye-BOTLE yeBhotile

Kubalulekile ukukhetha uhlobo oluchanekileyo lwe-vial vial ye-SILLISH yeemfuno zakho zesicelo. Izinto ezifana nevolumu yesampula, ulungelelwano lwekhemikhali, kwaye imvakalelo oyifunayo kufuneka ikhokele ekukhetheni.

2. Izilumkiso zokusetyenziswa

Unyango olufanelekileyo lubalulekile ekugcineni ukuthembeka komphezulu we-shilad:

Kulumkele ukunxibelelana ngqo nendawo yangaphakathi yokuthintela ungcoliseko.

Nxiba iigloves ezicocekileyo xa uphatha ii-vials ukunciphisa ukutshintshelwa kweentsalela.

I-3. Ukuhambelana nezinyibilikisi

Ngelixa iindawo ezingafunekiyo zihlala zixhathisa ngokubanzi kwizinyithi ezininzi, kubalulekile ukuqinisekisa ukuhambelana neekhemikhali ezithile ezisetyenziswa kuhlalutyo lokuthintela ukonakaliswa okanye ilahleko yokusebenza.

Olunye unyango

Ngelixa i-slillaishing iyasebenza, kukho ezinye iindlela ezifana nokuncitshiswa komgangatho womgangatho (000). I-vials ye-RSA yenzelwe ukuba ibe nomsebenzi ophantsi wendalo kwaye ungaxhomekeki kwimali ye-silane. Le ndlela inokubonelela ngeziphumo ezingaguqukiyo kwizicelo ezithile apho i-sulling yemveli inokusilela ngenxa yokugubungela okungaphelelanga okanye ukonakaliswa kwexesha.

Ukuba ufuna ukwazi ngakumbi malunga ne-vials ye-LILLCS, nceda ucofe eli nqaku:"Inkcazo-ntetho ye-vials ye-LILPC"

I-slilansus ye-vils ye-HPLC yinkqubo ebalulekileyo ephucula intsebenzo yohlalutyo ngokunciphisa uhlalutye uhlambelo kunye nokwanda kwesampulu. Ngokuguqula umphezulu wangaphakathi we-vials yeglasi ye-borossilicate, olu nyango luqinisekisa iziphumo ezichanekileyo, ezinokuthenjwa kwizicelo ezahlukeneyo kusetyenziswa amayeza, uvavanyo lwendalo esingqongileyo, ukhuseleko lokutya, kunye noSeto loPhando. Ngokuqonda izibonelelo kunye nokusetyenziswa ngokufanelekileyo okunxulumene ne-vials ye-slilads, iilebhu zenza ngokufanelekileyo i-rolowfilic yokuhlalisa uhlalutya ngelixa ugcina imigangatho ephezulu kunye nokuchaneka kweziphumo. Njengoko iTekhnoloji ye-slicisotion iyaqhubeka ukuqhubela phambili, abaphandi banokulindela ukuphuculwa okuqhubekayo kwintsebenzo ye-Vial kunye nokusetyenziswa kwezinto ezahlukeneyo zenzululwazi.