Ngaba uyazi ngephepha le-chromatography?

IPromatography yePromatography yindlela yokuhlalutya ngokubanzi eyahlulahlula kwaye ichonge imixube yezinto ezisekwe kwizinto ezifanayo zenqanaba lesikhululo (iphepha) kunye nenqanaba leselula (i-Solvent). Kuluncedo kakhulu kwi-chemistry kunye ne-biochemists ekuhlalutyeni inani elincinci lezinto ezinjengemigangatho, i-amino acid, kunye nezinye izinto eziphilayo.

Yintoni i-chromatography yephepha?

Iphepha le-chromatography yephepha luhlobo lwe-chromatography ye-chromatography apho iSigaba seSikhululo sephepha lePhepha leFilint kunye neSigaba seSoft yindlela yokuhamba kwi-slitter pad. Ubuchwephesha buxhomekeke kwisahlulelo sohlukeneyo phakathi kwenqanaba lesikhululo (iphepha lokucoca) kunye nenqanaba leselula (i-Solvent). Njengoko inyibiliki inyusa iphepha lokucoca ngesenzo se-capiter, ithwala izinto zomxube ngamanqanaba ahlukeneyo ngokusekelwe kwizigaba ezibini.

Imigaqo esisiseko esemva kwe-chromatography inokuqondwa ngamagama amabini aphambili: Ukwahlula i-chromatography kunye ne-adsorption chromatography.

Ukwahlula i-chromatography: kolu hlobo, izinto zisasazwa phakathi kwezigaba ezibini ezingamanzi. Inqanaba lesikhululo linamanzi kwi-pores yephepha lokucoca, ngelixa isigaba seSolvent siyinyibilikisi ehamba kwezi pores. Ukwahlukana kwenzeka kuba amakhonkco ahlukeneyo anezinto ezahlukeneyo kwizigaba zesikhululo kunye nesoftware.

I-adsorption chromatography: Apha, umhlaba oqinileyo wephepha isebenza njengenqanaba lesikhululo kunye ne-solvent ye-solvent ye-slitvent isebenza njengesigaba sefowuni. Izinto ezikumxube zahlulwe ngokusekwe kwi-adsorvourter kumhlaba oqinileyo kunye nezomoya zabo kwisigaba solwelo.

Izinto

Isigaba seSitishi: Iphepha leCharomatographic, lihlala lenziwe nge-sellyulose. Inika umphezulu kwizinto ezihambelana nazo.

Isigaba seSoftware: Isinyibilikisi okanye umxube weminyitholi ehamba ephepheni ngesenzo se-capillary. Ukhetho lwe-solvent luchaphazela kakhulu inkqubo yokwahlulahlula.

Isampulu: Umxube wokuhlalutya, udla ngokubonakala kwindawo echongiweyo ephepheni.

Ufuna ukwazi ngakumbi malunga nokulungiselela isampulu ye-HPLC, nceda ujonge eli nqaku: I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo

Inkqubo yePromatography yePromatography

Ukulungiselela: Khetha iphepha lokucoca eliphezulu elinomgangatho ophezulu onesifo esithile sokuqinisekisa ukwahlukana okusebenzayo. Faka isicelo senxalenye encinci yomxube wesampulu kwisiseko sephepha leChromatography.

Ukulungiselela kwesampulu: nyibilikisa umxube wesampulu kwi-solvent efanelekileyo njengenxalenye yesigaba sefowuni.

Ibala: Sebenzisa i-capillary okanye i-micropipette ukulahla isixa esincinci sesisombululo sesampulu kwisiseko sephepha lokucoca.

Uphuhliso: Dipha umda osezantsi wephepha lovavanyo kwisinyibilikisi esiphuhlisiweyo (iSigaba seSoftware). Njengoko inyathelo le-capillary yenzeka, inyibiliki inyuka ngephepha lovavanyo, ithwele iisampulu zesampulu kunye nayo.

Ukwahlulwa: Njengoko inyikima ihamba, izinto ezahlukeneyo zesampulu ziya kuhamba ngamaxabiso ahlukeneyo ngenxa yokusebenzisana kwabo nenqanaba lesikhululo kunye ne-solional smolungulity kwiSigaba seSoloko.

Ukubonwa: emva kokuba umphambili we-Solvent ushukumise umgama owaneleyo, iphepha liyasuswa kwi-solvent kwaye ishiywe ukuba yome. Izinto zinokubonwa zisetyenziswa ngokukhanya kwe-UV, ukuphinda kwenziwe ngemichiza, okanye ngokujonga utshintsho lombala.

Uhlalutyo: Umgama ohanjiweyo kwicandelo ngalinye kwi-SOLPT ngaphambili lilinganiswe ukubala ixabiso le-RF (i-AFTE) yokuchonga.

Izicelo zephepha le-chromatography

IPromatography yePromatography inoluhlu olubanzi lwezicelo kwimimandla eyahlukeneyo:

I-Biochemists: Uhlalutyo lwee-Amino Acids, iSuyars, kunye nezinye ii-biomolecules.

Amayeza: Ukuchongwa kwezinto ezisebenzayo kunye nokungcola kwizinto zokwenziwa kwamachiza. Isetyenziselwa ukuchonga imitya esebenzayo kunye nokungcola kwizinto zokwenziwa kwamachiza ngexesha lophuhliso kunye nolawulo oluphambili.

Inzululwazi yezeNdalo: Iphepha le-chromatography lisetyenziselwa ukufumana amanxuwa kwiisampulu zamanzi kunye nokuhlalutya ungcoliseko emhlabeni.

Ishishini lokutya: Indlela yokusebenza inceda ekuhlalutyeni izongezelelo, ezongcoliseko, kunye neencwadi zokutya ukuqinisekisa ukuthotyelwa kwemigaqo yokhuseleko.

Izinto eziluncedo kwi-chromatography yephepha

Ukusebenza kweendleko: Ifuna izixhobo ezincinci kunye nezixhobo xa zithelekiswa nezinye iindlela ze-chromatographic ezifana ne-HPLC (i-chromatography ye-chromatography). Ukulula: Inkqubo ilula kakhulu kwaye inokwenziwa kunye nezixhobo zelebhu ezisisiseko, ke zinokusetyenziselwa iinjongo zemfundo.

Imfuno yeMuyithi yePlaysi yePlayithi: Ngaba isixa esincinci sesampulu siyadingeka ukuze sihlalutyo, sisenza ilungele iisampulu ezixabisekileyo okanye ezilinganiselweyo.

Ukusetyenziswa uqhelana

Ukulinganiselwa kwe-chromatography yephepha

Ngaphandle kwezibonelelo zayo, i-chromatography yephepha ineentsilelo ezithile:

Umthamo omncinci: ilungele ngokubanzi imiqulu emincinci yesampula.

Isisombululo: Isigqibo sisenokungapheli kangangoko sifezekiswe ziindlela eziphambili ze-HLLC okanye i-chromatography (GC).

Ukuchitha ixesha: Ngenxa yexabiso lokufuduka kancinci, inkqubo inokuthatha ixesha elide ngokuthelekiswa nezinye iindlela ze-chromatographic.

Uhlalutyo olulinganiselweyo: Ngelixa iziphumo ezifanelekileyo zinokufunyanwa ngokulula, iindlela ezongezelelekileyo zokulinganisa zinokucelwa ukuba zithambekele.

Iphepha le-chromatography yephepha lihlala liyindlela ebalulekileyo yokwahlula kunye nokuhlalutya imixube kwimimandla eyahlukeneyo yesayensi. Ukulula kwayo, ukusebenza kweendleko, kunye nokukwazi ukubonelela ngeziphumo ezinomdla kuyenza isixhobo esibalulekileyo kwii-scomists kunye nabaphandi. Ngaphandle kokusikelwa umda xa kuthelekiswa neendlela eziphambili ze-chiromatographic eziphezulu ngakumbi, kusekwe ngokubanzi ngeenjongo zemfundo kunye nohlalutyo lwangaphambili kwilebhu.

Ufuna ukwazi iimpendulo ezingama-50 malunga nee-vials vials, nceda ujonge eli nqaku: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL