I-Chromatography yindlela yokuhlalutya igalelo elisetyenziswa kwiilebhu zelebhu yezicelo ezahlukeneyo zohlalutyo, kubandakanya nokuchonga, ukwahlula, kunye nokudibanisa umxube wemichiza. Ukuqinisekisa iziphumo ezichanekileyo nezinokuthenjwa, ukukhetha i-18mm Vials-ubukhulu obuqhelekileyo kwizicelo zeLebhu-ibalulekile. Apha sikhangela iingcinga ezintathu eziphambili xa ukhetha ubungakanani be-vial.

I-1. Izixhobo kunye nokuhambelana nemichiza

Ukuqwalaselwa kwezixhobo xa ukhethaI-18mm chromatography ye-vialsyenye yezona zibalulekileyo. Ukhetho oluqhelekileyo lubandakanya iglasi kunye neplastiki ezahlukeneyo njenge-polyproplene kunye ne-polythylene; Xa ukhetha i-vials kuvavanyo lwakho lukwabalulekile ukuba balingane neesampulu kunye nezinyibilikisi ezisetyenzisiweyo.

I-vials yeglasi:I-vials yeglasizilungele ukusetyenziswa kwezicelo ezibandakanya iisampulu zobushushu obuphezulu okanye izinyibilikisi ezinoburharha, njengoko zine-intert kwaye zinokuyinyamezela ubushushu obahlukeneyo kunye neemeko zokusingqongileyo. Banokuqhuba kwakhona kwimiba yeplastiki eyenza ukuba ilahlwe lula.

Vials zeplastiki:I-vials yeplastikiUKUFAKWA KOKWENZA IZENZO ZOKUGQIBELA NOKUZIPHATHA NGOKUGQIBELA, Ubenze ukhetho olunomdla kwizicelo ezithile. Nangona kunjalo, abaphandi kufuneka baqiniseke ngeplastiki yabo ekhethiweyo bayodwa ukuba bahambelane nezinyibilikisi okanye iisampulu ezisetyenziswayo, ukuthintela ukunikezela okanye ukungcolisa iisampulu kunye nezinyibilikisi.

I-Solvent-Systent Systix ye-Matrix

Uhlobo lwePolymer	I-PH Uluhlu	Uxinzelelo lokunyamezelana	Ukusetyenziswa okhethekileyo
I-Polypropylene (PP)	1-12	I-600 bar	Izigaba ze-LC-MS ze-IOI ze-ioni-readgents (0.1% tfa)
I-Polyseretothertotone (i-peek)	0-14	> I-1000 Bar	Iinkqubo ze-Uhplc (i-acetonitrile yokudumba <0.5% ngaphezulu kwe-72h)
I-Poriment eguqulweyo	N / A	I-400 bar	Uhlalutyo lwe-Peptide (20-30% yokuphucula ukubuyisela i-vs enganyangwa)

2. Ukuhambelana phakathi kwe-cap kunye ne-septa

Cap kunye ne-septaUkhetho lubalulekile kwi-18mm ye-vialsgraphycy ye-vials njengokhetho lwezinto ezibonakalayo. Basebenza njengezithintelo ngokuchasene nongcoliseko lwesampulu kunye nokuvela kwamanzi, ngoko khawuziqwalasele ezi zinto ngaphambi kokuba wenze ukhetho:

Iindidi ze-cap: Kukho iintlobo ezahlukeneyo ze-cap, ezinjenge-SCICW, i-crmimp caps kunye ne-snap cheps, ezinokulingana nezixhobo zakho zelebhu kunye neendlela zakho zelebhu. Beka phambili ukungangqinelani xa ukhetha enye ukuba isetyenziswe.

I-Septa Stower: I-Septa idla ngokwakhiwa kusetyenziswa izixhobo ezinjenge-polytetrafliothylene (PTFE) okanye i-PTFE) okanye i-silicone, ke ngoko uqiniseke ukuba umntu okhutshelweyo ukuthoba iisampulu kunye nezicoci oceba ukuzivavanya. I-PTFE ibonelela ngokungavisisani kweekhemikhali zekhemikhali ngelixa isiliti se-silicone sibonelela ngezinto ezinokuphononongwa.

3. Izicelo kwaye usebenzise amatyala

Isicelo kwaye sisebenzise iXengulo ziiNgcingayo ezimbini Qaphela:

Ithamo yesampulu: Xa ugcwalisa i-vials enesampulu yohlalutyo, uqinisekile ukuba bafumana ivolumu yakho yesampulu yesampulu. Ukugqobhoza okanye ukuqhubela phambili kwengqondo kunokuphawula ukuchaneka kweziphumo ze-chromatographic iziphumo.

Indlela yohlalutyo: Iindlela ezahlukeneyo ze-chromatographic, ezinjenge-chromatography yegesi (i-GC) kunye ne-chromatography (LC), zifuna iintlobo ezithile ze-vial okanye zixhobo; Qaphela iimfuno zakho ezikhethiweyo ngaphambi kokuba uqhubeke.

Uhlobo lwesampulu

Ukutywinwa kweemfuno: Qwalasela ukuba isicelo sakho sifuna ukuba i-airtight litywina ukuthintela isampulu okanye ungcoliseko.

Xa ukhetha i-18mm ye-Vials Vials, abaphandi kufuneka baqwalasele ngononophelo ngokuhambelana kwempahla, i-cap kunye nokukhethwa kweSepta kunye nokusebenzisa amatyala athile ukufumana izikhongozeli ezifanelekileyo. Ngokunikela ingqalelo kwezi zinto, abaphandi banokuqinisekisa ukuba iimvavanyo zabo ze-chromatographic zenziwa ngokuchanekileyo nangokuchaneka, ukuthembeka, ukuvelisa iziphumo ezithembakeleyo.

Jonga izibonelelo zokusebenzisa i-vials ze-chromatography eli nqaku:Kutheni le nto i-villa ye-ERPE isetyenziswa kwiChromatography? Ii-Angles ezili-12

Ngaphambili:

Amaxabiso athengiweyo kwi-vialsgraphy ye-chiatography yamaziko ophando

Okulandelayo:

I-vialsgraphy ye-chromatography yovavanyo lwetyhefu yetyhefu