I-chromatography yindlela eyandayo yokwanda kwi-chemistry ye-chemistry esebenzisa ukwahlulwa kunye nohlalutyo lomxube ontsonkothileyo. Impumelelo yeenkqubo zeChromatographic ixhomekeke kwizinto ezahlukeneyo; Elinye icandelo elililo yi-vial vial vial, edlala indima ebalulekileyo ekugcineni ingqibelelo yesampulu kwaye ivelise iziphumo ezichanekileyo. Apha sikhangela konke okufuneka ukwazi malunga nezixhobo ezisetyenziswa kwezi zikhongozeli kwaye uqinisekise iziphumo ezichanekileyo.

I-1. Ii-vials zeglasi

I-vials ye-hplcKudala kukuzikhethela kwiilebhu ngenxa yokunganyangeki kweekhemikhali kunye nokungazithembi. I-Borossililicicate iglasi, enye inketho edumileyo, ibonakalisa ukungasebenzi okukhethekileyo kokwandiswa okwenza ukuba kube lula ukulungiselela ukothusa amaqondo omhlaba okanye uhlalutyo oluxhomekeke kwidatha yobushushu. Ngaphaya koko, ukuzinza kuka-Bororilicate kuqinisekisa ukuba ayizukufumana ukungaziphathi kakuhle kwiinkqubo zokwahlukana njengokwahlulwa kwe-chromatographic; Iglasi ye-soda ye-soda isenokubonelela ngokuchaseneyo kancinci ngamachizane.

Hlalani ekuphakameni kwe-chromatography yeglasi ngaphezulu kwe-vialpart. Fumana iimpendulo kwinqaku lethu leSigqibo esiziyo:Izizathu ezi-3 eziphezulu zokuba kutheni iGlasi chromatography ye-vials ilunge ngakumbi kune-vials yeplastiki

I-2. Ii-vials zeplastiki

I-vials yeplastiki yeplastikiUzuze ukuthandwa ngenxa yendleko zabo zokuxabisa, indalo ekhanyayo kunye nokuchithwa. I-Polypropylene kunye nePlasthics zePolithylene zikhetha zokhetho xa zivelisa i-vials zeplastiki zeplastiki zeplastiki; Zombini zinokunika izibonelelo xa kufikwa ekunikezeleni ukhuseleko lwee-ion zentsimbi ukusuka kwisisombululo, ezibenza bazilungele ezinye iinkqubo; Nangona kunjalo kufuneka zisetyenziswe ngononophelo xa zisetyenziswa kumaqondo obushushu aphakamileyo njengoko ezinye iiplastiki zisenokungakwazi ukumelana namaqondo obushushu.

Fumanisa izizathu zokukhetha i-vialsgraphy yeplastiki yeplastiki. Dive kwinqaku lethu lokuqonda kwesi sigqibo sohlalutyo esibalulekileyo:Iinzuzo ezi-4 zokusebenzisa i-vialsgraphy yeplastiki yeplastiki

I-3. Polytetrafluoroothyyyyyyyyyyey (Ptfe)

Ingubo ye-PTFO idlala indima ebalulekileyo ekunciphiseni ukudityaniswa kwesampulu kunye nendawo ye-vial vem. Iipropathi zabo ezingezolomangazi zinciphisa uhlaziyo kwiindonga ze-Vial, ngaloo ndlela zinciphisa ukulahleka okunokwenzeka nokuqinisekisa ubungakanani obuchanekileyo. Eli nqaku libaluleke ngokukodwa xa ujongene nohlalutyo lwenqanaba lokudibana apho kude kufike khona naphantsi kwemizuzu yohlalutyi-mfundo inokubakho kwi-ramatic fition kwiziphumo; ngenxa yokoI-vials edityanisiweyoUkunyusa ukuthembeka kunye nobuntununtunu ngexesha lemilinganiselo yeCromatographic.

I-4. Silicone Septa

I-Septa, okanye i-caps, eyenziwe nge-silicone ifumaneka kwi-vialsgraphy yeChromatography ngenxa yendalo, ukuguquguquka kunye nokuqina. USilicone ubonelela ngetywina elinokuthenjwa eligcina ungcoliseko ngelixa ekhusela iisampulu ngokuchasene ne-Evaporation ngexesha lenkqubo yohlalutyo - zombini ezi zinto zibalulekileyo ekugcineni ingqibelelo. Iipropathi zabo ze-elastic zenza ukuba ukusetyenziswa okuphindaphindiweyo ngelixa kusekho itywina le-airtight, ukongeza ixesha elide kwi-chromatography yeenkqubo ngokubanzi.

Ukufuna ulwazi olupheleleyo kwi-ptfe \ / sisilicone septa? Jonga ngakumbi! Jonga inqaku lethu ukuze siqonde kakuhle ezi zinto zibalulekileyo:Yonke into oyifunayo

I-5. Ubungakanani be-vial kunye nobume

I-vialsgraphy yeChromatography ingena ngobukhulu obahlukeneyo kunye nemilo yokudibana neemfuno ezahlukeneyo zohlalutyo. Ubungakanani obuqhelekileyo malunga nohlalutyo lweziqhelo luyi-1.5 ml kunye ne-2 ml vials, nangona i-vials enkulu okanye incinci inokukhethwa kuxhomekeka kwivolumu yesampulu kunye neemfuno ezithile zohlalutyo. Ii-vics ezibonisa iziseko ezinomsindo okanye zeflethi zinempembelelo yefuthe kwisampulu amazinga e-Eavaporation-isebenza ngempumelelo kwiinkqubo zokwahlula-hlula-hamba ngenjongo yokwahlulahlula umsebenzi wokwahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulo.

6. Isiqinisekiso kunye nolawulo lomgangatho

Isiqinisekiso seI-HPLC Vialsyinto ebalulekileyo ekuqinisekiseni ukufaneleka kwabo ekusebenziseni ukusetyenziswa. Imizimba esebenzayo enjenge-United States Pharmacopeia (i-USP) kunye ne-European Pharmacopea (EP) (EP) (EP) IMilinganiselo yoLawulo lweMpahla, ngokunika abaphandi ngokubhekisele kwimiphumo yohlalutyo oluthembekileyo.

Dive kwinqaku lethu ukufumana izicelo ezili-15 ze-vialsgraphyy. Vula uqwalaselo lwezixhobo ezibalulekileyo zohlalutyo oluchanekileyo:I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

Kwilizwe elinobunzima be-chromatography, izixhobo ezisetyenziswa kwi-Vial Yomdlalo Dlala indima ebalulekileyo kwimpumelelo yayo. Iglasi kunye ne-vials yeplastiki, i-Ptfeeds, kwayeI-Silicone SeptaNonke inikezela ipropathi ethile eqinisekileyo yokuphucula ukuchaneka, ubuntununtunu, kunye nokuthembeka ngexesha lokuhlalutywa. Njengabaphandi bahambise ukhetho lwabo phakathi kwabo bonke, ukuqonda ezi ndawo zixhobo zezinto kunye nezicelo ziphambili ekukhetheni ii-vials ezifanelekileyo- nokuba yiglasi ye-Exgic ekuchaphazeni ixesha elide okanye ukusetyenziswa kweplastiki yokusetyenziswa kweplastiki. Abaphandi banokwenza izigqibo ezinolwazi ezinokuba neziphumo ezizayo kwiziphumo ezinempembelelo kwimisebenzi yazo yohlalutyo.

Vula ii-Insts ezingama-50 kwi-vials ye-HPPL! Jonga inqaku elibanzi elibanzi leempendulo kwimibuzo yakho evuthayo:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Kutheni i-crimpp i-vials ephezulu isetyenziswa kwi-chromatography? Izizathu ezi-5

Okulandelayo:

Izizathu ezili-10 Kutheni kufuneka usebenzise i-pthofey ye-chiatography ye-chials