I-chromatography yindlela yokuhlalutya igalelo elisetyenziswa kumacandelo amaninzi kubandakanya amabala amachiza, uhlalutyo lokusingqongileyo, kunye novavanyo lokutya lokutya. Ukufezekisa iziphumo ezichanekileyo nezibhengezileyo kubalulekile ukukhetha izinto ezifanelekileyo zeChromatography yakho - i-vision enye ihlala ikhethwa i-mil-faks eneemifanekiso ze-micros ukuze zikukhokele le nkqubo.

Ukuqonda indima yokufaka i-Micro

I-micro-fakaNgaba izixhobo ezincinci zeglasi okanye izixhobo ze-polymeric ezenzelwe ukulingana ngaphakathi kwentamo ye-vialsgraphy. Injongo yabo kukunciphisa indawo yomphezulu phakathi kwesampulu yakho kunye nengaphakathi yayo malunga nokunciphisa umngcipheko wesampulu yesampulu kunye nongcoliseko.

Ukufaka i-Micro-faka ukudlala indima ebalulekileyo ekugcineni ukuthembeka kweesampulu zakho. Ngokuncipha kwendawo yoqhakamshelwano phakathi kwesampulu kunye ne-vial, banciphisa amathuba okuba uhlaluthwe iindonga ze-Vial, ezinokubangela iziphumo ezichanekileyo okanye ilahleko yesampulu. Ukhetho lwakho lwezinto ezibonakalayo kunye noyilo lwe-Micro-nume lunokuba nefuthe elikhulu kakhulu kwiziphumo zohlalutyo lweChromatographic.

Cinga ivolumu yakho yesampulu

Into yokuqala xa ukhetha i-micro-fin kufuneka ibe yimozulu yayo yesampula. I-Micro-Pleints iza ngobungakanani obahlukeneyo ukusukaI-100 il ukuya kwi-400 il; Ke ngoko kubalulekile ukuba nayiphi na into encinci ekhethiweyo inokuhlalisa isampulu yakho ngaphandle kokuphelisa okanye ukushiya indawo enkulu kakhulu.

Ukukhetha umthamo ofanelekileyo ofanelekileyo unokunciphisa inkunkuma yesampulu kunye nokuqinisekisa uhlalutyo oluchanekileyo. Ukukhetha kakhulu ifrafti enkulu kakhulu yesampulu yakho kungakhokelela ekugcinweni ngaphakathi kwi-daltare. Ngelixa ukhetha incinci kakhulu kunokuthetha ukuba ayinakukwazi ngokufanelekileyo isampulu yakho.

Khetha izinto ezifanelekileyo

I-micro-fakayiza ngeendlela ezahlukeneyo, ezinjengeglasi kunye ne-polymers njengePolypropylene kunye ne-ptfe. Iglasi ifaka i-Micro-i-Microuls idla ngokuba yi-Intert kwaye ilungele uninzi lwezicelo, nangona ibuthathaka. Kwelinye icala, i-polymer mic-fatha i-Micro-iflot zihlala zihlala ixesha elide kuneglasi yeglasi yazo ngelixa inika umngcipheko ongaphantsi kophule kodwa unganikezeli njengabaninzi.

Ukukhetha kwakho izinto ezibonakalayo kuxhomekeke kwiisampulu zakho kunye nezinyibilikisi; I-Glass-pumels inokuba yeyona nto ilunge kakhulu kwizinyibilikisi okanye iisampulu ezifuna ukungabikho ngokupheleleyo; I-polymer micro-faka i-Micro-faka ezinye iindlela zokuxabisa ezisebenzayo ezinokusebenza.

Tshatisa i-Micro-pieff yakho kunye ne-vial yakho ayiyiyo yonke i-micro-ufaka ifanelwe yonke i-vial; Ukuqinisekisa ukulunga, tshatisa ubungakanani be-Micro-faka kwintamo yayo ngokubhekisele kwi-diameter-i-Microm-factos yenzelwe ukuba i-9mm okanye i-11mm i-onms ukuze isebenzise ubungakanani obufanelekileyo.

I-Mismatches phakathi kwe-micro-faka kunye nesayizi ye-Vial inokukhokelela ekuvusweni okanye i-ungcoliso yesampulu elalanisa ngokuchaneka kweziphumo ze-chromatographis, ke kubalulekile ukuba zitshintshe ukuthembeka kovavanyo lwakho.

Qwalasela i-Micro-puses ekhethekileyo

Kukho i-Micro-pumes ekhethekileyo eyilelwe ngokukodwa isicelo sakho. Umzekelo, xa usebenza neesampulu eziguqukayo Kububulumko ukukhetha ukufakwa kufakwe kwiglasi eyakhiwe ngeglasi eyakhiwe ngeglasi okanye i-ptfes kwi-vials kwii-vials ngokufanelekileyo kwaye kugcinwe ingqibelelo yesampulu ngexesha uhlalutyo. Oku kufaka uncedo lokukhusela ingqibelelo yesampulu kwaye kugcinwe intembeko yeenkqubo zokuhlalutya.

Kwiisampulu zithambekele ekugxothweni, khetha i-Micro-ifaka i-Micro-faka abathi boMbuso njengoko oku kunciphisa ukuvezwa kwesampulu yakho kunye nokunciphisa nawuphi na umngcipheko wokuphulukana nempumlo. I-Micro-PIRS ekhethekileyo ye-Micro-i-Micro-i-Micro-i-Micro-i-Micro-i-MicroPOS kwizicelo ezithile zezinto ezichanekileyo nezinokuthenjwa kwiimvavanyo zakho zeChromatographic.

Ukuvavanywa kokungqinelana

Ngaphambi kokwenza ukhetho lwakho lokugqibela, kububulumko ukuqhuba uvavanyo lokuhambelana nakweyiphi nai-micro-fakaUcinga ukusebenzisa inkqubo yakho yeChromatographic yakho. Oku kubandakanya ukuzivavanya kwiisampulu zakho kunye nezinyibilikisiweyo ukuqinisekisa ukuba azisebenzi kakuhle okanye zazise nakuphi na ukungazithembi kwinkqubo yakho yohlalutyo.

Uvavanyo lokuhambelana yinxalenye ebalulekileyo yokuvavanywa ngempumelelo kwaye ikuvumela ukuba uchonge nayiphi na imicimbi ngaphambi kokuba iphazamise iimvavanyo zokwenyani. Ngokuvavanya i-Micro-faka ngokuchasene nesicelo sakho kunye nokuqinisekisa ukuba abayi kulalanisa ukuba banciphise umgangatho okanye ukuchaneka kohlalutyo lwe-chromatographic, uvavanyo lokuhambelana luyanceda ukuba ichaphazele naluphi na umngcipheko ngaphambi kokuba ichaphazele.

Khetha ukufakwa kwegama eliphantsi

Xa usebenza ngemiqulu encinci, i-Micro-faka ivolumu efileyo inokuba luncedo ekunikezeleni isampulu yesampulu ethembekileyo - iluncedo kakhulu xa iphatha iisampulu ezixabisekileyo okanye ezinqabileyo apho zonke itreyini ibala.

I-pumes yomthamo ophantsizilungele ukusetyenziswa apho isampulu bulinganiselwe, njengokuba ujongene ne-chare okanye ebizayo. Ngokunciphisa ivolumu efileyo, ezi kufaka ukwandisa ukubuyela kwakhona kweesampulu ngelixa usenza uninzi lwezixhobo ezinqongopheleyo.

Iinketho zokhetho

Iimfuneko zakho zophando azinakuhlangabezana nezinto zokufaka ezincinci ezincinci, kwaye abavelisi babonelela ngeenkonzo zokugcwalisa ngokwesiko ukuze i-micro-faka i-micros ilungelelaniswe ngokuthe ngqo ukuze idibane nayo.

Ukufakwa kwemveliso ngokwezifiso kunokuba kubaluleke kakhulu xa usebenza neesampulu ezinzima okanye iimeko ezinzima iimeko zeCromatographic. Ukwenza iziko uqinisekisa ukuba i-micro-faka i-micro-faka i-Micro-proces yakho yonke imiboniso yakho ngokuchanekileyo, isebenzela iimvavanyo zakho.

Ixabiso lemali kunye nomgangatho

Ngelixa umgangatho kufuneka uhlale kuqala xa ukhetha i-micro-iport, ukuqwalaselwa kweendleko nako kudlala indima. Vavanya zombini uhlahlo-lwabiwo mali lwakho kunye nokulindelwa kwemali elindelekileyo ngaphambi kokukhetha i-microfayili; Ngamanye amaxesha ukutyala imali kwimigangatho ephezulu yokufaka kunokwenza ingqondo ngelixa ukhetha iimodeli zexabiso eliphantsi lomsebenzi wemihla ngemihla.

Ukulinganisa indleko kunye nomgangatho kwilabhoratri yakho kufuna ukwenza isigqibo esizimeleyo ngokusekwe kwiimfuno zalo ezithile nakwizithintelo zemali. Ngokujonga ngononophelo umyinge onesizuzo, unokwenza izigqibo eziqinisekisa ukuthembeka ngovavanyo ngaphandle kokungaphezulu kokungaphezulu kwezidanga zohlahlo-lwabiwo mali.

Ukulungiswa rhoqo

Nje ukuba uthenge i-micro-pumes, kubalulekile ukuba baba yinxalenye yeNkqubo yeNkqubo yeLabhoratri. Ukucocwa rhoqo kunye nokukhathalela qinisekisa ubuchwephesha kunye nokuqhubeka kokusebenza kwezi kufakwa-nceda thintela imiba engalindelekanga ngexesha lovavanyo.

Ukulungisa rhoqo ngokulondolozwa njengokusebenzisa izinto ezifanelekileyo zokucoca kunye nokuhlola ukunxiba kunye nokukrazula kuyakwandisa ubuntu bakho obufakelayo kwaye kuqinisekiswe ukusebenza ngokungaguqukiyo ngexesha lokuphiliswa kwakho lweChromatographic. Ngokuquka ezo zinto kwiiprothokholi zakho zelebhu, unokwandisa ixesha lokuphila kwabo ngelixa uqinisekisa ukusebenza ngokungaguqukiyo ngokuhamba kwexesha.

Ukuqukumbela

Ukukhetha iI-micro-fapmus efanelekileyoyeyakhoI-chialsgraphy ye-chromatographyisitshixo sokuqinisekisa ukuba uhlalutyo oluchanekileyo noluthembekileyo. Cinga ngezinto ezinjengevolumu yesampula, ukukhetha izinto ezibonakalayo kunye nokwenza ngokweziko xa usenza esi sigqibo sokukhetha. Ukulungiswa rhoqo kunye nokulawulwa komgangatho ukuqhubela phambili ngovavanyo oluphumelelayo, nceda ukufezekisa iziphumo, iziphumo ezibuyisiweyo kwimeko yesayensi okanye yemisebenzi. Ngokwenza ukhetho olunolwazi kunye nokunikela ingqalelo kwiinkcukacha onokukwenza i-SETUP yakho yokuSebenza kwaye ufezekise iziphumo ezilungileyo ezivela kuyo.

Ngaba ufuna ulwazi olunzulu kwi-vial vial vial vial? Jonga eli nqaku libanzi ngolwazi oluthe kratya: I-HPPC vial vial:Ukuphucula ukuchaneka kunye nempazamo yesampulu

Kwaye

Vula iimpendulo kwi-50 imibuzo ebuzwa rhoqo malunga ne-vials ye-HLLC kwinqaku elibanzi nelifundisayo: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Isishwankathelo sokukhetha i-vial sesampulu yamanqaku ali-11

Okulandelayo:

I-chillsgraphy ye-chromatography yohlalutyo lomgangatho wamanzi