I-Chromatography sisixhobo esibalulekileyo kuphando lwesayensi kunye nohlalutyo, ukuvumela ukwahlukana, isazisi, kunye nokudityaniswa komxube ontsonkothileyo. Izicelo zayo zibandakanya amayeza, ukubeka iliso kwindalo esingqongileyo, ii-forensics, kunye nezinye amasimi amaninzi. Kwindlela yokusebenza yeCromatography, ukucoceka kwesixhobo, ngakumbi iibhotile zeChromatography, kubaluleke kakhulu. Ezi zikhongozeli kufuneka zicocwe kakuhle ukuze zisuse iintsalela ezinokuthintela ukuchaneka kunye nokuthembeka kweziphumo zohlalutyo. Ubukho beearhente zokucoca abaphumeleleyo kwezi vileni ezinokwenza izinto ezingafunekiyo ezinokwenza idatha kwaye zikhokelele kwizigqibo eziphosakeleyo.

Isicoci sentsalela

Intsalela eshiyekileyo ngaphakathiI-chialsgraphy ye-chromatographyEmva kwenkqubo yokucoca inokubandakanya iintlobo ezahlukeneyo. Iiarhente zokucoca i-alkaline ezinje nge-sodium hydroxide zihlala zisetyenziselwa ukugqithisa iintsalela, ngelixa izisombululo ze-acidic ezinjenge-hydrochloric acid zinokusetyenziselwa ukususa iidipozithi ze-hydrochloric. Izinyibilikisi ze-organic ezinje nge-methanol kunye ne-ethanol ziyasebenza ngokuchasene nokungcoliseka kwe-nopopu, kunye ne-surfacapes ezinje ngeTriton X-100 zihlala zibandakanyiwe kwifomu yokucoca nokusasaza i-comples. Ezi ziqwengana sentsalela, ukuba azisuswa ngokupheleleyo ngexesha lokuphamba, zinokuhlala kwindawo yebhotile kwaye zinxibelelana nesampulu ngexesha lohlalutyo olulandelayo.

Ngaba ujonge isikhokelo esipheleleyo ekucoceni i-vialsgraphy yesampulu ye-chiatography? Jonga eli nqaku kulwazi olupheleleyo kwiindlela ezifanelekileyo zokucoca kunye nezona ndlela zilandelayo:Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys

Impembelelo kungcoliseko lwesampulu

Impembelelo yearhente yokucoca intsalela kwi-insumlation yesampulu zininzi. Nditsho nezixa-mali zalearhente zinokungcolisa isampulu kwaye zitshintshe ubume bayo. Umzekelo, i-surficapes inokwenza i-adsorb kwimodeli yesampulu kwaye ichaphazele indlela yokuziphatha yohlalutyi, ekhokelela kwiziphumo ezibonakalayo ze-chromatographic. Ukongeza, i-acids entsalela okanye iziseko zinokutshintsha i-PH yesampulu, echaphazela i-ionifmium yokulingana kunye ne-chromatographic ixesha lokugcina. Ungcoliseko olunjalo alubeka emngciphekweni ukuchaneka kohlalutyo lobungakanani, kodwa kukwaphazamisa ukuthembeka kokuchongwa komgangatho.

Iziphumo kwiziphumo zovavanyo

Iziqwengana zentsalela zinokuba neziphumo ezithe ngqo nezingathanga ngqo kwiziphumo zovavanyo lovavanyo lweChromatographic. Impembelelo ethe ngqo kukuphazamiseka ngokwahlulahlulo lwe-chromatographic enokubonakalisa njengokutshintsha kobume be-Peak, kwingxolo yesiseko, okanye utshintsho olungalindelekanga kwixesha lokugcina. Olu phazamiso lungafikelela ipikiti, ukunciphisa ubuntununtunu, kwaye kuthintele ukubongwa kwemiqathango ephantsi. Ukongeza, ubukho beearhente zentsalela zinokukhuthaza iikhemikhali kunye nohlalutyo, ezikhokelela ekuthandeni ukonakaliswa, ukugqwetha ukwakhiwa kwesampulu yesampulu kunye nokutolikwa kwedatha.

Ngaba unomdla wokukhetha i-vial ye-HPPC yakho? Fumanisa ukuba ukhetha njani ilungelo lokusebenza ngokugqibeleleyo kwe-chromatographic:Ndikhetha njani ilungelo lokufaka kwi-vial yam ye-HPPL?

Iqhinga loNyalo

UkuPhuhlisa iindlela zokunciphisa ezomeleleyo kubalulekile ukunciphisa ifuthe learhente yokucoca intsalela kwi-Chromatographic uhlalutyo.

Ukucoca ngokucokisekileyo:Ukuseka i-protocol yokucoca ebanzi ebandakanya ukuhlamba okuqhubekayo kunye namanzi aphumeleleyo okanye isilivere efanelekileyo yokususa ngokupheleleyo isiqwengana sentsalela.

Iiprothokholi zokuqinisekisa:Ukumiliselwa kweenkqubo zokuqinisekisa ezingqongqo ukuqinisekisa ukuba i-uprimus engenanto, iimvavanyo zokuhambelana kwenkqubo, kwaye ulawulo lolawulo lwenziwa ukuqinisekisa ukungabikho kwearhente yentsalela.

Izixhobo ezinikezelweyo:Cinga ngokunikezelwa kweebhotile zeChromatography okanye izixhobo ezinikezelwe kwiindidi ezithile zohlalutyo ukuze unciphise umngcipheko wokungcoliseka komnqamlezo.

Ulawulo lwemeko:Ukuqesha amanyathelo olawulo oluphambili obukhulu njengokutshekishwa kwexesha lokuziqhelanisa nexesha, inaliti engenanto, kunye nemisebenzi esebenzayo ye-Deamations.

I-arhente yearhente yokucoca ngaphakathiI-chialsgraphy ye-chromatographyIbeka umngeni obalulekileyo ekunyanisekeni nasekuthembekeni kohlalutyo lweChromatographic. Ngokuphumeza iiprothokholi zokucoca ezingqongqo, iinkqubo zokuchaza, kunye namanyathelo olawulo lomgangatho, iilebhu zinokunciphisa ifuthe labaxhasi lokucoca kunye neziphumo zovavanyo olugqibeleleyo.

Ngaba ungathanda ukuphonononga inkalo ethile ngokweenkcukacha ngakumbi okanye uxoxe ngesihloko esilandelayo?

Ndinomdla malunga neempendulo ezingama-50 malunga nee-vials ze-HPPL? Dive kule nqaku libanzi ngalo lonke le mibuzo yakho malunga ne-vials ye-chromatography ye-chromatography ye-chials:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Ikamva lokuziqhatha: Itekhnoloji ekhulayo kwi-0.45 yezifiso ze-syringer

Okulandelayo:

Izakhiwo ze-microscopic kwi-vialsgraphy ye-chromatography: ifuthe elinokubakho kwisampulu yogcino kunye nohlalutyo