I-chromatography yindlela ebalulekileyo yokuhlalutya isetyenziswe kwiinkqubo ezininzi zenzululwazi, ukusuka kwi-chemistry kunye namayeza kwiSayensi yezeNdalo kunye nohlalutyo lwendalo. Xa usebenzisa i-chromatography njengenxalenye yenkqubo yayo, i-vials esemgangathweni ephezulu kunye nokuvalwa kubalulekile ekugcineni ingqibelelo yesampulu kwaye uphephe ungcoliseko; Kodwa iilabhoratri zihlala zibuza ukuba ziphinde zancitshiswai-vials kunye nokuvalwaukulalanisa iziphumo kunye nokuchaneka kwedatha; Kweli nqaku siza kuphonononga izinto ezichaphazela olu hlengahlengiso kunye neendlela ezilungileyo zokuphatha kwazo ngokufanelekileyo.

I-Vials kunye nokuvalwa kweChromatography: ukubaluleka kwabo kuhlalutyo

I-vialsgraphy yeChromatography kunye nokuvalwa kusebenza njengezikhongozelo eziphambili zesampulu ngexesha lohlalutyo. Ngenxa yoko, kufuneka bathobele imigaqo-nkqubo yokuqinisekisa ukhuseleko isampulu, bagcine ubunyulu, kwaye bavimba iimpembelelo zangaphandle ekuphazamiseni iziphumo. Iivileni ziza ngezinto ezahlukeneyo ezinje ngeglasi okanye iplastiki ye-intert ukulungiselela iindidi ezahlukeneyo zesampulu kunye neendlela zohlalutyo ngelixa zivala i-vials ngokuchasene ne-vials ngokuchasene nomxholo wabo.

Uninzi lweechemics kunye nabaphandi Buza ukuba I-vialsgraphy ye-chromatography kunye nokuvalwa inokuphinda isetyenziswe emva kokucocwa ngokufanelekileyo I-Laborary Shrewashers(ngakumbi Ukuhlanjwa kweglasi ye-lab).

Ukuyayenzale ibalulekile umbuzo cacisa, ezimbini zeengcali kwi-United States kwaye i-UK iqhube isifundo sophando kwaye yazisa iziphumoyokusebenzisa i-vials ehlanjululwe kwakhona kunye nokuvalwa Kwinqaku lobugcisa 20670.

Fumanisa isikhokelo esibanzi kwimibuzo engama-50 exhaphakileyo ebuzwe malunga ne-vials ye-HPLC kule nqaku inolwazi ne-inzulu: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPLC

CI-ofisi yokusetyenziswa kwehlanjiweyo I-vialsgraphy ye-chromatography kunye nokuvalwa nge-vials entsha usebenzisa irhasicIzixhobo zeHromatography kunye nezixhobo ze-hppc,Bona chazaiingxaki ngemifanekiso emininzi kunye neetafile,nako Ubuninzi bobungakanani be-vials.RNgokunomdla ngokunomdla, TIifoto ze-vials ezisetyenzisiweyo zisebenzise i-vials ezibonisa ii-Glues ze-Glues, ukuphakama kwentambo, kunye nokufakwa kweglasi. Kodwa, umdla onomdla wokwaziswa kwe-100x ibonisa imiphezulu ye-jeyishes ye-vials yeglasi ecacileyo neye-amber ngaphambi kokuba isetyenziswe kwaye emva kokucocwa kwe i-vial esetyenzisiweyo.

Ezinye izigqibo malunga ne-vialsgraphy yeChromatography kunye nokuvalwa zibandakanya ezi zinto zifunyenweyo:

Izinto ezongezelelweyo zafunyanwa kwi-GC-MS Anks kuwo onke amatyala ovavanyo.

Ixesha lokufumana isixhobo se-GC landa ngenxa yenqanaba elonyukayo lokutshintshwa kwentsholongwane.

Inaliti yevolumu encinci encinci ibonise ukungeniswa kwezincopho ezongezelelweyo kwimowudi ye-UV yovavanyo lwesixhobo se-HPPC.

Ungcoliseko lwenaliti ukusuka kwi-vials yanda ngexesha lokusebenzisa i-Septa kunye nelahleko yelahleko.

Kwigama, isincomo sengcali: I-vialsgraphy ye-chromatography kunye nokuvalwa. "

Hlalani ukubaluleka kokusebenzisa i-vials ecacileyo kwi-chromatography kwaye uqonde indima yabo ebaluleke kakhulu ekuqinisekiseni iziphumo ezichanekileyo. Kutheni le nto i-vill ecacileyo ekhethwe kwi-chromatography?

Iimbonakalo ezi-4 ziphembelela ukuphindwa kwee-vials kunye nokuvalwa

Izinto ezininzi zidlala indima ebalulekileyo ekuqinisekiseni ukuba i-vialsgraphy ye-vials kunye nokuvalwa kungasetyenziswa ngokukhuselekileyo:

1. Ukunyaniseka kwempahla

Ukukhetha izinto ze-vial kunye nokuvala zidlala indima ebaluleke kakhulu kwindima yabo. I-vials yeglasi, eyaziwa ngokuba kukungavisisani kwabo nokungavisisani kweekhemikhali, ithande ukuba kungcono ngokuphinda isetyenziswe kwakhona kunezikhala zeplasitiki? Izinto zokuvalwa kwe-intert ezinje nge-ptfe okanye i-silinene ibonelela ngokuphuculwa kokusebenza kwezinto ezininzi.

2. Ukucoca ukusebenza kakuhle
Ukusetyenziswa ngokuphumelelayo kwi-Vial kunye nokuvalwa kwakhona, iinkqubo zokucoca ezifanelekileyo zibalulekile. Ukususwa ngokuchanekileyo kwentsalela yangaphambili yentsalela kunye nokungcola kuqinisekisa uhlalutyo olungafunekiyo, ngelixa iiprothokholi zokucoca kufuneka ziqeshwe iziphumo ezifanelekileyo.

I-3. Iindlela zokubulala i-sterilization

Ukucocwa kubalulekile ukuphepha ungcoliseko lwe-vials kunye nokuvalwa, ukungcungcutheka, ukucocwa kwamachiza okanye ezinye iindlela zokuvunywa kufuneka zisetyenziswe ngokuxhomekeka kwimibutho esebenzayo.

4. Ukufaneleka kwesicelo

Ekugqibeleni, i-vials iphinde yasetyenziswa kwakhona kwaye ukuvalwa kuxhomekeke kakhulu ekusebenziseni kwabo. Ngelixa uhlalutyo oluthile lunokumelana namanqanaba amancinci ongcoliseko kwizikhongozelo zokuchaneka kweziphumo, ezinye izicelo ezibandakanya izitya ezinobuthathaka

Emva koko uhlalutyo olungcono noluchanekileyo,Sebenzisa kwakhonaI-chiatography vials okanye ukuvalwa ayisiyonto elungileyo, nayiphi na imfuneko yeI-chialsgraphy ye-chromatography, ukuvalwa kunye nezinye izinto zinxibelelana nathi.

Ukutyhila iimpendulo kwiMibuzo ePhezulu iPhezulu malunga neMibuzo ye-HLLC kunye nenqaku elibanzi nelifundi: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPLC

Ngaphambili:

Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys

Okulandelayo:

Ngaba uyalazi ngokwenene i-chromatography ye-chromatography ephezulu?