Indlela yokuhlalutya iPuqula, i-chromatography isetyenziswa kwimimandla eyahlukeneyo yesayensi eyahlulahlulwa kunye nokuhlalutya umxube ontsonkothileyo. Kwintsimi yeChromatography, i-vial yinxalenye yesiseko kwaye iSeptum isebenza ukukhusela ukuthembeka kwesampulu. Esi sikhokelo sivaleka kwi-intricaries yeIiplanga ze-vialsgraphys vialskwaye ibonelela ngovavanyo olunzulu lweentlobo zabo, izixhobo kunye nezicelo.

I. Ukuqonda ii-sepgus

I-SEPPUS ingumsipha emva kwe-vim ye-chim yeChromatography kwaye isebenza njengomqobo okhuselayo wokugcina imeko icocekile. Ngokutyhila i-vial, i-sepgus ithintela ungcoliseko oluvela kwimeko yangaphandle kwaye uqiniseke ukuthembeka kweziphumo zohlalutyo. Eli candelo libonisa indima ebalulekileyo ye-sepgoms yokudlala ekugcineni ingqibelelo yesampulu kuyo yonke inkqubo yeChromatographic.

II. I-4 ikakhulu iintlobo zeSeptum

I-Silicone Septum:

Yaziwa ngokuba yixhasi labo lekhemikhali kunye nokuqina, i-Silicone sepum ifumene i-niche kwizicelo ze-chromatography zegesi. Ukuhamba kwabo jikelele kubangela ukuba baphile phantsi kohlalutyo lwemilinganiselo yobubi kwaye igalelo ekuchazeni iziphumo zohlalutyo.

I-ptfe (iPolytetrafluoroothylene) Septum:

I-ptfe sepgusNgaba i-intert ye-camy kunye nokunganyangeki kwizinyithi eziqinileyo. Ezi zixhobo ziluncedo kakhulu kwizicelo ze-chromatography ze-chromatography, ngakumbi xa zijamelene neesampulu zoncedo kunye neendawo ezinzima zemichiza.

I-Subber Septum:

Yenziwe ngezinto ezinje nge-butyuber irabha kunye nerabha yendalo, ii-ruber zerabha zibonelela ngetywina elinokuthenjwa ngeenjongo ze-chromatography ngokubanzi. Uhlobo lwabo luqinisekileyo lubavumela ukuba bafakwe kuluhlu olubanzi lweesampulu kunye neemeko zokuhlalutya.

I-Pre-Slit Septum:

I-Pre-Slit Sept ASPUFS yenzelwe ukungena ngokulula impahla yokungena inciphisa umngcipheko wokulungisa. Ziluncedo kakhulu kwizicelo ezifuna ukufikelela rhoqo kwiisampulu kunye nokuphucula ukusebenza kakuhle kwendlela yokuhamba kohlalutyo.

Ndinomdla wokukhetha phakathi kwe-pre-slit okanye i-plit engaphambi kokuba iphumelele? Dive kwinqaku lethu lokuqonda ngokwenza isigqibo esifanelekileyo seemfuno zakho zeChromatography:Ungayikhetha njani i-Septa Pal-Slit okanye hayi?

III. Ii-3 zohlobo ezisetyenziselwa ii-seples

I-Butyl Ruber:

I-Butyl Ruber Mepranes, iphawuleka ngokuchasene nemichiza elungileyo, ilungele ukutywinwa ngokuchasene nezixhobo ezingezizo ezingoobarlar kunye ne-polar. Oku kubenza babe kukhetho oluthembekileyo kuluhlu olubanzi lwezicelo zeChromatographic.

Isilika

Umdibaniso weI-Silicone kunye ne-ptfeUdibanisa izibonelelo zazo zombini ezi zinto ukubonelela ngokuxhathisa kakhulu kunye nokuqina. Esi sixhobo sezinto ezihlanganisiweyo zinyusa ukuthembeka kweSeptum ekufumaneni iindawo zohlalutyo.

Ndinomdla wokufumana i-Ins kunye nokuphuma kwe-ptfe \ / Silicone Septa? Jonga inqaku lethu elinzulu lokuqonda okupheleleyo kunye nokukhetha ulwazi malunga ne-chromatography yakho:Yonke into oyifunayo

Irabha yendalo:

Iindleko ezisebenzayo kwaye ziqhele ukusetyenziswa,I-sepler yerabha yendaloKhonza njengendlela ethembekileyo yokusetyenziswa kweenjongo ngokubanzi. Ukusetyenziswa kwabo ngokubanzi bubungqina bokusebenza kwabo kunye nokufikeleleka kwabo.

Iv. Izicelo ezi-4 zeSeptum

Uhlalutyo lokusingqongileyo:

Uhlalutyo lokusingqongileyo, ii-seplems zidlala indima ebalulekileyo ekuqinisekiseni ukuchaneka kweziphumo xa kuhlalutya umoya, amanzi kunye neesampulu zomhlaba. Umsebenzi wokutywina kubalulekile ukugcina ingqibelelo yesampulu.

Uvavanyo lwamachiza:

Ishishini lamayeza lixhomekeke kwi-sepgus ukugcina ingqibelelo yesampulu kumanqanaba ohlukeneyo ophuculo lweziyobisi kunye nenkqubo yokulawula umgangatho. Iziphumo ezichanekileyo nezinokuthenjwa zibalulekile ukuze kuqinisekiswe ukhuseleko kunye nokusebenza kweemveliso zamachiza.

Ukutya kunye nohlalutyo lwesiselo:

I-Sepguls inegalelo elikhulu ekutyeni nasekucingeni ukuvavanywa kwesiselo ngokuthintela ungcoliseko. Oku kubalulekile ukuqinisekisa ukuba umgangatho kunye nokukhuseleka kwezinto ezinokusetyenziswa.

UPhando noPhuhliso:

Elabhoratri, ii-seings Fumana izicelo zoluhlu olubanzi lweesampulu. Ukusetyenziswa kwabo kuyanceda ekufezekiseni iziphumo ezichanekileyo nezibuyisiweyo kunye nokuxhasa ukuqhubela phambili kwimimandla eyahlukeneyo yesayensi.

Ukukhetha iI-Septum yasekunene ye-chial vialEyona nto ibaluleke kakhulu ukuze kufezekiswe iziphumo ezichanekileyo nezinokuthenjwa. Esi sikhokelo sibanzi sinika abaphandi kunye nabahlalutyi kunye nolwazi abafuna ukuhambela phambili kwi-Service Forccapes ye-chromatographles, enza ukuba kuthathwe izigqibo ezizizo ezilungiselelwe uhlaziyo lweemfuno zabo zohlalutyo. Ngale ndlela, abasebenzisi banokuqalisa ukuzama kwabo ngokunyaniseka ngokuzithemba, besazi ukuba izinto zabo zohlalutyo ziya kuphumelela.

Ngaba unomdla wokufunda ngakumbi nge-HPPC ye-vial vial sepga? Vela kwinqaku lethu elipheleleyo lokuqonda kunye nesikhokelo sokukhetha i-septum elungileyo yezicelo zakho ze-HPLC: Yintoni i-vial vial sepga?

Ngaphambili:

Ndikhetha njani ubude obufaneleyo? Iinkcukacha ezi-8

Okulandelayo:

Iimpazamo ezi-5 eziqhelekileyo zokuthintela xa usebenzisa i-vialsgraphy ye-vials