I-chialsgraphy ye-chromatographyIsixhobo esibalulekileyo kwiLebhu yesayensi, siququzelela uhlalutyo oluchanekileyo lweesampulu zendlela ye-chromatographic. Ngaphandle kokusetyenziswa kwabo okubonakala ngathi kulula, iimpazamo eziqhelekileyo zinokuthoba ukuchaneka nokunyaniseka kweziphumo zovavanyo. Eli nqaku liza kuvalwa kwiimpazamo ezintlanu eziqhelekileyo ezinxulunyaniswa nee-vialsgraphy kunye neenkcukacha zokuthintela kwaye ziqinisekise ukusebenza kakuhle kwemisebenzi yakho yohlalutyo.

Ukuvula iinkqubo ezifanelekileyo zokucoca:

Ukucoca ngokucokisekileyo i-vialsgraphy ye-vials ngaphambi kokusetyenziswa linyathelo elibalulekileyo elihlala lijongelwa. Intshabalalo entsalelayo evela kwiisampulu zangaphambili inokwazisa ukungcola, okhokelela kwiziphumo ezingachanekanga kunye nongcoliseko olunqamlezileyo. Ukuthintela lo mthetho, cwangcisa iprotouthu ecocekileyo eyomeleleyo usebenzisa izinyithi ezifanelekileyo kunye neendlela ezomisayo. Rhoqo Vavanya i-vials zococeko kwaye ubeke phambili ukucoceka okungahambi kakuhle njengenkqubo esisiseko kwiindawo zelebhu.

2.Umlondolozi ohambelana

I-vialsgraphy vials iza ngeendlela ezahlukeneyo, iintlobo zokutywina kunye nee-liners, nganye yenzelwe isicelo esithile. Imicimbi egqithisileyo inokulalanisa ngentembelo yovavanyo. Ngaphambi kokukhetha i-vial, cinga ngemibandela enyange ngononophelo njengohlobo lwesampulu, indlela yohlalutyo kunye nokusebenzisana nezixhobo ze-vial. Nxibelelana nomphumo woMvelisi wokuqinisekisa ukuba i-vials ezikhethiweyo ihlangabezana neemfuno zendlela ye-chromatographic kwaye ikhusele iingxaki ezinokubakho ngexesha lohlalutyo.

Ngaba unomdla kulwazi olubanzi malunga ne-HLLC Vials? Thatha i-dive enzulu kweli nqaku ukuze umntu ahlonele yonke into ekufuneka uyazi:I-Encyclopedia ye-vials ye-HPLC

3.misebenzi yesampulu yesampulu:

Ukulungiselela kwesampulu yesampulu yesampulu yesona sizathu sokongeza iimpazamo kwiimvavanyo zeChromatographic. Ukusilela kwi-homogenise ngokufanelekileyo, icebo lokucoca okanye ukugxininisa iisampulu kunokukhokelela kwiziphumo ezingahambelaniyo nezingenakuthembeka. Inkqubo yokulungiselela imigangatho emiselweyo kufuneka iphuhliswe kwaye ilandelwe, igxininise ukubaluleka komgangatho wesampulu ngaphambi kokudluliselwa kwiI-chialsgraphy ye-chromatography. Isampulu elungiselelwe kakuhle ibalulekile kwiziphumo ezichanekileyo ze-chromatographic ezichanekileyo.

4.Umongamele i-cap:

Kubalulekile ukuba i-vial caps ivaliwe ngokuqinileyo ukuthintela i-Eveporation kunye nongcoliseko lwesampulu. Nangona kunjalo, ukuqina okungaphezulu kunokubangela iingxaki ezisebenzayo, ezinje ngobunzima bokuvula umonakalo we-vial kunye ne-septum. Ukuqinisekisa ukulinganisa okufanelekileyo, landela iingcebiso zomenzi weTorque okanye itywina xa uvala i-vialsgraphy ye-chiatography. Oku kuyakuqinisekisa itywina elikhuselekileyo ngelixa kugcinwa lula ukusetyenziswa kunye nokunyaniseka kwe-vial kuyo yonke inkqubo yohlalutyo.

Ndinomdla wokufumana ulwazi olupheleleyo malunga ne-ptfe \ / isiliki septa? Vinta eli nqaku ngokuqonda ngokupheleleyo kunye nokuqonda kwihlabathi le-Septa:Ipremiyamu ptfe kunye ne-silicone Septa: Iziko elinokuthenjwa

Iimeko zokugcina iimeko:

I-vialsgraphy yeChromatography ihlala isetyenziselwa ukugcina iisampulu ezibuthathaka, ke iimeko ezifanelekileyo zokugcina zibalulekile. Izinto ezifana nokuvezwa kokukhanya, utshintsho lobushushu lobushushu kunye nokugcina ixesha elide kunokuthoba ingqibelelo ngesampula ngexesha ukuba ungakhathalelwanga. Landela izikhokelo zokugcina ezicetyiswayo ezibonelelwe ngumvelisi we-vial ukukhusela umgangatho kunye nokuzinza kwesampulu kwixesha lonke lokugcina. Ukuqwalaselwa kweemeko zokugcina kubalulekile ukugcina ukuthembeka kwedatha yohlalutyo efunyenwe kwiimvavanyo zeChromatographic.

Ukuphepha iimpazamo eziqhelekileyo xa usebenzisaI-chialsgraphy ye-chromatographyIbaluleke kakhulu kwiimvavanyo eziphumeleleyo zohlalutyo. Ngokuthatha iindlela zokwenza izinto ezicwangcisiweyo, ukuqinisekisa ukuhambelana kwesampulu, ukwenza iinkqubo ezifanelekileyo zokucetyiswa, ukuqaphela iimeko ezifanelekileyo zokugcina, abaphandi banokunyusa ubuchwephesha be-chromatographic. Soloko ubhekisa kwizikhokelo zabavelisi kunye nezona ndlela zilungileyo zokwenza usetyenziso lwe-vials ye-chromatographic, ekugqibeleni ikhokelela kwimiphumo ephumeleleyo kwizifundo zelebhu.

Ndinomdla malunga ne-vials ye-HPLC? Dive kweli nqaku ngeempendulo ezingama-50 ezomeleleyo kunye nokomeleza ulwazi lwakho kwi-vial-chiepy ye-chromatographys:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Isikhokelo esipheleleyo kwi-septa ye-vialsgraphy ye-chromatography: iintlobo, izixhobo kunye nokusetyenziswa

Okulandelayo:

Ndikhetha njani ubungakanani be-hplc efanelekileyo?