Ama-chromatography vialsiyithuluzi elibalulekile lokugcina nokuhlaziya amasampula elabhorethri lokuhlaziya. Kodwa-ke, enye yezinkinga ezijwayelekile abacwaningi abacwaningi abavame ukuhlangana namaviki abangelwa yindaba yokubandakanya okukhona kwisampula. Lezi zinhlayiya zingaqhamuka emithonjeni ehlukahlukene, kufaka phakathi inqubo yesampula yokulungiselela, ukungcoliswa kwemvelo kanye nezihambisi uqobo. Ukuqonda izimbangela nezixazululo zalawa mavikiso kubalulekile ukuze ulondoloze ubuqotho bokuhlaziywa kwe-chromatomagraph.

Udaba oluhlanganisiwe kusampula lungenza ama-blockages ku-chromatographic vial, engathinta ukunemba nokuvela kabusha kwemiphumela yokuhlaziywa. Lezi zinhlayiya zingaphazamisa umjovo wesampula, zibangele ukuguquguquka kwengcindezi yengcindezi ye-Chromatographic futhi kungalimaza ngisho nezinto ezibucayi eziyithuluzi. Ngakho-ke kubalulekile ukubhekana nale nkinga esebenzayo.

Kunamasu ayi-7 okuvimbela futhi anciphise ama-blockages adalwe yindaba yokufaka phakathi kwi-Chromatography Vaials.

I-Sampula Filtration:

I-Sampula Filtration yisinyathelo esibalulekile ekuvikeleni ama-blockages kuma-chromatography vials. Ngaphambi komjovo ohlelweni lwe-Chromatographic, isampula idluliswa ngesihlungi ukuze ikhiphe udaba lokushaya. Izihlungi zesirinji zisetshenziselwa le njongo futhi zitholakala ezinhlotsheni ezahlukahlukene zePores ukuze zamukele izinhlobo ezahlukene zesampula. Izihlungi zeMembrane ezinjengolwelwesi lwe-nylon noma ze-PTFE nazo zisebenza nezidingo ze-FILTRATION ze-FICER. Ukukhetha usayizi ofanele wokuhlunga we-pore uqinisekisa ukuthi izinhlayiya kuphela ezingenhla komkhawulo osayizi othile zigcinwa, zinciphisa ubungozi bokuqhekeka kwe-vial.

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Ukuphathwa kwesampula efanele:

Ukuphathwa kwesampula efanele kubalulekile ukuze unciphise ukwethulwa kodaba lwama-chromatographic vial. Lokhu kufaka ukugwema ukunyakazisa okwedlulele noma ukuphazamiseka ngesikhathi sokulungiswa kwesampula, njengoba ukuxuba okunamandla kungadala izinhlayiya ukuthi zikhishwe esitsheni noma kusampula Matrix. Ukusetshenziswa kwamathuluzi ahlanzekile, i-inert anjenge-pipete yengilazi nama-vials kuzosiza ukugcina ubumsulwa besampula futhi kunciphise amathuba okungcoliswa. Ngaphezu kwalokho, ukugcina amasampula endaweni elawulwayo engenalo uthuli nakwezinye izinhlayiya zomoya kunciphisa ubungozi bokuvinjelwa.

Ukuhlolwa kwe-vial:

Ngaphambi kokusebenzisaAma-chromatography vials, ukuhlolwa okubukwayo okubukwayo kubalulekile ukuthola izimpawu ezibonakalayo zokungcola noma ukungcola. Hlola i-vial ngaphansi kwezimo ezifanele zokukhanyisa ukukhomba izinhlayiya, ukulayishwa noma okunye ukungahambi kahle. Ama-vials akhombisa izimpawu zokungcola kufanele alahlwe noma ahlanzwa kahle ngaphambi kokusetshenziswa. Ama-vials asobala asiza ekuhlolweni futhi aqinisekise ukuthi ama-vials ahlanzekile ahlanzekile kuphela asetshenziselwa ukugcinwa kwesampula kanye nokuhlaziywa.

Ukusetshenziswa kwama-vials asezingeni eliphakeme:

Ukunciphisa izinkinga ezivalekile, kubaluleke kakhulu ukukhetha ama-chromatography asezingeni eliphakeme. Ama-vials enziwe ngezinto ze-Premium ezinjenge-borossilte ingilazi anikeze ukumelana kahle kwamakhemikhali kanye nokukhishwa okuphansi kwezinhlayiyana, ukunciphisa amathuba okungcola noma ukufakwa kwendaba. Ngenye indlela, ama-polymer vials enziwe ngezinto ezifana ne-polypropylene noma i-PTFE efanelekile ukuthola izicelo ezithile ezidinga i-inert kanye neziqukathi ezingasebenzi. Ukukhetha ama-vial aqinisekisiwe ahambisana nendlela yokuhlaziya kuqinisekisa ukusebenza okuthembekile kanye nobuqotho besampula.

Ukulungiswa kwesistimu ejwayelekile:

Ukuvimbela ukuvalwa futhi kuqinisekiswe ukusebenza okuhle, kubalulekile ukusebenzisa uhlelo oluphelele lwesondlo lohlelo lwakho lwe-Chromatography. Ukuhlanza njalo kanye nokugcwala kwama-vials, ukufakwa kanye ne-tubing kususa izinhlayiya eziqoqiwe nezinsalela ezingadala ama-blockages. Sebenzisa ama-ejenti wokuhlanza okuhambisanayo nezinqubo ezinconywe ngumenzi wemishini ukugwema ukulimala kanye nokungcoliswa. Ukuhlolwa kwesistimu ejwayelekile, njengokuhlolwa kwengcindezelo nokutholwa okuvuzayo, kuzothuthukisa ukuthembeka kohlelo kanye nempilo yenkonzo.

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Ukuhlaziywa kosayizi wezinhlayiyana:

Ukwenza uhlaziye usayizi wezinhlayiyana wesampula kunikeza ukuqonda okubalulekile emvelweni nokusatshalaliswa kwendaba yokubandakanya. Amasu anjengokwehluka kwe-laser kanye ne-microscopy angasetshenziswa ukucacisa ukusatshalaliswa kosayizi wezinhlayiyana futhi akhombe osayizi abakhulu bezinhlayiyana nemithombo yokungcoliswa. Lolu lwazi lusiza ekukhetheni kwendlela efanele ye-FILTRATION kanye nezinto ezibonakalayo ze-vial zezidingo ezithile zesampula, ukunciphisa ngempumelelo ingozi yama-blockages ngesikhathi sokuhlaziywa.

Ukubambisana nabaphakeli:

Ukusebenza nabahlinzeki be-vial ne-Filtration kusiza ukufinyelela kubuchwepheshe kanye nezinsizakusebenza ukubhekana nezinkinga zokubopha. Abahlinzeki bangancoma ukucushwa okufanelekile kwe-vial, izinhlobo zokuhlunga kanye nezinqubo zokulungiswa ezisuselwa kwizidingo zakho zokuhlaziya zelebhu kanye nezimpawu zesampula. Bangase futhi banikeze ukuqeqeshwa ekuphatheni kahle nasekugcinweni ukuze basebenzise ukusebenza kwe-vial futhi banciphise ukuphazamiseka okuhlobene nokuvinjelwa. Ukusungula ubudlelwano bokubambisana kuqinisekisa ukwesekwa okuqhubekayo nokufinyelela ezixazululweni ezintsha zokugcina ubuqotho be-Chromatographic.

Ngokusebenzisa lawa masu, ilabhoratri lingabhekana ngempumelelo ama-blockages adalwe yinto eyi-inzalo ngaphakathiAma-chromatographic vialsfuthi uqinisekise imiphumela ethembekile futhi engaguquki yokuhlaziya. Ngaphezu kokuphathwa kwesampula efanelekile kanye nokulungiswa kwamathuluzi, izindlela ezisebenzayo ziyisihluthulelo sokwenza kahle ukusebenza kwe-chromatographic nekhwalithi yedatha.

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Okwedlule:

Izinkinga zokuhambisana kwezinto zokwenziwa kwe-chromatography

Olandelayo:

Ukubhekana nomonakalo wesirinji ngesikhathi sokusebenzisa