Izihlungi zesirinjiIbamba iqhaza elibalulekile emvelweni yelebhu, okwenza abacwaningi nochwepheshe bahlunga ngempumelelo amasampula ezinhlosweni ezahlukahlukene zokuhlaziya nezezilingo. Naphezu kokusebenziseka kwabo, noma kunjalo, izihlungi zesirinji zitholakala ekulimaleni ngesikhathi sokusetshenziswa, ezingadala izinkinga futhi zithinte ikhwalithi yenqubo yokuhlunga. Le ndatshana idlulela kwizimbangela ezivamile zokulimala kwehluzo lesirinji futhi inikezela ngezixazululo ezinemininingwane yokubhekana nokuvimbela lezi zinkinga.

Izimbangela zomonakalo wokuhlunga kwesirinji

Ingcindezi eyeqile:

Ingcindezi eyeqile ngesikhathiisihlungi sesirinjiUkusetshenziswa kuyimbangela eyinhloko yomonakalo wokuhlunga. Lapho usebenza ngamasampula abonakalayo noma agxiliswe kakhulu, abacwaningi bavame ukufaka amandla amaningi ngesikhathi sokuhlunga, okungaphumela ekuphumeni noma okudabula ulwelwesi lokuhlunga. Lokhu kulimazeka akugcini nje ngokubeka phansi ukusebenza kahle kwe-fieltration, kepha futhi kuholela ekulahlekelweni kwesampula okungaba khona kanye nezinkinga zokungcola.

Amakhemikhali angangcoliseki

Okunye okubalulekile okufaka isandla kumonakalo wokuhlunga kwesirinji ukusetshenziswa kwamakhemikhali angahambelani kanye nama-solvents. Izinto ezithile zingalimaza izinto ezisetshenziswe ngaphakathiIzihlungi zesirinji, wabangela ukuba babize futhi balahlekelwe yicala lokuhleleka ngokuhamba kwesikhathi. Kubalulekile ukubhekisa eshadini lokuhambisana elinikezwe ngumenzi wesihlungi bese ukhetha isihlungi esikwazi ukumelana namakhemikhali athile kwisampula.

Ukuphatha okungafanele

Ukuphathwa kabi kokuhlunga kwesirinji kungadala ukulimala ngokomzimba ezindlini zokuhlunga noma ulwelwesi. Ukuphonsa isihlungi, kunciphise kanzima, noma ukunciphisa okunamathiselwe kwalo kwisirinji kungakubangela ukuqhekeka, ukuklebhula, noma ukuhlanekezela, ukwehla inqubo yokuhlunga. Ukunakekelwa okufanele nokusingathwa ngokucophelela kubalulekile ukuvikela umonakalo onjalo.

Ukufakwa okungalungile:

Ukufakwa okungafanele kokuhlunga kwesirinji ngesirinji kungaholela ekuvuzweni noma ekulimaleni ngesikhathi sokuhlunga. Usebenzisa isihlungi esingahambelani ngokuqinile kwisirinji noma ukwehluleka ukuhlinzeka ngophawu oluqinile kungaholela ekulahlekelweni kwesampula, ukungcoliswa kwenqubo yokuhlunga. Ukugwema izinkinga ezinjalo, kubalulekile ukufaka kahle isihlungi ngokuya ngemiyalo yomenzi.

Ukuthola ulwazi oluphelele mayelana nokuhlunga okungu-0.45 micron, bhekisa kule ndaba efundwayo. Hlola imininingwane ebalulekile kanye nemikhuba engcono kakhulu yezinqubo ezisebenzayo zokuhlunga: Igayidi ephelele yokuhlunga okungu-0.45 micron: konke okudingeka ukwazi

Izisombululo ukuvikela ukulimala kokuhlunga ngezimpawu

Sebenzisa ingcindezi efanelekile: Sebenzisa ingcindezi efanele

Ukuvimbela ukulimala kokuhlunga ngenxa yengcindezi eyeqile, abacwaningi kufanele babambelele emikhawulweni ephezulu yengcindezi enconywe ngumenzi wesihlungi. Lapho uphatha amasampula anzima, njengoketshezi olunobuhlakani noma izixazululo ezihlanganisiwe, cabanga ngokuhlunga kwangaphambili noma ukunciphisa isampula ukunciphisa ingcindezi kusihlungi sesirinji.

Khetha Izihlungi ezihambisanayo:

Kubaluleke kakhulu ukukhetha isihlungi esirinji esenziwe ngento ehambelana namakhemikhali nama-solvents akhona kusampula. Bheka ishadi lokuhambisana elinikezwe ngumenzi wesihlungi ukuze uqinisekise ukuthi isihlungi osikhethayo singamelana nento ethize ehlungiwe ngaphandle kokuhlungwa ngaphandle kokuhlungwa ngaphandle kokuyekethisa ubuqotho bayo.

Vula iqiniso ngokusebenzisa izihlungi zesirinji kule ndatshana. Thola ukuthi izihlungi zesirinji zingasetshenziswa kabusha ngokuphephile futhi zenze izinqumo ezinolwazi ngezinqubo zakho zokuhlunga:Ukuhlunga ngezimpawu uzokusebenzisa futhi?

Ukuphatha Ukuqapha:

Ukuphathwa ngobumnene kubalulekile ukugwema ukulimala ngokomzimba kuisihlungi sesirinji. Ukuvimbela ukulimala ngephutha, gcina isihlungi ecaleni laso lokuvikela lapho ungasebenzi. Lapho unamathisela isihlungi kusi-syringe, faka ingcindezi emnene futhi ugweme ingcindezi noma ukucindezela okungaholela ekuqhekekeni noma izinyembezi ku-membrane yokuhlunga.

Qinisekisa ukuthi ifakwe kahle:

Ukunamathiselwa okufanele kwesihlungi se-syringe kwisirinji kubalulekile ukuvikela ukuvuza noma ukulimala ngesikhathi sokuhlunga. Qinisekisa ukuthi isihlungi sinamathele ngokuphephile kwisirinji nokuthi kugcinwa uphawu oluqinile kuyo yonke inqubo yokuhlunga. Landela imihlahlandlela yomenzi yokufakwa kwehlunga okufanele ukuze uthuthukise ukusebenza futhi uvikele ukulimala.

Hlola izimpendulo zemibuzo evamile engama-50 mayelana nokuhlunga kwesirinji kule ndaba efundwayo. Thola imininingwane ebalulekile esihlokweni sokuhlunga kwesirinji futhi uthuthukise ukuqonda kwakho:Isihloko se- "Syringe Filter" Imibuzo Ebuzwa Njalo Kaningi

Uma kwenzeka umonakalo wokuhlunga kwesirinji

Misa ukugcotshwa ngokushesha

Uma ukulimala kokuhlunga kutholwa ngesikhathi sokuhlunga, yeka inqubo ngokushesha ukuvikela ukungcoliswa okwengeziwe noma ukulahleka kwesampula. Ukugcwaliswa okuqhubekayo ngokusebenzisa isihlungi esilimele kungahlehlisa ikhwalithi nokunemba kwemiphumela.

Faka esikhundleni isihlungi

Izihlungi ezilimele kufanele zilahlwe kahle futhi zithathelwe indawo ngokuhlunga okusha, okuqondile ngaphambi kokuqala kabusha kwe-filtration. Ukusetshenziswa kwesihlungi esilimele kungahle kube nomphumela wemiphumela enganembile nezinkinga zokungcola.

Thola ukukhetha okuhle phakathi kokuhlunga kwesirinji kwe-PVDF kanye ne-nylon ngalesi sihloko esifundisayo. Thola imininingwane yokwenza izinqumo ezinolwazi zezidingo zakho zokuhlunga:Izihlungi zesirinji ze-PVDF vs.

Phenya imbangela

Thatha isikhathi sokuphenya imbangela yomonakalo wokuhlunga ukuvikela ukuphindeka. Buyekeza izimo ze-Filtration, izici zesampula, izinqubo zokuphatha, nezinye izinto okungenzeka zibe nomthelela kulo monakalo.

Idokhumenti futhi ufunde

Bhala phansi isibonelo ngasinye somonakalo wokuhlunga ngokuningiliziwe, kufaka phakathi izimo, izici zesampula, kanye nezinyathelo zokulungisa ezithathiwe. Sebenzisa lezi zigameko njengamathuba okufunda ukuthuthukisa imikhuba yokuhlunga, ukukhulisa ukusebenza kwelabhorethri, futhi uvikele ukulimala kwesihlungi esizayo.

Isihlungi sesirinjiUkulimazeka kungaphazamisa ukuhamba komsebenzi kwelabhoratri, ukuyekethisa imiphumela yokuhlunga, futhi kubangele izinkinga zokungcola zesampula. Ngokuqonda izimbangela ezivamile zomonakalo wokuhlunga kanye nokusebenzisa izindlela zokuvimbela ezifana nokusebenzisa ingcindezi efanelekile, ukukhetha izihlungi ezihambisanayo, ukuphathwa ngokucophelela, abacwaningi banganciphisa ubungozi bomonakalo wokuhlunga kanye nokugcina ubuqotho benqubo yokuhlunga. Isenzo esisheshayo uma kwenzeka kulimaze ukuhlunga, ukushintshwa kwezihlungi ezilimele, nokufunda kwalokhu okuhlangenwe nakho kuzothuthukisa ukulawulwa kwekhwalithi yelabhoratri futhi kuqinisekiswe imiphumela yokuhlaziya ethembekile.

Ukuqonda okuningiliziwe kokuhlunga okungu-0.22 micron, dlulise kule ndaba efundisayo. Vula imininingwane ebalulekile nemikhuba emihle kakhulu yezinqubo zokuhlunga:Igayidi ephelele yokuhlunga okungu-0.22 micron: konke okudingeka ukwazi

Okwedlule:

Ukubhekana nama-blockages adalwe yindaba yokufaka phakathi kwama-chromatography vials

Olandelayo:

Ukusebenza kokumelana nokuguga kweSepta ku-Chromatography Vial Caps