I-Filtration idlala indima ebaluleke kakhulu ezinkundleni ezahlukahlukene zesayensi nezimboni, isebenza njengenqubo eyisisekelo yokuhlanza amasampula ngokususa ukungcola kanye nezinhlayiya. Ukuhlunga okuphezulu kwesirinji kuphawuleka ikakhulukazi kulokhu, kunikeza ukusebenza kahle nokuguquguquka okuphezulu kuqhathaniswa nokuhlunga okujwayelekile. Noma ngabe usebenza ngelebhu, amakhemisi, ukuhlolwa kwezemvelo, noma iyiphi enye insimu edinga ukulungiswa kwe-Firration, nokwenza kahle inqubo yakho ye-fieltration ibalulekile ekufezeni imiphumela enembile nethembekile. Lapha, sidlulisela kwimihlahlandlela ephelele yokuthi ungawengeza kanjani i-samps filltration usebenzisa ukusebenza okuphezuluIzihlungi zesirinji, ukumboza konke kusuka ekuqondeni ama-membranes wokuqonda kuya ezinkingeni ezijwayelekile.

Mayelana nokuhlunga okuphezulu kwesirinji okuphezulu

Ukuhlunga okuphezulu kwesirinji kuklanyelwe kusetshenziswa izinto ezithuthukisiwe ze-membrane ezifana ne-polytetrafluoroethylene (PTFE), i-polyethersulfone (PES),injobe, ne-cellulose esetshenzisiwe. Lezi zikhumbuzo zikhethwa ekuvumelaniseni kwazo okuhle kwamakhemikhali, ukuqina okushisayo, kanye namakhono okugcina izinhlayiya, okwenza balungele izinhlelo ezahlukahlukene.

Ukukhetha ulwelwesi lokuhlunga okufanele

Ukuvumelana kwamakhemikhali:Ama-membranes wokuhlunga kufanele ahambisane nama-solvents namakhemikhali akhona kwisampula ukugwema ukonakala kwe-membrane kanye nokusebenzisana kwamakhemikhali. Ulwelwesi lwe-PTFE lwaziwa ngamandla abo okuqina kanye nokuqina kwamakhemikhali anolaka, kubenza babe ukukhetha okuthandwayo.

Ukugcinwa kwezinhlayiya:Khetha ulwelwesi ngosayizi ofanele we-pore ozogcina izinhlayiya zosayizi oyifunayo. Ukuhlunga okuphezulu kwesirinji kunikeza izinketho eziqondile zosayizi we-pore, ngokuvamile kusuka ku-0.1 kuya ku-1.0 microns noma ezincane, kuya ngezidingo zohlelo lokusebenza.

Uhlobo lwesampula:Cabanga ngohlobo lwesampula (noma ngabe namanzi, organic, noma ingxube) lapho ukhetha izinto zokuhlunga ze-membrane. Ngokwesibonelo,Ulwelwesi lwe-peszilungele amasampula anamanzi, kuyilaphoMembranes we-ptfezihle kakhulu ngokuhlunga izixazululo ze-organic ngenxa yezakhiwo zazo ze-hydrophobic.

Ufuna ukwazi izihlungi ze-micron ezi-0,22? Hlola ukuqonda okuningiliziwe kule ndaba efundwayo!:Igayidi ephelele yokuhlunga okungu-0.22 micron: konke okudingeka ukwazi

Ukwenza kahle amapharamitha we-fieltration

Ukulungiswa kwesampula:Qinisekisa ukuthi isampula ilungiswe kahle futhi ingenazinhlayiya ezinkulu noma imfucumfucu engavala isihlungi futhi ivimbele ukugeleza. Amasampula anamanani amakhulu odaba lwamaqembu okubandakanya angadinga ukugcwala kwangaphambi kokuhlunga noma nge-centrifugation.

Uhlobo lwesirinji nosayizi:Sebenzisa isirinji esezingeni eliphakeme nge-LEER-Lock Fitting ukuqinisekisa ukuxhumana okuphephile, okuvuzayo okulula kusihlungi. Usayizi wesirinji kufanele kufanelekile ngevolumu yesampula ukuthi ihlungwe, igweme ukulayisha ngokweqile noma ukungasebenzi kahle kwesihlungi.

Ijubane lokuhlunga:Ibhalansi phakathi kwejubane lokuhlunga kanye nokusebenza kahle kubalulekile. Ngenkathi izinga lokuhlunga eliphakeme lifiseleka ngokukhiqiza, isilinganiso esiphakeme kakhulu sokugeleza singalimaza ulwelwesi futhi sinciphise ukusebenza kahle kokugcina. Landela izincomo zomkhiqizi ze-Flow rate efanelekile esekelwe ku-membrane nezimpawu zesampula.

Ukuhlanza umoya: Ngaphambi kokuhlunga, susa ama-bubble omoya abhajwe emhlanganweni wesirinji kanye nesihlungi ukuvikela izingidi zomoya futhi uqinisekise ukugeleza okungaguquki kuyo yonke inqubo yokuhlunga.

Gcina ubuqotho bokuhlunga

Ukufakwa esikhundleni sesikhathi:Ukuvimbela ukuvalwa futhi uqinisekise ukusebenza okuthembekile kwe-filltration, buyisela izihlungi ngemuva kokusetshenziswa noma ngokuya ngemihlahlandlela yomenzi. Ukusetshenziswa isikhathi eside kokuhlunga okungaphezulu kwempilo yabo enconyiwe kungahlehlisa ubuqotho bokuhlunga kanye nokusebenza kahle kwe-filltration.

Izimo Zokugcina:Ukugwema ukungcoliswa nokugcina ubuqotho be-membrane, gcina izihlungi ezingasetshenziswanga endaweni ehlanzekile, eyomile endaweni yokushisa enconyiwe. Isitoreji esingafanele singaholela ekunciphiseni kwezinto zokufumba nge-membrane futhi sithinte ikhwalithi yokuhlunga.

Ukubuyela emuva:Ezimweni eziyinselelo ezinselele ikakhulukazi noma lapho inhloso ukunweba impilo yokuhlunga, cabanga ukubuyisela emuva isihlungi nge-solvent ehambisanayo yokususa izinhlayiya eziboshwe kanye nokugcina.

Unentshisekelo yokufunda ngezihlungi ze-micro? Ngena kule ndatshana ephelele imininingwane enemininingwane!:Igayidi ephelele yokuhlunga okungu-0.45 micron: konke okudingeka ukwazi

Ukuxazulula inkinga izinkinga ezijwayelekile

I-Filtration ehamba kancane:Uma i-filltration ihamba kancane kunalokho obekulindelwe, hlola ukuvalwa kwe-membrane, ukulungiswa kwesampula okungafanele, noma amapharamitha wokuhlunga angalungile. Lungisa amapharamitha njengoba kunesidingo noma ushintshe usayizi omkhulu we-pore ukuze uqinisekise ukugeleza okusebenzayo ngaphandle kokuvuselelwa kwenhlayiya.

Izinkinga zokuvuza noma zokubopha uphawu:Hlolaisihlungi sesirinji, futhi uLuer Ukhiye ofanele umhlangano wokuqondanisa kahle nokuqina. Vikela kahle konke ukuxhumana ukuvikela ukuvuza okungahlehlisa ukunemba kwe-filltration kanye nokuchitha isampula ebalulekile.

Ukulimala kukaMembrane:Gwema ukusebenzisa izixazululo noma izingcindezi ezingenhla ezinconyiwe ezingalimaza ulwelwesi lokuhlunga. Khetha impahla ye-membrane efanelekile kumakhemikhali akho wesampula ukuqinisekisa impilo ende nokusebenza okungaguquki.

Ukukhethwa ngokucophelela kokuhlunga okuphezulu kwesirinji okuphezulu, ukusebenza kahle kwamapharamitha okuhlunga, kanye nokunamathela ekunakekelweni kahle nasekuxazululeni izinkinga kungathuthukisa ukusebenza kahle, ukuthembeka, kanye nokude kwenqubo yokuhlunga yesampula. Le mizamo ifaka umthelela emiphumeleni enembile futhi engaguquki futhi izuze izinhlelo ezahlukahlukene zesayensi, ezenziwe ngemithi kanye nezimboni lapho ukugcwala okunembile kubaluleke kakhulu.

Okwedlule:

Ukuthuthukisa Ukusobala Namazinga Wokulebula We-Chromatogragraphy Vavials Ekuhlolweni Kwezemvelo

Olandelayo:

Ungayikhetha kanjani uhlobo lwe-PTFE lwe-PTFE lwe-Septa for Chromatography Vaials yakho