I-Chromatography iyindlela esetshenziswa kabanzi elabhorethri ukuze ihlukanise futhi ihlaziye amakhompiyutha. Enye yezinto ezisemqoka ze-chromatography vial yi-septum. I-Septum isithiyo esifaka uphawu lwe-vial futhi ivumela isampula ukuthi lifakwe ku-chromatograph. Wezinto ezahlukahlukene ze-septum ezitholakalayo,I-PTFE (polytetrafluoroethylene)) i-septumzincanyelwa kakhulu ukumelana kwazo kwamakhemikhali nokungapheliyo. Kodwa-ke, ukukhetha uhlobo olufanele lwe-PTFE-cail septum kudinga ukucatshangelwa kwezici eziningana ukuqinisekisa ukusebenza kahle kanye nokuhambisana nezidingo zakho ze-Chromatographic. Amanye amaphuzu abalulekile okufanele uhlale engqondweni abhalwe ngezansi.

1. Ukuvumelana Kwamakhemikhali

Ngaphambi kokukhetha i-PTFE-eboshwe nge-septum, kubalulekile ukukhomba amakhemikhali nama-solvents abandakanyeka ekuhlaziyweni kwe-chromatomagraphic. I-PTFE yaziwa ngokumelana kwayo okuhle kwamakhemikhali, okwenza kube lula ukuthola izicelo ezahlukahlukene. Kuyamelana nama-acid, izisekelo, izixazululo ze-organic, namanye amakhemikhali amaningi, kanti amakhemikhali amaningi ahlala eqinile futhi ngeke ahlangane nesampula noma athinte kabi ukuhlaziya. Kodwa-ke, kubalulekile ukuqinisekisa ukuhambisana okuthile namakhemikhali asetshenziswa elabhorethri ukugwema izinkinga nokungcola.

2. Ukuqina kokushisa

Amasu we-Chromatographic avame ukufaka ukuvezwa kwamazinga okushisa aphezulu, ikakhulukazi kugesi i-chromatography (GC) kanye ne-chromatography ephezulu ye-chromatography (HPLC). Ikhwalithi ephezuluI-Ptfe-camera ehlanganisiwezenzelwe ukubekezelela la mazinga okushisa ngaphandle kokuwohloka, ukuqinisekisa ukusebenza okungaguquki kanye nokubekwa uphawu okuthembekile kwezipesheli. Kubalulekile ukubheka imininingwane yobubanzi bokushisa enikezwe ngumenzi ukuqinisekisa ukuthi i-septum ingaphatha ukushisa okwenziwe ngesikhathi senqubo ye-chromatomatic.

3. Ubukhulu nokuqina

Ubukhulu bokuhlanganiswa kwe-PTFE kwi-Septum kudlala indima ebalulekile ekudakeni nasekude kwe-Septum. Ukuhlanganiswa okuqinile kuthuthukisa ukumelana nokugxuma futhi kunciphise ubungozi bomonakalo kwi-Septum ngesikhathi semijovo eminingi. Lokhu kubaluleke kakhulu ezindaweni zokusebenzisa eziphakeme kakhulu lapho kusetshenziswa khona ama-vials futhi ahluthwe kaningi. I-coating emile futhi inwebisa impilo ye-septum, inciphisa isidingo sokubuyiselwa njalo kanye nokuqinisekisa ukusebenza okungaguquki ngokuhamba kwesikhathi.

Ufuna ukwandisa ulwazi lwakho ku-HPLC VIAL Septa? Ngena kule ndatshana ngokuqonda okuningiliziwe kanye nemininingwane ebalulekile ekukhetheni nge-Septe ye-Septa yakho:Iyini i-HPLC VIAL Septa?

4. Ukungena Ngenaliti Nezakhiwo Zokuzenzisa

Ukungeniswa kwenaliti esebenza kahle kubalulekile emjovo wesampula oshelelezi futhi onembile ku-chromatography. I-PTFE-COMPACETS kumele ivumele ukungena kalula ukungena kwemali ngaphandle kokukhanyisa noma ukushiya imfucumfucu engangcolisa isampula noma iphazamise ngokuhlaziywa. Ngaphezu kwalokho, ukuze ugcine ubuqotho be-vial futhi uvikele ukuhwamuka noma ukuvuvukala kwesampula, i-septum kumele ibonise izakhiwo zokuzibeka uphawu ngemuva kokuthi inaliti isusiwe. Lokhu kuqinisekisa imiphumela ethembekile nezenzekayo ngemijovo eminingi.

I-5. Ukucatshangelwa okuphathelene nohlelo lokusebenza

Kuya ngohlelo lwe-Chromatographic, kungahle kube nezidingo ezithile ezithinta ukukhetha kwePtfe-camera camera. Isibonelo, ku-chromatography yegesi (GC), lapho kuhlaziywa khona amakhompiyutha aguqukayo, kubalulekile ukukhetha i-Septum enciphisa ukulahleka kwesampula ngenxa yokuhwamuka. Ngokuphambene nalokho, ukuhlaziya okubucayi kwe-Ultra okudinga amazinga aphansi angemuva, i-septum enezinto ezimbi kakhulu futhi kunconyelwa ukugwema ukuphazamiseka ngemiphumela yesampula. Lapho ukhetha i-Septum, cabanga ngezidingo ezithile ze-Chromatographic indlela nohlobo lwesampula.

Unentshisekelo yokufunda mayelana nezinhlelo zokusebenza eziyi-15 zesimo se-Chromatography Vials? Hlola le ndatshana ngokuqonda okujulile nokubuka konke okuphelele kokusetshenziswa kwazo okuhlukahlukene:Izicelo eziyi-15 zesimo se-Chromatography emikhakheni ehlukene

6. Uhlobo lwe-vial kanye nosayizi ukuhambisana

I-PTFE-camera ehlanganisiwe iyatholakala ezinhlotsheni ezahlukahlukene kanye nemiklamo ukuze ilungeAma-chromatography vials. Ukufeza ukubekwa uphawu okufanele futhi kuvikele nezinkinga ezinjengokuvuza noma ukubekwa uphawu okungafanele, kubalulekile ukuqinisekisa ukuthi i-septum iyahambisana nohlobo lwe-vial esetshenziswayo. Hlola imininingwane enikezwe ngumenzi ukukhetha usayizi ofanele we-Seputum, isitayela (esivaliwe noma esiqinile, njll.), Nokucushwa noma okungavinjelwe, njll) ngezidingo zakho ze-vial.

7. Idumela lomkhiqizi kanye nokuqinisekiswa kwekhwalithi

Lapho ukhetha aPtfe-camera camera, cabanga ngedumela lomenzi kanye nemikhuba yokuqinisekiswa kwekhwalithi. Abakhiqizi abadumile banamathela ezindinganisweni eziqinile zekhwalithi futhi bangahlinzeka ngezitifiketi nokuvunyelwa okuqinisekisa ikhwalithi ye-septum kanye nokufaneleka kwezicelo ze-chromatomatographic. Funa imikhiqizo evela kubahlinzeki abadumile abahlangabezana namazinga embonini futhi babe nerekhodi elifakazelwe lokukhiqiza i-septam elithembekile elinomthelela ekunembeni, ukuvela kabusha kanye nokusebenza kahle kokuhlaziywa kwe-chromatographic.

Ekuphetheni, ukhetha uhlobo olufanele lwe-PTFE-camera ecated septum yesivinini se-chromatography idinga ukuhlolwa kwezici okufana nokulinganiswa kwamakhemikhali, izidingo zokuzenzakalelayo, ukuqinisekiswa kohlelo lokusebenza, kanye nokuqinisekiswa kwekhwalithi yomkhiqizi. Lokhu okulandelayo kungenye yezinto ezidinga ukuhlolwa. Lokhu okulandelayo ukubuka okufushane kwezinto okufanele kubhekwe ekuhlolweni kwe-vial ecubungula lezi zinto nokukhetha i-septum esezingeni elifanele kusuka kumkhiqizi odumile kungathuthukisa ukunemba, ukuvela kabusha kanye nokusebenza kahle kokuhlaziywa kwakho kwe-chromatographic.

Ukuqonda okuphelele kwe-PTFE \ abicone septa, ngena kule ndatshana efundisayo. Thola ukuqonda okubalulekile nobuchwepheshe ekukhetheni kwe-SEPTA!:Konke odinga ukukwazi: 137 pre-slit ptfe \ airtone septa FAQs

Okwedlule:

Ukuthuthukisa iSampula Filtration: Igayidi ebanzi yokwenza kahle ukusebenza ngezihlungi zesirinji eziphezulu

Olandelayo:

Izinkinga zekhwalithi kuma-Chromatogragraphy Vavials: Izimbangela Nemiphumela