Ukulungiswa kwesampuli kuyisinyathelo esibucayi ku-chromatography-mass spectrometry (Inqubo ye-LC-MS), ithonya ukunemba kanye nokuthembela kwemiphumela yokuhlaziya. Inhloso esemqoka yokulungiswa kwesampula ukukhipha, ukuhlanza, nokugxilisa ukuhlaziya kusuka kumatric ayinkimbinkimbi, ukuqinisekisa ukuthi ziyahambelana nokuhlaziywa kwe-LC-MS. Lesi sinyathelo sibucayi ukuqeda okungaba khona okungabamba ikhwalithi yedatha. Ukulungiswa kwesampula okufanele kuthuthukisa ukuzwela nokucaciswa kokuhlaziywa, okubalulekile ukuze kusetshenziswe kwizicelo ezinkambeni ezahlukahlukene njengama-proteomics, metabolomics, ukuhlaziya kwemithi kanye nokuhlolwa kwezemvelo.

Izinhloso ezibalulekile zokulungiswa kwesampula:

Ukususwa kokuphazamiseka: Susa izinto ezingaphazamisa ukutholwa kokuhlaziywa okuhlosiwe.

Ukuqoshwa kokuhlaziywa: Khulisa ukugcwala kokuhlaziywa kokuthola umkhawulo wokutholwa.

Ukuvumelana: Lungiselela amasampula ngendlela abangahlaziywa ngempumelelo yi-LC-MS Systems.

Ufuna ukwazi kabanzi nge-HPLC Sampula Ukulungiselela, sicela ubheke le ndatshana: I-HPLC isampula izixazululo zokulungiselela imiphumela emihle kakhulu

Amasu ajwayelekile ekulungiseleleni amasampula we-LC-MS

Amasu ambalwa asetshenziswa kakhulu kulungiselelo lweSampula ye-LC-MS. Indlela ngayinye inezinzuzo zayo futhi ikhethiwe ngokuya ngezidingo ezithile zokuhlaziywa.

1. Ukukhishwa kwesigaba esiqinile (i-SPE)

I-SPE isetshenziselwa kakhulu ukuhlaziya okuhlukanisayo kumasampula awuketshezi. Le ndlela ifaka ukudlulisa isampula ngesigaba seminyaka esiqinile egcina ngokukhetha ukuhlaziya okuhlosiwe ngenkathi ivumela ezinye izakhi ukuthi zidlule. I-SPE isebenza ngokukhethekile kumatikuletsheni eziyinkimbinkimbi njengegazi noma umchamo futhi unikeza izinzuzo eziningana:

Amanani aphezulu wokutakula: I-SPE imvamisa ithela amanani aphezulu wokutakula ngokuqhathaniswa nokukhishwa okuwuketshezi.

Ukuvumelana okuzenzakalelayo: Indlela ingashintshwa kalula, ukuthuthukisa ukukhishwa kanye nokusebenza kahle.

2. I-Protein Perigitation

I-Protein Precipitation iyindlela elula nokho ephumelelayo yokususa amaprotheni kumasampula endalo. Le ndlela ibaluleke kakhulu lapho ukuhlaziya amasampula anokuqukethwe okuphezulu kwamaprotheni, njenge-plasma noma i-serum. Ngokufaka amaprotheni, le ndlela yenza lula i-matrix futhi yehlise ukuphazanyiswa ekuhlaziyweni okulandelayo.

Ngokushesha nangokusebenza kahle: Kuvumela ukucutshungulwa ngokushesha kwamasampula ngenkathi kugcinwa amanani amahle okutakula.

3. Ukukhishwa okuwuketshezi (i-Lle)

U-Lle uhlukanisa amahlaya asuselwa kwi-solubenity yabo kuma-liquid amabili angenakuqhathaniswa - imvamisa isigaba esinamanzi kanye ne-organic solvent. Le ndlela iyasiza ekukhimaneni okungabingeli noma ngokulinganisela ama-polar ama-polar avela kumatikuletsheni amanzi anzima.

Uhlelo lokusebenza lwe-Versatile: I-Lle ingashintshwa amavolumu nezinhlobo ezahlukahlukene zesampula, okwenza kube lula ukuthola izicelo ezahlukahlukene.

4. Ukusulwa

Ukutholwa kwe-Derivatization kubandakanya ukuguqula amakhemikhali ukuguqula ukuzokwakhiwa kwabo ekuhlaziyweni kwe-LC-MS. Le nqubo ithuthukisa izakhiwo ezifana nokuguquguquka nokusebenza kahle kwe-ionization, okwenza kube wusizo ikakhulukazi kwizinkomba ezinokuthola okuphansi okukhona.

Ukuzwela okuthuthukisiwe: Ukufunwa kwemali kungakhulisa kakhulu ukuzwela nokukhetha kokuhlaziya kwamakilasi athile ahlanganisiwe afana nama-amino acid noma ama-steroid.

Ufuna ukwazi kabanzi mayelana nokusetshenziswa kwama-chromatography vials, sicela ubheke le ndatshana: Izicelo eziyi-15 zesimo se-Chromatography emikhakheni ehlukene

Izici ezithonya Ukukhetha Kwendlela Yokulungiselela Isampula

Ukukhetha inqubo efanelekile yokulungiselela isampula kudinga ukucatshangelwa kwezinto eziningana:

I-Analyte Chemistry: Ukuqonda ubumbano, ukushaja, nokuqina kokuhlaziywa kubalulekile.

Isampula yevolumu nobunzima: izingqinamba zevolumu nobunzima be-matrix yesampula kungakubeka ukuthi yiziphi izindlela ezinokwenzeka.

Izidingo zokuzwela: Izicelo ezahlukahlukene zingaba nezidingo zokuzwela ezahlukahlukene ezithonya lokukhetha indlela.

Izinsizakusebenza zelebhu: Ukutholakala kwemishini ye-automation noma ukucubungula okwenziwa ngesandla kungathinta ukukhethwa kwenqubo.

Imikhuba emihle kakhulu yokulungiselela isampula esebenzayo

Ukuthola imiphumela ephezulu ekuhlaziyweni kwe-LC-MS, ukunamathela kwimikhuba emihle kakhulu ngesikhathi sokulungiswa kwesampula kubalulekile:

Ukulinganisa: ukuthuthukisa ama-protocol ajwayelekile ukuqinisekisa ukuvumelana kokuhlaziya.

Ukuqinisekiswa: Vumelana nezindlela zokuqinisekisa ukuthembeka nokunemba kwazo.

Nciphisa ukuphatha isampula: Yehlisa inani lokukhohlisa ukuvimbela ukulahleka noma ukungcoliswa.

Sebenzisa ama-reagents wekhwalithi: sebenzisa ama-reagents asezingeni eliphakeme ukugwema ukwethula ukungcola kube amasampula.

Izinqubo zemibhalo: gcina amarekhodi anemininingwane yazo zonke izinqubo ukwenza lula ukuxazulula inkinga kanye nokwenza kahle kwendlela.

Ukugcina

Ukulungiswa kwesampula okusebenzayo kubalulekile ekuhlaziyweni kwe-LC-M-MS ephumelelayo. Ngokuqonda amasu ahlukahlukene afana ne-SPE, i-Propein Precistage, Lle, kanye nokutholwa, abahlaziyi bangakhetha indlela efanelekile yezidingo zabo ezithile. Ngaphezu kwalokho, ukulandela izindlela ezinhle kakhulu kuzothuthukisa ikhwalithi yedatha futhi kuqinisekiswe imiphumela ethembekile yezinhlelo zokusebenza ezihlukile kuzilungiselelo zezimboni.

Ekuphetheni, amasu okusebenza kahle amasampula angeke athuthukise ukusebenza kokuhlaziya, kepha futhi enza iminikelo ebalulekile yokuthuthukela ezindaweni ezinjengokwelashwa, ukuthuthukiswa kwezidakamizwa, kanye nokuqapha imvelo.

Vula izimfihlo zokulungiselela okulungile kwe-chromatography vial kokuhlaziywa okunembile nangokuthembekile ngezinyathelo ezi-6 ezilula.Funda ukuze ulisebenzise kahle inqubo! Izinyathelo ezi-6 zokulungiselela ama-chromatography vials ukuze ahlaziywe

Okwedlule:

I-LCMS vs GCMS: Yikuphi inqubo engcono kakhulu ocwaningweni lwakho?

Olandelayo:

Yini i-toc vial nokubaluleka kwayo ku-chromatography?