I-Gesi Chromatography-Mass Spectrometry (GC-MS) kanye ne-Chromatography-Mass Spectrometry (LC-MS Spectrometry (LC-MS) yizindlela ezimbili zokuhlaziya ezisetshenziswa kabanzi emikhakheni ehlukahlukene efana nesayensi yezemvelo, amakhamishini kanye nokuphepha kokudla. Zombili lezi zindlela zenzelwe ukuhlukanisa nokukhomba amakhompiyutha ekuxubeni okuyinkimbinkimbi, kepha izimiso zazo zokusebenza, izinhlelo zokusebenza, nezinzuzo zihluke kakhulu. Le ndatshana izohlola umehluko phakathi kwe-GC-MS ne-LC-MS ukusiza abacwaningi ukuthi babone ukuthi iyiphi indlela engcono kakhulu ngezidingo zabo ezithile.

Vula izimfihlo zokulungiselela okulungile kwe-chromatography vial kokuhlaziywa okunembile futhi okuthembekile ngaphakathiIzinyathelo ezi-6 ezilula nje. Funda ukuze ulisebenzise kahle inqubo!

I-GC-MS ne-LC-MS Overview

Igesi Chromatography-Mass Spectrometry (GC-MS)

I-GC-MS ihlanganisa i-chromatography yegesi kanye ne-mass spectrometry ukuhlaziya amakhompiyutha aguqukayo kanye nama-semivolatile. Kule ndlela, isampula ihanjiswa futhi ihanjiswa ngekholomu ye-chromatographic ngegesi ye-inert, imvamisa i-helium. Ingxenye yegesi ye-chromatography ihlukanisa amakhompiyutha asuselwa ekuguqukeni kwawo kanye nokuxhumana nesigaba seminyaka yekholomu. Ngemuva kokuhlukaniswa, amakhompiyutha angeniswa kwi-mass eploctrometer lapho anama-Ioned kanye nesilinganiso sabo sokukhokhisa. Le nqubo ingakhomba futhi ikhethe amakhompiyutha akhona kwisampula.

Izicelo ze-GC-MS:

Ukuhlaziywa kwe-forensic: I-GC-MS iyindinganiso yegolide yokuhlonza izidakamizwa nobuthi kumasampula endalo.

Ukuqapha imvelo: Kusetshenziselwa ukuthola ukungcola kanye nezinto eziyingozi emoyeni, emanzini, nasenhlabathini.

Ukuphepha kokudla: I-GC-MS ingakhomba izinsalela zokubulala izinambuzane kanye nokunye ukungcola emikhiqizweni yokudla.

Ukuhlaziywa kwe-petrochemical: Kuqashwa ukuhlaziya imikhiqizo kaphethiloli kanye nokutholwa kwazo.

Ufuna ukwazi kabanzi ukuthi kungani ama-headpace vials asetshenziswa ku-chromatography ?, Sicela ubheke le Artice:Kungani ama-headspace vials asetshenziswa ku-chromatography? 12 engles

I-Chromatography ewuketshezi-Mass Spectrometry (LC-MS)

I-LC-MS ihlanganisa i-chromatography ewuketshezi nge-mass spectrometry, okwenza kube lula ukuhlaziya uhla olubanzi lwezinhlanganisela, kufaka phakathi lezo ezingenakuzinzisa noma ezingaguquki. Ku-LC-MS, isampula lichithwe esigabeni se-Liquid Mobile, esidonswa ngekholomu egcwele phama ngesigaba seminyaka. Amakhompiyutha ahlukaniswe ngokususelwa kwizakhiwo zawo zamakhemikhali, nangemva kokuhlukana, angowokuhlukaniswa futhi ahlaziywa yi-Mass Spectrometer.

Izicelo ze-LC-MS:

Ucwaningo lwezemithi: I-LC-MS isetshenziselwa kakhulu ukuthuthukiswa kwezidakamizwa, kufaka phakathi amakhemisi kanye nokuhlonza imetabolite.

I-Biotechnology: Kubalulekile ukuze uhlaziye amaprotheni, ama-peptides, nama-acic acid.

Ukuxilongwa kwemitholampilo: I-LC-MS iqashwa ekuhlaziyweni kwama-biomaskers kanye nemithi yokwelapha kumasampula emitholampilo.

Ukuhlaziywa Kwezemvelo: Kuyafana ne-GC-MS, i-LC-MS isetshenziselwa ukuthola izingodo kumatikuletsheni ahlukahlukene, kufaka phakathi amanzi nenhlabathi.

Ufuna ukwazi kabanzi mayelana nokusetshenziswa kwama-chromatography vials, sicela ubheke le ndatshana: Izicelo eziyi-15 zesimo se-Chromatography emikhakheni ehlukene

Umehluko Osemqoka phakathi kwe-GC-MS ne-LC-MS

1. Isigaba seselula

Umehluko obaluleke kakhulu phakathi kwe-GC-MS ne-LC-MS yisigaba seselula esisetshenziselwa ukwahlukana. I-GC-MS isebenzisa isigaba se-ger mobile, okwenza kube yinto elungele amakhompiyutha aguqukayo nangokweguqukayo. Ngokuphambene nalokho, i-LC-MS isebenzisa isigaba se-liquid mobile, ivumela ukuhlaziywa kwezinhlanganisela eziningi ezibanzi, kufaka phakathi lezo ezingenakuvinjwa ngaphandle kokuwohloka.

2. Ukulungiswa kwesampula kanye nokuhambisana

I-GC-MS idinga amasampula ukuthi abe se-solvent engeyona eye-polar futhi kufanele ahlukaniswe nge-vaporised ngaphambi kokuhlaziywa. Lesi sidingo sikhawulela ukusebenza kwaso kumakhompiyutha anamaphoyinti aphansi aphansi kanye nokuqina okushisayo. Ngakolunye uhlangothi, ama-LC-MS angahlaziya amasampula kuma-Solrol Sols anama-polar futhi ahambelana kakhudlwana ngama-matric ayinkimbinkimbi, okwenza kufanelekile ngohlu olubanzi lokuhlaziywa, kufaka phakathi i-biomolecule enkulu.

3. Ukuzwela kanye nemikhawulo yokutholwa

Womabili la masu anikela ngokuzwela okuphezulu, kepha ukusebenza kwawo kungahluka ngokuya nge-analytes. I-GC-MS ngokuvamile izwela kakhulu amakhompiyutha aguquguqukayo, kanti ama-LC-MS anokuzwela okuphezulu kwezindawo ezingezona eziguquguqukayo futhi ezishisayo. I-LC-MS ingafinyelela futhi imikhawulo yokutholwa ephansi yamakilasi athile we-compounds, njengezemithi nama-biomolecule.

4. Izindleko zokusebenza nobunzima

Izinhlelo ze-GC-MS zivame ukuba yinkimbinkimbi futhi zidinga ukuqeqeshwa okukhethekile okukhethekile kunezinhlelo ze-LC-MS. Ngenxa yalokhu, i-GC-MS ingabiza kakhulu ama-laboratories ngezingqinamba zesabelomali. I-LC-MS, ngenkathi inikeza ukusetshenziswa okubanzi, kubandakanya izakhi eziningi nokulungiswa, okungakhuphula izindleko zokusebenza.

Ukukhetha inqubo efanelekile yocwaningo lwakho

Lapho unquma phakathi kwe-GC-MS ne-LC-MS, abacwaningi kufanele bacabangele izinto eziningana:

Uhlobo Lwamahlaziya: Uma amakhompiyutha okuhlosiwe angama-turatile futhi azinzile, i-GC-MS ingaba ukukhetha okuthandwayo. Okwezinkomba ezinkulu, ezingaguquki, noma ezibucayi ezingazinzile, i-LC-MS ifanelekile ngokwengeziwe.

Isampula Matrix: Ubulukhuni be-matrix yesampula bungathonya ukukhethwa kwenqubo. I-LC-MS ivame kangcono kumasampula e-biological, ngenkathi i-GC-MS idlula kwizicelo zezemvelo nezemvelo.

Izidingo zokuzwela

Isabelomali Nezinsizakusebenza: Cabanga ngezindleko zokusebenza, kufaka phakathi ukulungiswa nokuqeqeshwa, lapho ukhetha inqubo. I-GC-MS ingahle ibe nentukuthelo yama-lateratories amancane anezinsiza ezikhawulelwe.

Ufuna ukwazi izimpendulo ezingama-50 mayelana ne-HPLC VIALS, sicela uhlole le ndatshana: Imibuzo evame ukubuzwa kaningi ku-HPLC VIALS

Ukugcina

Kokubili i-GC-MS ne-LC-MS ngamasu anamandla wokuhlaziya anezinzuzo ezihlukile nezinhlelo zokusebenza. I-GC-MS ilungele ukuhlaziya amakhompiyutha aguqukayo futhi isetshenziswa kabanzi ekuhlaziyeni kwe-forensic kanye nemvelo. Ngokuphambene, i-LC-MS inokusebenza okubanzi kwezindawo ezingekho emthethweni kanye namakhompiyutha we-anrvile, okwenza kube yinto ebaluleke kakhulu ekucwaningweni kwemithi kanye ne-biotechnology. Ekugcineni, ukukhetha phakathi kwe-GC-MS kanye ne-LC-MS kufanele kususelwe kwizidingo ezithile zocwaningo, kufaka phakathi uhlobo lokuhlaziywa, ubunzima be-matrix yesampula, izidingo zokuzwela, nezinsizakusebenza ezitholakalayo. Ngokucabanga ngalezi zinto, abacwaningi bangakhetha inqubo efanelekile yokuthola imiphumela enembile nethembekile ekuhlaziyeni kwabo.

Okwedlule:

I-HPLC vs Electrophoresis: okufanele uyisebenzise?

Olandelayo:

Konke odinga ukukwazi nge-LCMS Sampula Ukulungiselela