Kukulungiswa kwesampula ye-HPLC, izihlungi zesirinji zidlala indima ebalulekile. Ama-Phala amasampula angakwazi ukuvala amakholomu, impilo yekholomu yokufushanisa, amasiginali we-defcise detector, kanye nokuyekethisa kabusha. I-Pre-Filtration isusa lokhu kungcola, ngcono ukuphindaphinda nokuzinza kwesisekelo. Izihlungi zesitayela sezinaliti zinikeza isixazululo esisebenziseka kalula, ukuqinisekisa ukuthi amasampula ahlanzekile ngaphambi komjovo, ukuvikela zombili ikholomu kanye nensimbi, kanye nokwenza ngcono ukuthembeka kokuhlaziya

Izici ezibalulekile zezinto zokuhlunga ezihlukile ze-membrane

Ptfe (polytetrafluoroethylene) membranes

Ukuvumelana kwamakhemikhali:Elert ngokuhlukile futhi wamelana nakho konke ukuqina kwe-organic sol, ama-acid, kanye nezisekelo, kodwa ayikufanele. Ilungele i-Solvents ephezulu yePolarity (E.G., Acetonitrile, DMSO), Izinga eliphakeme lokushisa nezindawo ezilimazayo. Ikhipha cishe awekho ama-extractables.
I-Flow Rate:Isakhiwo esiminyene sidinga ukufakwa kwangaphambili nge-organic solvent, okuholele kumanani aphansi okugeleza.
I-Protein ebophayo: I-Hydrophobic surfavil iveza ama-adsorption ama-protein ancinithekile angenamisebenzi, okwenza kube kuhle ukuhlaziya okulandela umkhondo.Sebenzisa amacala nemikhawulo:Okungcono kakhulu kumasampula anokhahlo noma anama-polar organic (acid acid, extracts reagent). Hhayi amanzi ngaphandle kokumanzisa; Gwema ukomisa uMefbrane ukuvikela izinyembezi.

Ama-nylon membranes
Ukuvumelana kwamakhemikhali:Hydrophilic; iyahambisana namanzi kanye ne-organic solvents eningi. I-SolVents ejwayelekile efana ne-acetonitritrile ne-DMSO isekelwa ngokuphelele, kuyilapho i-methanol ye-thf ne-high-gxity ingaphansi kokuqapha. Ukuqina kwehla ngama-acid aqinile \ izisekelo.I-Flow Rate: Ama-wets alinganiselayo ngokushesha futhi izihlungi ngokushesha kune-PTFE, kepha ngokuvamile kancane kune-PVDF.I-Protein ebophayo:I-adsorption ephezulu engacacisiwe-engafanele ama-protein-rich amasampula adinga ukululama okuphezulu. Sebenzisa amacala nemikhawulo: I-Versatile ye-Organic Solvent noma amasampula anamanzi amaningi (e.g., acetonitrile, Methanol). I-rugged and autoclavable, kepha gwema amaprotheni noma ama-strong-acid \ amasampula asesisekelo.

I-PVDF (Polyvinylidene Fluoride) Membranes
Ukuvumelana kwamakhemikhali:Hydrophilic; uzinze ngamanzi, ama-alcohols, nama-ketones angathathi hlangothi ku-acidic \ eyisisekelo izimo eziyisisekelo. I-UV ephansi kakhulu inciphisa ukuphazamiseka kwangemuva.I-Flow Rate: Ama-Wets aphezulu Kalula, Ukunikela nge-filtration esheshayo kune-PTFE ngosayizi ofanayo we-poreI-Protein ebophayo:I-Low-ilungele amasampula endalo eziphilayo, amaprotheni, kanye nemidiya yesiko leselula.
Sebenzisa amacala nemikhawulo:Ilungele i-protein \ i-peptide noma i-alcohol-based paramp fieltration. Uzwela izisekelo eziqinile, ezingangcolisa ulwelwesi.

I-PC (polycarbonate) membranes
Ukuvumelana kwamakhemikhali: I-Smooth, ebumbaneni ebushelelezi lihambisana nama-acid amaningi, usawoti, oyela, kanye nama-hydrocarbons agcwele. Hhayi ukumelana ne-methanol, ama-ketones, izithembiso, noma ama-aromatics.I-Flow Rate:Ukusabalalisa okufana kakhulu ne-pore okufanayo kuthela i-backssure ephansi nokugeleza okusheshayo.I-Protein ebophayo:Ingaphezulu eliphansi kakhulu lengilazi livimba ama-adsorption amaprotheni nama-molecule amakhulu.
Sebenzisa amacala nemikhawulo:Ilungele ukubalwa kwezinhlayiyana, ukuhlaziya kwezinhlayiyana okuyizinhlayiyana, kanye nezifundo zeseli ezidinga ukugcinwa ngokuphelele. Akuvamile ukusetshenziswa kwi-organic hplc ejwayelekile ngenxa yokulinganiselwa kwe-solvent.

Ukuqhathanisa ukusebenza

Ulwentshi	Ukufaneleka kwamakhemikhali	Izinga Lokugeleza	Amaprotheni abophayo	Izicelo nemikhawulo
I-PTFE	Ukumelana cishe nazo zonke izixazululo ezilonakalisayo; hydrophobic	-Ehlile	Phansi kakhulu	Izigaba ezinokhahlo; hhayi kuma-aquous ngaphandle kwe-pre-wet
Injobe	Hydrophilic; okuhle nge-ACN, DMSO; Ukuqapha nge-thf, Meoh	-Ngeqile	-Phezulu	Indlela ejwayelekile \ buffers; Gwema amaprotheni nama-acid aqinile
Uhlobo lwenhlanzi	Hydrophilic; uzinzile ngamanzi, ama-alcohols, ama-ketones	-Phezulu	-Ehlile	Amasampula e-Biological \ Gwema izisekelo eziqinile
Uhlobo	Bushelelezi; okuhle ngama-acid, usawoti, oyela; hhayi ngokoh, ama-ketones	Phezulu kakhulu	Phansi kakhulu	Izinhlayiyana \ microscopy; hhayi ejwayelekile i-hplc ye-organic

Ukukhetha ulwelwesi olufanele lwesampula yakho

Ama-solces anokhahlo noma aphezulu aphezulu + Ukulandela umkhondo: SebenzisaI-PTFEngokumelana kwayo kwamakhemikhali okungathandeki kanye nokukhishwa okuphansi.

Amasampula ezinto eziphilayo (amaprotheni) + utshwala \ amanzi: SebenzisaUhlobo lwenhlanzingeprotheni encane ebophayo kanye nokuhambisana okubanzi kwe-solvent.
I-HOSTIVE HOLLET SLOVETS:SebenzisaInjobengokuhambisana okujwayelekile nokuqina - Gwema amaprotheni noma pH extreme.
I-Particle Okubaliwe \ Microscopy: SebenzisaUhlobongosayizi oqondile we-pore kanye ne-adsorption engathandeki.

Qaphela:Njalo cabanga ngesampula yamakhemikhali, izindleko, kanye nezidingo zensimbi lapho wenza ukukhetha kwakho kokugcina.

I-AIJIREEN inikeza uhla oluphelele lokuhlunga okuphezulu kwesirinji nge-PTFE, i-nylon, pvdf, kanye nolwelwesi lwe-PC ukuhlangabezana nazo zonke izidingo zakho ze-HPLC Xhumana nathi ngamasampula noma ukubonisana kwezobuchwepheshe, futhi ake sikusize ukhethe isixazululo esifanele sokuhlunga.

Okwedlule:

Izinhlobo ze-adsortion eziphansi, ukwelashwa okungaphezulu kwe-vial, ama-vials aseLulani, ama-peg ahlanganisiwe ama-vials, ama-PFDCs Coating

Olandelayo:

Ukushisa okuphezulu kakhulu kanye nemiphumela yokuvezwa kokukhanya okuphansi kokuqina kwesampula: Ithiyori & Methol