Izinhlobo ze-adsortion eziphansi, ukwelashwa okungaphezulu kwe-vial, ama-vials aseLulani, ama-peg ahlanganisiwe ama-vials, ama-PFDCs Coating

Ngokuhlaziya okuphezulu kokuzwela, ukulahleka kwe-adsorption ezindaweni eziphansi ze-vial kungakhawulela ukunemba kokuthola. I-Intrinsic Silanol Amaqembu (si Ama-vials we-borossilate angenakwethulwa avame ukuletha izidakamizwa ze-polar noma i-biomolecule etholwa ngaphansi kwama-80%, kanye nokuhamba komsebenzi okuzenzakalelayo kwesampula kuhlushwa ukuwohloka kwesiginali okuphambili kokudonswa okuphindaphindwayo. Abathengisi batusa ama-vials we-silanized glan for ama-polar analys athambekele kwi-adsorption ye-glass, futhi ucwaningo lubonisa ngisho namasampula we-PPB-level alahlekelwa isiginali ngengilazi engalashwa kungakapheli imizuzu. Ngakho-ke, ukuhanjiswa komhlaba noma ukunamathela kubalulekile ngokunemba kwezinga le-Trace.

2. Izindawo ezisebenzayo ze-Glass nezinqubo ze-adsorption

a. Amaqembu eSilanol nama-Ion wensimbi
  Mina. Surfar si-oh amaqembu abopha ama-polar analys ngokungaguquki
  II. I-Trace Metal Ion yakha ukusebenzisana kwama-electrostatic ngama-molecule akhokhisiwe

b. Ukwethuka kwe-solvent
  Mina. I-Organic Solvents (E.G., ACN, MeOH) ingadalula izingqimba zokuhamba, iveze amasayithi amasha asebenzayo

c. Ukungcoliswa kwe-carrover
  Mina. Ama-molecule asabekayo noma ama-molecule e-hydrophilic odongeni akhiqiza iziqongo eziphakeme kuma-run alandelayo

d. Imiphumela yohlelo oluzenzakalelayo
  Mina. Imijovo ephindaphindwayo ezinhlelweni zokusebenzisa okuphezulu zikhulisa ukudonswa kwe-polar noma zokuhlaziywa kwe-trace
  II. Ukulahleka kwesiginali okubikiwe kuvame ukwedlula i-10% ngokuhamba kwesikhathi

3. Imigomo yokwelashwa engaphezulu

3.1 Ukusebenza kwendabuko

a. Ukudubula okushisa okuphezulu (~ 800 ° C)
  Mina. Unamathisela ezinye zi-oh kodwa kushiya insimbi ion ion

b. I-Acid Wash (E.G., 6 M HCL)
  Mina. Isusa i-metal ion kepha i-rose glass

c. I-Base Ukugeza (E.G., 1 M Naoh)
  Mina. Kwakha amasayithi angeziwe we-SI-O⁻, okuphikisayo

d. Ukulinganiselwa
  Mina. Ukwehliswa okuyingxenye kwezindawo ezisebenzayo ezingezansi zengilazi

3.2

a. Ukwelashwa kwe-Orgasilane ngaphansi kwe-vacuum
  Mina. I-Organesilanes (e.g., Methylnsilane) ifomu elivuselelayo le-SI-O-SI Bond ngama-silanols angaphezulu komhlaba
  II. Kwakha umgoqo we-hydrophobic omelana nokushisa, ama-acid nezisekelo
  III. I-Lower Surcace Tension futhi ibuyise ukululama kwe-Polar AnalyTTE ku-90%

b. Izibonelo Zomthengisi
  Mina. I- "DV" VIANES VIALS yokuhlaziywa kwe-polar-complic (amanzi)

3.3 ukumboza okusebenzayo

a. I-Pupluorodecylyrichlosilane (PFDCS)
  Mina. Ukuzihlanganisa i-monolayer kuthela indawo ephezulu kakhulu
  II. Ilungele ama-pahs we-nopolar pahs kanye nama-loluble ancibilikisa

b. I-Polyethylene Glycol (PEG)
  Mina. I-Hydrophilic Chains Repel amaprotheni, ama-peptides, kanye nokuhlaziywa kwamanzi
  II. Inikeza ukuvikela okuphezulu kwama-biomolecule

4. Izindlela zokulawula ama-adsorption kanye nedatha

a. Imiphumela Yemiphumela
  Mina. Izendlalelo ze-Silane zihumusha i-Glass Hydrophobic, ukuvimba ukubopha i-polar
  II. Uzinzile ngemuva kokucwiliswa okwengeziwe ku-ACN noma eMeoh

b. Ukusebenza kabusha kabusha
  Mina. Ama-vials aseLulani agcina ukutakula okuphezulu kwe-100% ye-1 PPB Doxepin ngokuhamba kwesikhathi
  II. Ama-vials e-Peg-camera afinyelela ukutakula kwama-97-99% we-polar β-lactams ngaphezulu kwe-72 h ngokuqhathanisa no-70-80% kwingilazi engalashwa
  III. I-PFDCS VIALS idlula ukutakula okungu-90% kwama-pahs kuqhathaniswa namanani aphansi aphansi esiglalweni elingenalutho

c. Izinga le-Adsortion elihlobene
  Mina. Ukuhlaziywa kwePolar: Peg> Silimane
  II. Ukuhlaziywa okungelona iqiniso

I-5. Ukukhetha uhlelo kanye nemikhuba emihle kakhulu

a. Qondanisa ukwelashwa kumakhemikhali wesampula
  Mina. Ama-Polar Compounds (izidakamizwa, amaprotheni, ama-carbohydrate): Sebenzisa izimbobo ze-silanized noma ze-peg
  II. I-Nompolar arganics (PAHS, Lipophilic Toxins): Sebenzisa izimbobo ze-PFDCS
  III. Amasampula ahlanganisiwe: I-Silanization inikeza ukusebenza okulinganiselayo

b. Cabanga nge-solvent nemvelo
  Mina. Izimbobo ze-Silane zibekezelela i-PH 1-12 ne-Organics Eningi
  II. Izambatho ze-polymer zinganciphisa ngaphansi kwama-oxidizers anamandla noma ukushisa okuphezulu; Cabanga ngokufakwa kwe-PTFE noma ama-polypropylene vials ngezimo ezimbi kakhulu

c. Isampula yevolumu ne-ivfectolovom imvamisa
  Mina. NgeMicrovolumes (<100 μl) noma isampula eliphindaphindwayo, sebenzisa izimbobo eziqinile
  II. Gada ubuqotho be-coating nge-angle yokuxhumana (> ± 10 ° Vintur uxwayisa ukwehluleka) kanye nokugijima okungenalutho (iziqongo ze-siloxane ku-m \ 28)

d. Isabelomali Sokuqinisekisa
  Mina. I-Deactivation: Izindleko eziphansi kakhulu, ezilungele izikrini zokufundisa noma ezijwayelekile
  II. Ama-siil avials: Izindleko eziphakathi nendawo, i-HPLC \ LC-MS application
  III. I-Peg \ PFDCS Coatings: Izindleko zePrimiyamu, ezilungele ama-bioanalyses abucayi futhi zilandele ukuhlolwa kwezemvelo

I-6. Isiphetho: kusuka emgodini ongabonakali esibonakalayo esisebenzayo

Njengoba umuzwa wokuhlaziya ufinyelela kumazinga we-PPB \ PPT, ama-vials wesampula abe yizindawo ezisebenzayo kunokuba iziqukathi zokuphawula. Okuhlosiwe ukwelashwa okuhlosiwe okuhlosiwe kuguqula ukulahleka okungalindeleki kube ngamapharamitha atholakalayo. Ukukhethwa kwe-vial kanye nokwelashwa komhlaba kuyizici ezibalulekile ekulinganiseni izinga eliphansi. Ngokufanisa ubuchwepheshe be-coating kumakhemikhali wesampula, ama-laboratories aguqula ama-vial abe ngamathuluzi okunemba, ukuthuthukisa kakhulu ukunemba nokuzala ngokulandela umkhondo.

Izenzo ezibalulekile

1. Kokuhlaziywa okubucayi kwe-Ultra, sebenzisa ama-vials asusiwe noma ahlanganisiwe

2. Qondanisa I-Polarity: I-Silimaned \ Peg ye-Hydrophilic, PFDCS yeHydrophobic

3. Ukuqapha i-coating: Gcina izindawo zihlanzekile, zilandelela ama-engeli othintana naye, gijimani izikhala, esikhundleni sokwehluleka

4. Ikhwalithi ye-Balance Ikhwalithi yedatha