Oct. 11th, 2023
I-Chromatography iyindlela yokuhlaziya enethonya esetshenziswa kabanzi emigqeni yesayensi, kusuka kumakhemikhali kanye nemithi emikhakheni ekuhlaziyweni kwemvelo. Ubuqotho be-Vial ngesikhathi sokuhlaziywa kwe-chromatography bubalulekile ukuze bathole imiphumela ethembekile nenembile; Lapha sihlola amathiphu abalulekile ekugcineni ubuqotho be-vial ukuze bahlole izivivinyo nge-chromatography.
1.Choose ama-vials asezingeni eliphakeme
Into ebalulekile yeqiniso le-vial lilele ekukhetheniama-vials asezingeni eliphakeme. Funa izinto ezaziwayo ngokungena kwazo njengengilazi ye-borossilte noma i-inert plastistics efana ne-polypropylene engeke isebenzisane nesampula yakho, ukuvikela ukungcoliswa kwesampula yakho, ukuvimbela ukungcoliswa kwesampula yakho. Futhi qiniseka ukuthi banobukhulu obungaguquki bokusebenzisa ama-autosamplers kanye neminye imishini ye-chromatography.
2.inspect for iziphene
Ngaphambi kokusebenzisa ama-vials, yenza ukuhlolwa okuphelele ukuthola noma yikuphi ukuqhekeka, ama-chips, noma ukungahambi kahle ezintanjeni zabo ezingaholela ekuvuzani, kungcoliswe, noma ukulahleka kwesampula ngesikhathi sokuhlaziywa. Njalo ulahle ama-vials alonakalisiwe avumela amasha ukuze alondoloze ubuqotho besampula.
3. Izinga lekhwalithi yokwesigunya ne-Septa
I-CAPS ne-Septaziyizingxenye ezihlanganayo zokugcina ubuqotho be-vial. Khetha izimboni ezisezingeni eliphakeme ne-SEPTA eyenzelwe ngqo inqubo yakho ye-Chromatography - kungaba yi-chromatography (GC) noma i-chromatography ewuketshezi (LC). Uphawu oluqinile kufanele lugcinwe ukuvikela ukuhwamuka, ukungcoliswa noma ukulahleka kwamakhompiyutha aguqukayo.
Ukuqiniswa kwe-cap e-4.propeper
Ama-cap aqiniswe kahle abucayi ukuze agweme ukuvuza nokungcola, usebenzisa noma kungaba i-torque wrench noma i-cap cristper izoqinisekisa ukuqina komfaniswano nokungaguquki; Ukuqedwa kungalimaza ama-vials ngenkathi kuqinisa ukuqina kungahle kuholele ekuhanjisweni kwesampula noma ukuvuza.
5.Kungelona indawo ehlanzekile
Indawo ehlanzekile yelabhoratri iyisihluthulelo sokunciphisa ingozi yokungcolisa ingozi. Gcina zonke izindawo zokugcina ezibonakalayo, izindawo zokusebenza kanye nemishini engenalo uthuli, ama-debris kanye nezinsalela zamakhemikhali ukuze zilondoloze ubuqotho be-vial ngenkathi ngasikhathi siqu ngokuqinisekisa ikhwalithi yokuhlaziywa.
6.Store vials kahle
Isitoreji esifanele kuyisihluthulelo sokuvikela ubuqotho be-vials. Beka endaweni epholile, eyomile kude nokukhanya kwelanga okuqondile nokuguquguquka kwamazinga okushisa ngokweqile kwemiphumela emihle. Ngokufanelekile, dala ikhibhinethi enikezelwe noma indawo ukugwema ukulimala ngengozi ngenkathi kugcinwa izimbotshana ezihlanzekile, ezingcolile.
I-7Practice ukuphathwa ngomusa
Ukusingathwa ngokucophelela kokusingathwa kwe-vial efanelekile kubalulekile ukuze kugcinwe ubuqotho babo. Gwema ukuphatha kabi futhi usebenzise amathuluzi anjengama-forceps noma amathrekhi we-vial ukunciphisa ukungcoliswa kwamafutha esikhumba kanye nezinkinga ezinhlangothini ezandleni zakho, ezingabeka engcupheni ubuqotho bazo.
Iphrothokholi yesampula yesampula
Dala futhi ulandele uhlelo olusebenzayo lwesampula oluphethe olubhekela ilebula, ukugcinwa kanye nokulandelelwa kwamasampula. Faka ilebula wonke ama-vials ngedatha ebalulekile emayelana namagama wesampula, izinsuku kanye nokugxila ukuvikela amaphutha angafanele ngesikhathi sokuhlaziywa.
9.Khetha ukuvuza
Ngaphambi nangemva kokuhlaziywa, kubalulekile ukuhlola ama-vials okuvuza. Hlola ngokombono wabo we-septa kanye nezigqoko zezimpawu zokugqoka noma ukulimala; Ngaphezu kwalokho sebenzisa imishini yokuhlola ingcindezi ukuqinisekisa uphawu oluphephile oluvimbela ukuvuza.
10. Qeqesha iqembu lakho
Yenza ukuqeqeshwa okujulile kuwo wonke amalungu eqembu lakho lakulebhuku lokuthi ungaphatha futhi ulondoloze ama-vials ngendlela efanele, ugcizelela ukubaluleka kobuqotho be-vial kanye nokulandela amaphrothokholi asungula ukulondolozwa kwawo.
11.Prevent Sampula Ukungcola
Ukungcola kungakhiqiza imiphumela edukisayo. Sebenzisa njalo izitsha ezihlanzekile, ezingamelana namakhemikhali lapho kudluliswa amasampula kuma-vials. Ngokufanelekile, lungiselela futhi uphathe amasampula endaweni ebekiwe ukuze wehlise ubungozi bokungcola.
12.pre-slit Septa
Pre-Slit Septaaklanyelwe ngokukhethekile ukuthuthukisa izinaliti zesirinji ngaphandle kokulimaza nge-Septa uqobo ngesikhathi kufakwa imijovo yesampula, ngaleyo ndlela kunciphise noma yimaphi amathuba okuhlukanisa ubuqotho obungahlehlisa ubuqotho be-vial.
13.Mainain ilebhu enembile
I-SEPTA ingaguga ngokuhamba kwesikhathi futhi iholele ezinkingeni ezifana nokuhwamuka ngempumpula kanye nokungcola, ngakho-ke kuyalulekwa ukuthi kubeyiselitiki ngezikhathi ezithile, noma ngabe zivela ngokungabonakali. Imvamisa yokubuyiselwa emuva incike ekusetshenzisweni nokuthi kuhlaziywa luni lwamasampula.
14.Minizi ukwenza ivolumu yesampula
Lapho ubhekene namasampula abalulekile noma akhawulelwe, kubalulekile ukunciphisa ivolumu yesampula ku-vial ngayinye ngangokunokwenzeka. Ukugcwala ngokweqile kungaholela ekuvuzweni kwesampula, ngenkathi ukugcwalisa kungaphansi kungavumela ukungena komoya kube yi-vials - ukuthola ukulingana kubalulekile ukuze kuqinisekiswe ubuqotho nemiphumela efanele.
15.Xoose usayizi ofanele we-vial
Ukulungiselela inqubo yevolumu nenqubo yokujova, khetha osayizi be-vial abahlangana kangcono bobabili. Ukugcwala ngokweqile kwama-vials amancane kungaholela ekusebenzeni kahle kokubekwa uphawu noma ukungcoliswa ngenkathi kukhungatheke kakhulu kungadala ukuchitheka noma ukulimala kweSepta.
16.Check Syringe Izinaliti
Hlola njalo isimo sezinaliti zesirinji ezisetshenziselwa ukujova amasampula, njengoba izinaliti ezilimele noma ezigugile zingaholela ekulimaleni kwe-Septa kanye ne-vial. Ukugcinwa kohlelo lwakho lomjovo - kufaka phakathi ukushintshwa kwenaliti ejwayelekile - kubalulekile ekuvikeleni ubuqotho be-vial.
17.
Ukuze uthole i-chromatography yegesi ephumelelayo, qinisekisa ukuthi ingcindezi yegesi yenethiwekhi ihlala ihambelana ngaso sonke isikhathi. Ukushintshashintsha kwengcindezi ekucindezelweni kungahle kuholele ekujuleni kokuvuza kanye nomonakalo weSepta; Ukuvikela ezinkingeni ezinjalo, imigqa yegesi kufanele ihlale iphephile ngaphandle kwezinkinga zokuvuza.
18.Keeep Amarekhodi
Gcina amarekhodi anemininingwane yokusetshenziswa kwe-vial kanye nokunakekelwa. Lokhu kufaka phakathi ukulandelela inani lokusetshenziswa, uhlobo lwamasampula aqoqwe kanye nesimo se-Septa nama-cap. Ngokwenza lokhu, ikuvumela ukuthi ukhombe amaphethini kusenesikhathi futhi ubhekane nezinkinga ezingaba khona ngokushesha.
19.Regularly calibrate imishini
Qinisekisa ukuthi ulinganisa imishini ye-chromatography efana nezinhlelo ze-autosamplers kanye nokujova ngezikhathi ezithile zokuqinisekisa ukuphathwa kwesampula okuncishisiwe ngengozi encishisiwe engahlehlisa ubuqotho be-vial. Ukulinganisa okufanele nakho kusebenza ukuze kugcinwe ukusebenza kahle kwemishini ngokugcina izinqubo zokuphatha isampula ezinembile.
20.Ukulawula ikhwalithi yekhwalithi
Faka izinqubo zokulawula ezisezingeni eliphezulu zokuhlola ubuqotho be-vial njengengxenye yezinqubo zelabhoratri zakho. Njalo hlola isampula yama-vials okuvuza noma ukungcoliswa kokubamba izindaba kusenesikhathi futhi uvikele ukuphindeka kwazo.
Ukugcina ubuqotho be-vial lapho ukwenza i-chromatography ukuhlaziya kubalulekile ekutholeni imiphumela eqondile nethembekile. Ngokulandela lezi zeluleko, ungathuthukisa kakhulu ukuthembeka kwenqubo yakho ye-Chromatography, okuqinisekisa idatha yekhwalithi ukuze ihlaziywe. Akunandaba noma izinga lobuchwepheshe bakho kule ndawo, lezi zindlela zizokusiza ukuba ukhiqize ngokungafanele ukuveza okuthembekile kuhlaziya ama-chromatography okuhlosiwe.
IsiNgisi
-SiShayina
