Oct. 8th, 2023
I-Chromatography iyindlela yokuhlaziya ephumelelayo esebenza ezindaweni zokuhlala ukuze uhlukanise futhi uhlaziye izingxube eziyinkimbinkimbi. Ama-chromatography vials adlala indima ebalulekile kule nqubo ngamasampula ezindlu azohlaziywa; Ukugcinwa okufanele kanye nokuphatha izindlela zalezi zinhlamvu kubalulekile ukuze uqinisekise imiphumela efanelekile evela ekuhlaziyweni kwakho. Kulesi siqondisi esiphelele sizoxoxa ngemikhuba emihle yokusingathwaAma-chromatography vialsezinhlotsheni ezahlukahlukene zesampula.
Kungani Ukulondolozwa Okufanele Nokuphatha Kubalulekile
I-Chromatogragraphy Vavials ihlukaniswe ngengilazi, esetshenziselwa ukugcina amasampula awuketshezi azohlaziywa ngamasu anjenge amasu anjenge-chromatography (LC) noma i-Chromatography (GC). Banomoya wokugwema ukungcoliseka nokuhwamuka okungenzeka ukuthi bakwazi ukuguqula imiphumela; Ngalesi sizathu uwedwa ukugcinwa okufanele futhi ukuphathwa kubalulekile ukuthola imiphumela efanele. Nakhu ukuthi yikuphi ukuphendula okufanelekile:
Ukugcina Ubuqotho Besampula:Ukugcina amasampula kuma-vials afaka izimpawu eziphephile angazivikela ekungcolisweni, ukonakala, noma ukuhwamuka - Ukusiza Ukuvimbela - Ukusiza Ukuqinisekisa ukuthi bahlala bemele umthombo wabo wezinto ezibonakalayo.
Ukuvimbela amaphutha wokuhlaziya:Ukungcola okwethulwe ngokuphatha okungafanele noma isitoreji kungangethule ukungcola kumasampula futhi kuholele ekuhlaziyweni kwama-chromatographic okulungile noma ngamanga ama-chromatographic.
Ukuphepha Ngokuphepha:Okubandakanya ochwepheshe kubalulekile lapho kulungiswa amasampula angaba yingozi noma anobuthi; Ukuphathwa kahle kwehlisa ubungozi bokuvezwa kwabahlaziyi.
1. Ukuhlanzeka kubaluleke kakhulu
Ukugcina inhlanzeko lapho ugcina ama-chromatography vials, qiniseka ukuthi ayinazo izinsalela noma ezingcolisayo ngaphambi kokubeka amasampula ngaphakathi kubo. Hlambulula usebenzisa i-solvent efanele ngaphambi kokusoma kahle ukugwema ukungcoliswa kwamasampula akho.
2. Khetha ama-vials asezingeni eliphakeme nezigqoko
Ukuvikela ubuqotho bamasampula akho, tshala imali ephezuluama-vials nama-capkusuka kubahlinzeki abathembekile. Izinketho ezishibhile zingadala ukuvuza okuhlekisayo; Ama-vials we-Glass Brossilate ajwayele ukumelana namakhemikhali futhi mancane amathuba okuxhumana nawo kunezinketho zepulasitiki.
3. Faka ama-vials ngendlela efanele
Kubalulekile ukuthi igama ngalinye libhalwe ngokucacile imininingwane ebalulekile, kufaka phakathi igama layo nosuku kanye nosuku olufanele ukunciphisa ukudideka futhi kuqinisekiswe ukuheha. Ngokulebula ngokusobala ama-vials ngalolu lwazi olunciphisa ubungozi futhi uqinisekisa ukulandelela.
4. Gcina endaweni elawulwayo
Ukugcina ukuguquguquka kokushisa nokuchayeka ku-Light okungenani, futhi kugcina amasampula angahle athintwe ukushisa noma ukukhanya ngokuhamba kwesikhathi. Lesi sinyathelo sibalulekile njengoba lezi zinto zingadala ukulimala okungenakuqhathaniswa kumasampula agcinwe lapha.
5. Gcina izimbotshana ziqonde
Gcina ama-vials aqonde ukugwema ukuvuza okungaba khona ngenkathi kukhula kanye nasekutholeni. Ukuzigcina ziqonde futhi zisiza ngenhlangano nokutholakala.
6. Gcina i-log ye-vial
Kuwukuhlakanipha ukugcina irekhodi lazo zonke izinhlobo zokusungula kwakho, ziphawula okuqukethwe kwazo, izimo zokugcina kanye nezinsuku zokuphelelwa yisikhathi ukuze ziqinisekise ukuthi amasampula asetshenziswa ngaphakathi kwezikhathi ezinconyiwe zawo futhi noma yiziphi izingqinamba ezigcinwe ngokushesha.
Imikhuba emihle yokuphatha ama-chromatography vials
1. Sebenzisa amasu afanele
Ukunciphisa ubungozi bokungcoliswa lapho kuphathwa ama-chromatography vials, ngaso sonke isikhathi landela amasu afanele ukuze wehlise. Ngokuqondile, sebenzisa ama-syringes ahlanzekile alahla noma ama-pipete lapho uhambisa amasampula futhi wenqabe ngokuthinta umgqumo we-vial ngeminwe yakho.
2. Musa ukugcwalisa
Musa ukweqa izimbotshana ze-chromatography njengoba lokhu kungaholela ekuphuleni. Qiniseka ukuthi kukhona i-headspace ezanele etholakala ekwandisweni okushisayo uma igcinwe emazingeni okushisa ahlukene, njengoba ukugcwala ngokweqile kungahle kuholele ekuqhekekeni kwe-vials ngisho nokuqhekeka kokuqukethwe kwayo.
3. Hlola ama-vials ngaphambi kokusebenzisa
Ukuqinisekisa ukusetshenziswa kwe-vial efanele, kuhlole ukuqhekeka, ama-chip, noma noma yiziphi iziphambeko ngaphambi kokwenza ukukhetha kwakho. Ama-vial alonakalisiwe kufanele achithwe ngokushesha njengoba kungaholela ezindabeni zokuvuza nezinkinga zokungcola.
4. Sivikele imishini ye-vial kahle
Ukugcina uphawu lwe-airtightI-Vial Caps, qiniseka ukuthi ziqiniswa ngokuphephile zisebenzisa i-torque wrench noma i-cap cripper yokubekwa uphawu okungaguquki nokuthembekile.
I-5. Ukuchayeka Komoya
Ukunciphisa ukucekelwa phansi kwesampula ngenxa yokuvezwa komoya, kunciphise ukuthi iziphambeko ezinde zihlala zivulekile ngaphambi kokuthola amasampula kubo. Lokhu kusiza ukuvikela ukuvezwa komoya ngokweqile ngesikhathi sokuhoxiswa kwabo.
Ukucatshangelwa okukhethekile kwamasampula abucayi
Uma isampula yakho inozwela kakhulu, isebenzisa izixazululo zokushisa ezilawulwa zokushisa ezifana neziqandisi Lapho ilebule ama-vials ukukhombisa amazinga okushisa adingekayo wesitoreji nawo ayasiza.
Amasampula abucayi okukhanyayo:Lapho usebenza ngamakhompiyutha acwebezelayo akhanyayo, sebenzisaama-amber noma ama-vials anemibalaUkuvikela amasampula kusuka ku-photodegradation futhi uyigcine ezitsheni ezimnyama noma ze-opaque ukuze unciphise ukuvezwa kokukhanya.
Amasampula we-Hygroscopic:Lapho usebenza namasampula abucayi womswakama (i-hygroscopic), sebenzisa ama-vials ngezigqoko ezifanele futhi ubheke ukusebenzisa ama-desiccants noma igesi esomile ye-nitrogen yokugcina umoya we-inert ngaphakathi kwe-vial ngayinye.
Ukuhlanza ama-vials nokulungiselela izimbotshana, nezimvilophu (nge noma ngaphandle kokubopha amanzi e-ultrapure)
Ukuhlanza Ama-Agents:Lapho uhlanza ama-vials, ama-ejenti afanele anjenge-solovents ehlanzekile aphezulu, ama-detergents noma izixazululo ze-acid ngokuya ngohlobo lokungcola kufanele zisetshenziswe njengoba kudingeka ukuhlanzwa ngokuphelele. Ngemuva kwalokho, hlanza i-vial ngayinye ngokucophelela emanzini e-ultrapure ukuqeda noma yiziphi izinsalela ezishiywe ngemuva kwabenzeli abasetshenziswe ngale nqubo.
Izindlela zokumisa:Ngaphambi kokubeka amasampula abe yi-vials, qiniseka ukuthi yonke indawo yomiswe ngokuphelele njengoba ukomisa okungaphelele kungaholela ekungcolisweni nasekuhlehliseni ubuqotho besampula.
Gwema ukungcoliswa kokuwela lapho uphatha amasampula
Ukuze uvikele ukungcoliswa kokuhamba lapho uhambisa amasampula phakathi kweziqukathi noma ama-vials, okulahlwa noma okuhlanzekile noma okuhlanzekile kwemishini ezomile kufanele kusetshenziswe lapho kuthutha amasampula akhona phakathi kweziqukathi noma izinhlobo zezinhlobo ezihlukile zesampula lapho kusebenza.
Amathuluzi Wokuphathwa Kwe-Vial:Lapho usebenza namasampula abucayi noma anobungozi, usebenzisaAmathuluzi Wokuphathwa Kwe-VialNjengama-Tweezers noma amagilavu okuphatha ama-vials angasiza ekunciphiseni ukuxhumana ngqo phakathi kwezandla nama-vials.
Izihlungi zesirinji:Lapho uthatha amasampula ezinhlotsheni, usebenzisa izihlungi zesirinji ngosayizi ofanele we-pore ukuze ususe izinhlayiyani futhi uqinisekise ukuthi ubumsulwa bamasampula akho bungasiza ekuqinisekiseni ukuthembeka kwabo nempumelelo yabo.
Nciphisa ukuvulwa kwe-vial:Okwasampula adinga ukufinyelela kaningi, ama-vial akhudlwana kufanele abhekwe ukuze anciphise ukuthi kufanele avulwe kaningi kangakanani futhi avale.
Amarekhodi okugcina kanye nemibhalo
Ngobuqotho bedatha elifanele, gcina irekhodi le-elekthronikhi lokuqukethwe kwe-vial, izimo zokugcina kanye nanoma yiluphi ushintsho ngokuhamba kwesikhathi kwisampula. Le mibhalo edijithali isebenza njengomthombo obalulekile wokulandela umkhondo.
Isoftware yokulandela umkhondo:Ama-Lab Ulwazi Lokulawulwa Kwe-Lab (Ama-Lims) noma isoftware yokulandela ngesampula iyindlela ephumelelayo futhi ephumelelayo yokubhekela ama-laboratories amakhulu anemithwalo emikhulu yesampula, ikakhulukazi lapho ukulandelela ukusungulwa kwe-vial.
Ukuqeqeshwa Nemfundo
Imfundo yayo yabasebenzi: Kubaluleke kakhulu ukuthi abasebenzi belebheli bathola ukuqeqeshwa okwanele kokuphatha okuphephile kwe-vial nokugcina. Ukubuyekezwa njalo mayelana nemikhuba emihle kakhulu kunganciphisa kakhulu ithuba lamaphutha okwenzeka emisebenzini yabo yansuku zonke.
Sebenzisa ama-protocols okuphepha: Sungula izinqubo zokuphepha lapho uphatha izinto eziyingozi, njengokubhala ngokwazo kahle futhi uzigcine ezindaweni ezibekiwe. Lapho kunesidingo, gqoka imishini yokuzivikela (i-PPE).
Isitoreji esifanele nokusingathwa kweAma-chromatography vialsukhiye wokusekela ubuqotho bedatha futhi ukhiqize imiphumela ethembekile yokuhlaziya. Ukubambelela emikhubeni engcono kakhulu mayelana nokulondolozwa okuyisisekelo nokuvikelwa kungaqinisekisa imiphumela ethembekile ye-chromatographic; Njalo bhekisa kunoma yimiphi imihlahlandlela efanelekile enikezwe ngumkhiqizi wezinsimbi zelebhu noma wokuhlaziya izinsimbi zezinsimbi zokucatshangelwa okuthile okuhlobene nohlobo lwakho lwe-vial nesampula. Ngokugcina efanelekile kanye nokuphatha imikhuba endaweni, ukuqhuba okuhlaziya okuveza ukuhlaziya okunengqondo nokunembile kwama-chromatographic ukuhlaziya kufanele kube lula kunangaphambili!
IsiNgisi
-SiShayina
