Iyini indima ye-autosampler vials ekuhlaziyweni kwe-LC-MS?

Ukuqonda ama-autosampler vials

Ama-vials we-autosampler yizitsha ezincane ezisetshenziselwa ukubamba amasampula e-liquid angeniswe ohlelweni lwe-LC-MS ukuze ihlaziywe. Lezi zinhlaka zenzelwe ukulingana ne-autosampler, ezifaka ngokuzenzakalelayo umthamo oqondile wesampula ohlelweni lwe-Chromatography System. Ukukhetha kwe-vial kungathinta kakhulu ukusebenza okuphelele kokuhlaziywa kwe-LC-MS, okuthinta izinto ezifana nobuqotho bezinsalela, ukungcoliswa, kanye nokuvela kabusha.

Vula izimfihlo zokulungiselela okulungile kwe-chromatography vial kokuhlaziywa okunembile nangokuthembekile ngezinyathelo ezi-6 ezilula. Funda ukuze ulisebenzise kahle inqubo! Izinyathelo ezi-6 zokulungiselela ama-chromatography vials ukuze ahlaziywe

Izici ezibalulekile ze-autosampler vials

Iziqukathi zesampula:Ama-autosampler vials abamba amasampula ngokuphephile ukuvikela ukungcoliswa nokuhwamuka. Idizayini ye-vial iqinisekisa ukuthi isampula ihlala iqinile ngaphambi komjovo ohlelweni lwe-LC-MS.

Ukulupheka:I-vial kumele ihambisane nohlelo lwe-autosampler ne-LC-MS. Lokhu kufaka phakathi ukucatshangelwa okufana nosayizi we-vial, ubukhulu bentamo, kanye nokuvumelana okubonakalayo okunama-solvents kanye nokuhlaziywa.

Ubuqotho beSampula:Okuqukethwe kwe-vial kungathinta ukuqina kwesampula. Isibonelo, amanye amapulasitiki angahle ahlanganise ekuhlaziyweni kwesampula noma okuhlaziya, okuholela emiphumeleni engalungile. Ama-vials engilazi avame ukuthandwa ngenxa yokuvuna kwawo.

Ukusebenziseka kalula:I-vial kufanele kube lula ukuyiphatha futhi ilayishe ku-autosampler. Izici ezifana neziqu zokulinganisa ivolumu kanye nezindawo ezicacile zokulebula zithuthukisa ukusetshenziswa.

Izinhlobo zama-autosampler vials

Kunezinhlobo eziningi ze-autosampler vials ongakhetha kuzo, ngalinye liklanyelwe izinhlelo ezithile nezidingo:

1. Ama-vials ajwayelekile

Ama-vials ajwayelekile aluhlobo olusetshenziswa kakhulu ekuhlaziyweni kwe-LC-MS. Imvamisa yenziwe ngengilazi noma ngekhwalithi ephezulu yepulasitiki futhi ifike ngobukhulu obuhlukahlukene, njenge-1 ml, 2 ml, no-4 ml. Lezi zinhlaka zilungele izinhlobo eziningi zezinhlelo zokusebenza futhi ziyahambisana nama-autosamplers amaningi.

2. Screw cap vials

Screw cap vialsube nentamo enomugqa obeka uphawu ngokuphepha nge-cap. Lo mklamo usiza ukuvimbela ukuhwamuka nokungcola. I-Screw Cap Vials ilungele amasampula aguquguqukayo noma amasampula adinga ukugcinwa isikhathi eside.

3. I-CRIMP ephezulu

Crimp top vialszibekwa uphawu nge-aluminium crimp caps enikeza uphawu lwe-airtight, ikakhulukazi amasampula abucayi emoyeni. Lezi zinhlaka zivame ukusetshenziselwa amasampula adinga ukuqina okuphezulu nokuvikelwa ekujuleni kokuwohloka.

4. Ama-Specialty Vials

Ama-vials akhethekile enzelwe izinhlelo ezithile, njengalezo ezidinga ukulungiswa kwangaphambi kwesampula noma aqukethe ukuhlaziya okusebenzayo. Isibonelo, ama-vials anokuhlunga okuhlanganisiwe kungasiza ukususa izinhlayiya ngaphambi kokujova, ngenkathi ama-vials anezinkomba ezithile anganciphisa ama-adsorption okuhlaziya.

Ufuna ukwazi ukuthi ungasikhetha kanjani i-Crimp Vial vs. Screen Vs. Screw Cap Vial ?, Bheka le ndatshana: I-Crimp Vial vs. Snap Vial Vs. Screw Cap Vial, Indlela Yokukhetha?

Ukubaluleka kokukhethwa kwe-vial ekuhlaziyweni kwe-LC-MS

Ukukhetha i-vias ye-autosampler efanelekile kubalulekile ekutholeni imiphumela eqondile nethembekile ye-LC-MS. Nazi ezinye zezinto ezibalulekile okufanele uzicabangele lapho ukhetha i-vial:

1. Ukuvumelana Kwamakhemikhali

Okuqukethwe kwe-vial kumele kuhambisane ne-solvent kanye nokuhlaziywa okuhlaziywa. Ama-vials wengilazi ngokuvamile anobuhlakani futhi alungele izinhlelo eziningi, ngenkathi amanye amapulasitiki angaxhumana nama-solvents noma ukuhlaziya, okwenza ukungcoliswa.

2.

I-vial kumele ibambe ivolumu yesampula edingekayo ukuze ihlaziywe. Ama-vial ajwayelekile ajwayele ukubamba i-1 ml kuya ku-5 ml, kepha ezinye izinhlelo zokusebenza zingadinga umthamo omkhulu. Ukuqinisekisa ukuthi ubukhulu be-vial bufanelekile ukuthi izidingo zevolumu yomjovo ye-autosampler zibalulekile.

3. Ukuvalwa kwe-vial

Indlela yokuvalwa kwe-vial ingathinta ubuqotho besampula. I-Screw cap ne-crimp cap vials anikezela ngezinketho ezahlukene zokubekwa uphawu, futhi ukukhetha kuncike ekunciphiseni nasezindaweni zokubopha uphawu lwesampula. Ukufaka uphawu kahle kahle kuvimbela ukuhwamuka nokungcoliswa ngesikhathi sokugcina nokuphatha.

4. Ukuhlanzeka kwe-VIAL

Ama-autosampler vialskumele ihlanzeke futhi ingenamahhala ezingcolisayo ezingaphazamisa ukuhlaziywa. Kunconywa ukusebenzisa ama-vials ahlanzwa ngaphambili futhi aqinisekiswe izinhlelo zokusebenza ze-LC-MS ukuqinisekisa ubuqotho bezinsampula.

5. Izindleko nokutholakala

Ngenkathi ikhwalithi ibucayi, izindleko kanye nokutholakala futhi kudlala indima ekukhetheni kwe-vial. Ama-laboratories kufanele alinganise isidingo se-vials esezingeni eliphakeme ngokucatshangelwa kwesabelomali futhi aqinisekise ukuthi ama-vial akhethiwe atholakala kalula ukuze asetshenziswe ngokuqhubekayo.

Imikhuba emihle kakhulu yokusebenzisa ama-autosampler vials ku-LC-MS

Ukuqinisekisa ukusebenza kahle ekuhlaziyweni kwe-LC-MS, cabanga ngemikhuba emihle kakhulu yokusebenzisa ama-autosampler vials:

1. Ukusingathwa okufanele nesitoreji

Phatha ama-vial ngokucophelela ukugwema ukungcola. Gqoka amagilavu ​​lapho uphatha ama-vials namasampula ukunciphisa ingozi yokwethula ama-contaminants. Gcina izipesheli endaweni ehlanzekile, eyomile ukuvikela ukonakaliswa kwesampula.

2. Sebenzisa uhlobo olufanele lwe-vial

Khetha uhlobo lwe-vial olufanele ngokususelwa kwizidingo ezithile zokuhlaziywa. Kumasampula aguqukayo, cabanga ngokusebenzisa isikulufa esivaliwe noma ama-crimp aphezulu. Okwasampula adinga ukufombula, sebenzisa ama-vials ngezihlungi ezihlanganisiwe.

3. Ukulungiswa okujwayelekile kwe-autosampler

Gcina futhi ulinganise i-autosampler njalo ukuze uqinisekise imijovo yesampula efanelekile. Izakhi ezihlanzekile ze-autosampler, kufaka phakathi inaliti kanye ne-matchection port, ukuvikela ukungcoliswa kokuwela phakathi kwamasampula.

4. Lungiselela ivolumu yesampula

Qiniseka ukuthi ivolumu yesampula ilungele umthamo womjovo we-autosampler. Ukugcwala ngokweqile noma ukugcwaliseka kwe-vial kungaholela emaphutheni ka-I-I-I-I-I-Diserk.

5. Izinqubo zokulungiswa kwesampula

Gcina amarekhodi anemininingwane yezinqubo zokulungiswa ngesampula, kufaka phakathi ukukhethwa kwe-vial, ivolumu yesampula, nanoma yikuphi ukuguqulwa okwenziwe kwiphrothokholi ejwayelekile. Leli rekhodi libalulekile ukuze lizenzeke futhi lihambisane namazinga alawulayo.

Wan ukwazi ulwazi oluphelele lokuthi ungahlanza kanjani i-Chromatography Sampula ama-vials, sicela uhlole le ndatshana: Esebenzayo! Izindlela ezi-5 zokuhlanza i-chromatography isampula ama-vials

Ukugcina

Ama-vials autosampler adlala indima ebalulekile empumelelweni yokuhlaziywa kwe-LC-MS. Idizayini yabo, ukuhambisana okubonakalayo, kanye nezinqubo zokubopha uphawu zithinta ngqo ubuqotho besampula, ubungozi bokungcolisa, kanye nokusebenza okuhlaziya okuphelele. Ngokuqonda ukubaluleka kokukhethwa kwe-vial nokunamathela kwimikhuba emihle kakhulu, ama-laboratories angathuthukisa ukuthembeka nokunemba kokuhlaziywa kwawo we-LC-MS. Njengoba izimfuno zokudlula phezulu futhi amasu okuhlaziya aqondile aqhubeka nokukhula, indima ye-autosampler vials izohlala ibalulekile ekuqinisekiseni ikhwalithi yemiphumela kuzo zonke izinhlelo zokusebenza eChristian and Emboni.