Konke odinga ukukwazi nge-HPLC Sampula Ukulungiselela

Ukulungiswa kwesampuli kuyisinyathelo esibucayi ekuhlaziyweni kwe-HPLC ngezizathu eziningi.

Ukunciphisa ukungqubuzana:Amasampula ajwayele ukuqukethe ukungcola kanye nezakhi ze-matrix ezingaphazamisa ukutholwa kokuhlaziywa okuhlosiwe. Amasu wokulungiselela isampula asebenzayo angasiza ekususeni lezi zinto ezingafuneki futhi athuthukise ukunemba kokuhlaziya.

Ngcono ukululama okuhlanzekile:Ukulungiselela okufanele kuthuthukisa ukululama kokuhlaziya kanye nokuqinisekisa ukuthi imiphumela ibonisa ukugxila kwangempela kwesampula yasekuqaleni. Lokhu kubaluleke kakhulu ekuhlaziyweni kwe-TRACE lapho kuhlaziywa khona lapho kugxila okuphansi.

Ukwakheka okuphezulu okuthuthukile:Amasampula alungiselelwe kahle asiza ekwakheni iziqongo ezichazwe kahle futhi ezichazwe kahle ku-chromatogram. Lokhu kubalulekile ekulinganiseni okunembile. Ukulungiswa kwesampula okungafanele kungaholela ekuthayimeni okuphezulu nokukhula, okubonisa ukutolika kwedatha.

Vikela uhlelo lwakho lwe-HPLC:Ukungcola kusampula yakho kungalimaza amakholomu nezinye izinto ohlelweni lwakho lwe-HPLC, okuholela ezindlekweni ezengeziwe zokulungiswa nesikhathi sokuphumula. Ukulungiswa kwesampula okufanele kunciphisa ubungozi bokuvalwa kohlelo nokugqoka.

Isinyathelo sokuqala sokulungiselela isampula ukuqoqa isampula yakho kahle. Lokhu kufaka phakathi:

Sebenzisa iziqukathi ezihlanzekile:Sebenzisa njalo uhlanzekile, u-inert iziqukathi ukugwema ukungcoliswa. Kunconywa iziqukathi zepulasitiki ezisezingeni eliphakeme.

Gwema ukungcoliseka:Sebenzisa amagilavu ​​namathuluzi ahlanzekile ukuvikela ukungcoliswa kusuka eSebum neminye imithombo.

Ukulebula okufanele:Kukhanye ngokusobala amasampula ukugwema ukuxubana futhi aqinisekise ukulandelela.

Isampula homogenization iqinisekisa ukuthi i-homogeneity, ebalulekile ukuthola imiphumela yokuvela kabusha. Amasu we-Homogenization afaka:

I-Mechan Homogenization: Ukuxuba amasampula aqinile noma aqinile asebenzisa isitha noma i-homogenizer.

I-homogenization ye-Ultrasonic:Ukusetshenziswa kwe-ultrasound ukusabalalisa izinhlayiya ngaphakathi kwesampula yoketshezi.

Ngokuya kokuqothuka okulindelekile kokuhlaziywa, amasampula angadinga ukuxutshwa noma ukugxiliswe. Lokhu kungatholakala ngo:

Dilution:Dlulisa isampula ekuhlushweni okufunayo usebenzisa i-solvent efanayo nesigaba seselula.

Ukugxila:Ukuhlaziywa kungagxila kusetshenziswa amasu anjengokuhwamuka noma ukukhishwa kwesigaba esiqinile (i-SPE).

I-Filtration ibalulekile ukuze ususe udaba olungabanjwayo olungakwazi ukuvala amakholomu we-HPLC. Izindlela ezijwayelekile zokuhlunga zifaka:

I-membrane filtration:UmsebenziIzihlungi ze-0.2μm noma ze-0.45μmukususa izinhlayiya. Imvamisa i-polyethersulfone (PES) noma izihlungi ze-nylon ziyasetshenziswa.

Ukuqanjwa:Kumasampula anokuqukethwe okuphezulu kwezinhlayiyana, okuqanjwe ngosayizi ngosayizi omkhulu we-pore kungavimbela ukuvalwa.


Imibuzo Ebuzwa Njalo Ebuzwa Ngama-Syringe, qiniseka ukuthi uhlole lesi sihloko esifundisayo: Isihloko se- "Syringe Filter" Imibuzo Ebuzwa Njalo Kaningi

Cabanga ngezinza isampula yakho ukuvikela ukonakaliswa kwe-analyte.

Ukulungiswa kwe-PH:Ukulungisa i-PH kungasiza ekuqiniseni ukuhlaziya okuthile. Isibonelo, amakhompiyutha acidic angadinga ukuguqulwa kwe-acid ukuvikela ukonakala.

Izithasiselo:Ukungeza ukuqina noma ukulondolozwa kungasiza ukugcina ubuqotho besampula ngesikhathi sokugcina nokuhlaziywa.

Kubalulekile ukukhetha i-solvent efanele ukuqeda isampula yakho futhi uqinisekise ukuhambisana nohlelo lwakho lwe-HPLC. Cabanga ngokulandelayo:

I-Solvent Polarity:Ukufeza ukuqedwa okuphelele, i-solvent kufanele ifane nobumbano lwe-analyte.

Ukuhambelana Okumayelana: Sebenzisa i-solvent ehambelana nesigaba seselula ukunciphisa ukuphazamiseka okungaba khona.

7. Ukulungiselela amazinga

Ukulungiselela amazinga okulinganisa kubalulekile ekunciphiseni i-analyte kwisampula. Lokhu kufaka phakathi:

Ukulungiselela ijika lokulinganisa:Lungiselela uchungechunge lokugxila olwaziwayo kwe-analyte ukusungula ijika lokulinganisa lokulinganisa.

Sebenzisa i-solvent efanayo:Ukugcina ukuvumelana, ngaso sonke isikhathi lungiselela amazinga akho usebenzisa i-solvent efanayo nemibandela njengamasampula akho.

Landela izinqubo zokusebenza ezijwayelekile (Sops):Sungula futhi unamathele kuma-SOPs wokulungiselela amasampula ukuze uqinisekise ukungaguquguquki kanye nokuvela kabusha.

Sebenzisa ama-reagents asezingeni eliphakeme nama-sol:Sebenzisa njalo ama-soll-ebangeni le-HPLC kanye nama-reagents amsulwa aphezulu ukuze anciphise ukungcoliswa.

Gcina indawo yakho yokusebenza ihlanzekile:Gcina indawo yakho yokulungiselela isampula ihlanzekile futhi ihleleke ukunciphisa ubungozi bokungcola.

Bhala konke:Gcina amarekhodi anemininingwane yezinqubo zakho zokulungiswa kwesampula, kufaka phakathi noma yikuphi ukuphambuka kusuka kuphrothokholi ejwayelekile.

Izindlela Zokuqinisekisa:Qinisekisa izindlela zokulungiselela isampula njalo ukuze uqinisekise ukuthi zinikeza imiphumela ethembekile nezenzekayo.

Ukwakheka okuphezulu okuphezulu:Uma iziqongo zibanzi noma zinomsila, lokhu kungakhombisa ukuqondiswa okungaphelele noma ukuba khona kwezinhlayiyana. Qinisekisa ukuthi isampula yakho ixubene kahle futhi ihlungiwe.

I-Ghost Peaks:Iziqongo ze-Ghost zingadalwa ukungcola kwi-solvent noma isampula, zisebenzise izixazululo zokuhlanza okuphezulu futhi ziqinisekise ukuthi yonke i-glassare nemishini ihlanzekile.

Ukuzwela okuphansi:Uma uzwela luphansi, cabanga ukwenza ngcono indlela yakho yokukhipha noma ukwandisa ukugxila kokuhlaziywa kwakho.

Ikholomu Ebolile:Ukuvalwa njalo kungakhombisa ukuthi isampula yakho iqukethe izinhlayiya eziningi kakhulu noma ingahlungwa ngokwanele. Hlaziya kabusha inqubo yakho ye-FILTRATION futhi ubheke ukufombula kwangaphambilini.

Imiphumela Engahambelani:Ukuhluka kwemiphumela kungahle kulungiswe amasampula angahambisani. Yenza zonke izinqubo zonke futhi uqinisekise ukuthi abasebenzi ababandakanyeka ekuhlelweni kwesampula baqeqeshiwe kahle.

Njengoba izimfuno zokuhlaziya ziyanda, ama-laboratories amukela amasu athuthukile wokulungiselela amasampula ukuthuthukisa ukusebenza kahle nokunemba:

I-SPE iyindlela esetshenziswa kabanzi evumela ukukhishwa okukhethiwe kokuhlaziywa kusuka kumatikuletsheni okuyinkimbinkimbi. Kubandakanya ukudlula isampula yoketshezi ngokusebenzisa impahla eqinile e-adsorbent egcina umhlaziyi welitshe ngenkathi evumela ukungcola ukuthi kudlule.

I-Lle yindlela ehlukanisa ama-analytes asuselwa kwi-solubenity yabo oketsheni ezimbili ezingenangqondo. Le ndlela iyasebenza ekuhlukaniseni amakhompiyutha ane-polarities ehlukile futhi ivame ukusetshenziswa ekuhlaziyweni kwezemvelo nezemithi.

I-Quechers iyindlela edumile yokulungiselela amasampula wokudla namasampula ezemvelo ahlanganisa izinyathelo zokukhishwa kanye nezinyathelo zokuhlanza zibe yinqubo eyodwa, okwenza kusebenze futhi kubiza kakhulu.

Ukudluliswa kabusha kanye nokuvela kabusha kungathuthukiswa kakhulu ngezinhlelo ezenzakalelayo zokulungiselela isampula, ezingenza imisebenzi efana ne-dilution, i-filtration, kanye ne-spe ngokungenelela komuntu okuncane, ngaleyo ndlela kunciphise ubungozi bamaphutha.


Ufuna ukwazi kabanzi nge-HPLC Sampula Ukulungiselela, sicela ubheke le ndatshana: I-HPLC isampula izixazululo zokulungiselela imiphumela emihle kakhulu

Ukulungiswa kwesampuli ye-HPLC kuyisinyathelo esibucayi esingathinta kakhulu ukunemba kanye nokwethenjwa kwemiphumela yokuhlaziya. Ngokulandela imikhuba emihle kakhulu, kusetshenziswa amasu afanele, futhi ukubhekana nezinkinga ezijwayelekile, ama-laboratories angakhulisa inqubo yokulungiselela isampula ngokwamukela amasu athuthukile nobuchwepheshe njengoba izidingo zokuhlaziya zivela. I-automation izothuthukisa ukusebenza kahle kanye nokusebenza ngempumelelo kokuhlaziywa kwe-HPLC. Ukulungiselela okwesampula okufanele akuvezi kuphela ikhwalithi yemiphumela, kepha futhi kuvikela uhlelo lwe-HPLC, kuqinisekise ukwethenjwa kanye nokusebenza kwesikhathi eside.