Amasu ajwayelekile wokulungiselela amasampula we-GC-MS

I-Gesi Chromatography-Mass Spectrometry (GC-MS) iyindlela enamandla yokuhlaziya esetshenziselwa ukuhlaziya amakhompiyutha aguqukayo kanye nama-semivolatile. Ngokuya ngohlobo lokuhlaziywa kwesampula kanye nelitshe, izindlela ezahlukahlukene zingasetshenziswa ukuze zilungiselele ngempumelelo isampula. Okulandelayo amasu ajwayelekile asetshenziselwa ukulungiselela amasampulaUkuhlaziywa kwe-GC-MS:

Ufuna ukwazi kabanzi ngomehluko phakathi kwe-LC-MS ne-GC-MS, sicela uhlole le ndatshana:Uyini umehluko phakathi kwe-LC-MS ne-GC-MS?

1. Ukulungiswa kwesampula ye-Liquid

I-Dilution: Amasampula e-Liquid ahlanjululwe ngokujwayelekile ephuzwini elibi elibi kakhulu njenge-methanol, i-acetone, noma i-dichloromethane ukufezekisa ukugxila okucishe kube ngu-0.1 kuya ku-1 mg \ ml. Lokhu kuqinisekisa ukuthi isampula iyahambisana nohlelo lwe-GC futhi inciphisa ubungozi bokuvalelisa i-inlet.

I-FILTRATION: Ngaphambi kokuhlaziywa, isampula kufanele ihlungwe ukususa noma yiziphi izinhlayiya ezingaphazamisa ukuhlaziywa. AIsihlungi se-0.22 μmkuvame ukusetshenziswa.

I-CentriFugation: Amasampula angaqukatha ama-solids, i-centrifugation angasiza ukuhlukanisa uketshezi kunoma iyiphi impahla engaqondile ngaphambi kokudlulisela ku-vial.

2. Ukulungiswa kwesampula okuqinile

Ukuchithwa: Amasampula aqinile kufanele achithwe endaweni ephansi ephansi ye-solvent ephansi. Ngeza inani elincane (okusanhlamvu okumbalwa) kokuqinile kuya komoya we-solvent futhi ungenwe amahlandla ambalwa ukuqinisekisa ukuchithwa okuphelele.

Ukutholwa: Kwezinhlanganisela ze-Semi-Volatile noma ama-Polar, ukufunwa kwe-polar kungadingeka ukuthuthukisa ukuguquguquka futhi kuthuthukise ukuzwela kokutholwa. Lokhu kufaka ukulungisa amakhemikhali ukuguqula i-analyte ukuze kwenze kube lula kakhulu ekuhlaziyweni kwe-GC.

3. Ukuhlaziywa kwekhanda

I-Static Headspace: Ngale ndlela, i-vial ebekwe uphawu equkethe isampula ibanjelwa ekushiseni okuhlala njalo ukuvumela amakhompiyutha aguqukayo ukuba asebenzise i-headpace ngenhla kwesampula ngaphezulu kwesampula ngaphezulu kwesampula ngaphezulu kwesampula. Lapho ukulingana kufinyelelwa, lekhanda lekhanda lingakhishwa ukuze lihlaziywe lisebenzisa isirinji eqinile egesi.

I-Dynamic HeadSpace (Hursge kanye nesicupho): Le ndlela ifaka ukudlula igesi i-inert ngokusebenzisa isampula ukuthuthukisa ukukhishwa kwezinto eziguqukayo e-headpace. Le ndlela inyuka kakhulu ukuzwela ngokugxila kwama-volutiles ngaphambi kokuhlaziywa.

Ufuna ukwazi kabanzi ukuthi kungani ama-headpace vials asetshenziswa ku-chromatography ?, Sicela ubheke le Artice: Kungani ama-headspace vials asetshenziswa ku-chromatography? 12 engles

4. Amasu wokukhipha

Isigaba esiqinile sesigaba se-microextraction (SPME): I-SPME isebenzisa i-fiber ehlanganiswe nesigaba sokukhipha ukudonsa i-analyte kusuka esigabeni noma ephepheni. Le ndlela ivumela isampula eqondile ngaphandle kwesidingo sangemuva futhi iwusizo ikakhulukazi kumakhompiyutha aguqukayo.

Ukukhishwa okuyisikhilimu (i-Lle) nesizinda sesigaba esiqinile (i-SPE): Lezi zindlela zisetshenziselwa ukuhlanza amasampula ngokuhlukanisa ama-analytes ezintweni ezihlangene kuma-matric ayinkimbinkimbi ngaphambi kokuhlaziywa kwe-GC-MS.

5. Amathiphu wokugxila

I-nitrogen ihlanza: Le ndlela isetshenziselwa ukugxilisa amasampula ngokuhwamuka kwezixazululo ngaphansi komfula we-nitrogen, esiza ukunciphisa ivolumu yesampula ngenkathi kugcinwa ukuhlaziya ngenkathi kugcina ukuhlaziya.

Ukuhlolwa Kwesampula Ukulungiselela

Qinisekisa ukuthi wonke ama-solvents asetshenziswe aguquguqukayo futhi alungele i-GC-MS; Amanzi nama-solvents angenamanzi kufanele agwenywe.

Amasampula akumele aqukethe noma yimaphi ama-acid aqinile, izisekelo, usawoti, noma ezinye izinto ezingcolisayo ezingalimaza ikholomu ye-GC noma ziphazamise ukuhlaziya.

Amasampula wokugcina kufanele abe mahhala yezinhlayiya futhi mhlawumbe zilungiselelwe ngaphakathiizingilazi zeglasi ukuvikela ukufakwa kwezinto zokwakha kusuka epulasitiki.

Ngolwazi oluthe xaxa ngama-autosampler vials we-chromatography yegesi, bhekani le ndatshana: 2 ml autosampler vials we-chromatography yegesi

Ukugcina

Amasu wokulungiselela isampula asebenzayo abalulekile ekuhlaziyweni kwe-GC-MS ephumelelayo. Indlela ngayinye inezinzuzo zayo futhi inezinhlelo ezithile ngokuya ngohlobo lwesampula nelitshe. Ngokusebenzisa la masu, abahlaziyi bangathuthukisa ukunemba, ukuzwela, kanye nokuzala kwemiphumela yabo, ekugcineni ukuthola idatha ethembekile emikhakheni ehlukahlukene njengokuqapha imvelo, ukuphepha kwemithi kanye nemithi.