I-Gesi Chromatography-Mass Spectrometry (GC-MS) kanye ne-Chromatography ephezulu yokusebenza kwe-chromatography (HPLC) ngamasu amakhulu amahle wokuhlaziya asetshenziselwa ukuhlukanisa, ukukhomba, kanye nezinhlanganisela zamasampula ahlukahlukene. Indlela ngayinye inezinzuzo zayo ezihlukile futhi ilungele izinhlobo ezahlukene zokuhlaziywa. Ukuqonda umehluko oyisisekelo phakathi kwe-GC-MS ne-HPLC kubalulekile ekukhetheni inqubo efanelekile ngokususelwa kuhlobo lwesampula kanye nezidingo ezithile zokuhlaziya.

Ufuna ukwazi izimpendulo ezingama-50 mayelana ne-HPLC VIALS, sicela uhlole le ndatshana:Imibuzo evame ukubuzwa kaningi ku-HPLC VIALS

Umehluko omkhulu phakathi kwe-GC-MS ne-HPLC

1. Isigaba seselula

Umehluko omkhulu phakathi kwe-GC-MS ne-HPLC isigaba se-mobile. I-GC -S isebenzisa isigaba se-Gaseous Mobile Mobile, imvamisa igesi e-inert enjenge-helium noma i-nitrogen, ukuthutha isampula elenziwe ngekholomu ye-chromatogated. Lokhu kwenza ukuthi i-GC-MS ilungele ikakhulukazi ukuhlaziya amakhompiyutha aguqukayo avele kalula emazingeni okushisa aphezulu.

Ngokuphambene nalokho, i-HPLC isebenzisa isigaba se-liquid mobile, imvamisa ingxube ye-solvent ehambelana nobumbano kanye nokulimazeka kwesampula. Lokhu kwenza ukuthi i-HPLC ihlaziye uhla olubanzi lwezinhlanganisela, kufaka phakathi izinto eziguquguqukayo nezingezinhle.

2. Uhlobo lwesampula

Izinhlobo zamasampula ezingahlaziywa ngenqubo ngayinye ziyehluka kakhulu. I-GC-MS ilungele kangcono ukuhlaziya amakhompiyutha aguquguqukayo noma aguquguqukayo, anjengeHydrocarbons, uwoyela obalulekile, kanye nokungcoliswa kwemvelo. Isebenza kangako amakhompiyutha okuhlanza ukushisa noma okungaguquki. I-HPLC, ngakolunye uhlangothi, ingaphatha amasampula abanzi, kufaka phakathi ama-poomor compound, i-biomoleks, amakhamishini, kanye nokuxuba okuyinkimbinkimbi okungaqukatha usawoti noma izinhlobo ezikhokhiswayo. Lokhu kuguqulwa kwenza i-HPLC ibe ngukukhetha okuphezulu emikhakheni efana ne-biochemistry kanye nemithi yezemithi.

Wan ukwazi ulwazi oluphelele lokuthi ungahlanza kanjani i-Chromatography Sampula ama-vials, sicela uhlole le ndatshana:Esebenzayo! Izindlela ezi-5 zokuhlanza i-chromatography isampula ama-vials

3. Izimo zokushisa

Ukushisa kudlala indima ebalulekile kuwo womabili amasu, kodwa ngezindlela ezihlukile. I-GC-MS isebenza emazingeni okushisa aphezulu kakhulu, imvamisa phakathi kuka-150 ° C no-300 ° C, ukuqinisekisa ukuhwamuka okusebenzayo kwesampula. Lesi sidingo sokushisa esiphakeme sivumela ukuhlaziya ngokushesha, kepha sikhawulela izinhlobo zamasampula angahlaziywa, njengoba amakhompiyutha abucayi angenza. Ngokuphambene nalokho, i-HPLC yenziwa ngokujwayelekile kumazinga okushisa noma aphakanyiselwe kancane, okwenza kulungele ukuhlaziya amakhompiyutha acwebezelayo okushisa ngaphandle kwengozi yokubola.

4. Indlela yokwehlukanisa

I-GC-MS ne-HPLC inezindlela ezahlukahlukene zokwahlukana ngenxa yezigaba ezihlukile zeselula. Ku-GC-MS, ukwahlukana kusekelwe ikakhulu ekuguqukeni kwamakhompiyutha; Amakhompiyutha amancane aguqukayo asebenzisana kakhulu nesigaba esimisiwe kanye nokuvutha okuthe xaxa kunamakhompiyutha aguquguqukayo ngokwengeziwe.

Ngokuphambene nalokho, i-HPLC ihlukanisa amakhompiyutha ngokususelwa ekusebenzisaneni kwawo ngezigaba zeselula nezimisayo, ezinqunywa yizinto ezifana ne-polarity kanye ne-solubity. Amakhompiyutha e-polar ajwayele ukuhambisa ikholomu ngokushesha ngoba akhangwa kakhulu esigabeni seselula.

5. Izindlela zokutholwa

Izindlela zokutholwa eziqashwe yi-GC-MS ne-HPLC nazo zihluke kakhulu. I-GC -S ihlanganisa i-chromatography yegesi nge-mass spectrometry, okuvumela ukutholwa okubucayi kakhulu nokukhonjwa kwamakhompiyutha ngokususelwa ku-ratio yabo yokukhokhisa kakhulu ngemuva kokuhlukaniswa. Le nhlanganisela ihlinzeka ngemininingwane enemininingwane yesakhiwo mayelana nokuhlaziywa. Ngokungqubuzanayo,I-HPLCImvamisa isebenzisa i-UV-ebonakalayo spectrophotometry noma umtshina wenkomba owesabekayo, olinganisa ukuthi isampula imunca noma ishintsha kanjani izakhiwo ezilula njengoba idlula emtshinini. Ngenkathi lezi zindlela zisebenza kwizicelo eziningi, zinganikeza imininingwane engaphansi yesakhiwo kune-spectrometry eningi.

I-6. Imishini Nezindleko Ukucatshangelwa

Imishini edingekayo ye-GC-MS ne-HPLC futhi ihluka kakhulu ngokwemigomo yobunzima nezindleko. Amasistimu we-GC ngokuvamile alula; Badinga ukulethwa kwegesi (igesi yenethiwekhi) kodwa hhayi iphampu yengcindezi ephezulu ngoba amagesi anokuvunywa okuphansi kunokuphuza. Lokhu ngokuvamile kwenza izinhlelo ze-GC zibiza kakhulu ukusebenza esikhathini eside. Ngokuphambene nalokho, amasistimu we-HPLC adinga iphampu yengcindezelo ephezulu yokucindezela i-solvent ewuketshezi ngekholamu eligcwele isigaba esimisiwe, futhi ibiza kakhulu ukuze ilondoloze ngenxa yesidingo sohlelo olukhethekile.

Ukukhetha phakathi kwe-GC-MS ne-HPLC

Lapho unquma ukuthi usebenzise i-GC-MS noma i-HPLC, kunezici eziningana okufanele uzicabangele:

Uhlobo lwesampula yakho: Thola ukuthi isampula yakho iguquguqukayo noma i-nonvolatile.

Ukuqina okushisayo: Hlola ukuthi ama-analytes akho angamelana namazinga okushisa aphezulu ngaphandle kokonakaliswa.

Ukuzwela okudingekayo: Bheka ukuthi udinga imininingwane yezakhiwo enemininingwane (ethanda izilinganiso ze-GC-MS) noma izilinganiso zokuhlushwa kuphela (ezingenziwa nge-HPLC).

Izingqinamba zezindleko: Hlola isabelomali sakho ukuze uthenge noma kulungiswa.

Ngamafuphi, womabili ama-GC-MS ne-HPLC angamathuluzi abaluleke kakhulu kumakhemikhali wokuhlaziya, futhi indlela ngayinye inezinzuzo zezicelo ezithile. Ngokuqonda umehluko wabo oyisisekelo (isb.

Ufuna ukwazi kabanzi ngomehluko phakathi kwe-LC-MS ne-GC-MS, sicela uhlole le ndatshana:Uyini umehluko phakathi kwe-LC-MS ne-GC-MS?

Okwedlule:

Uyini umthelela wosayizi we-vial kwimiphumela ye-chromatography?

Olandelayo:

Amasu ajwayelekile wokulungiselela amasampula we-GC-MS

Amathegi: I-Gaschcromatography I-Chromatography-vials I-Chromatography Igesi-chromatography-vials Hplcananisalsis I-HPLC-VIALS

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