Ngabe uyazi ngekwelashwa kweSulanization ye-HPLC Vial?

Silanization of HPLC VIALSinqubo ebalulekile eyenzelwe ukuthuthukisa ukusebenza kwe-chromatographic ngokunciphisa ukunqwabelana kokuhlaziywa kwendawo yengilazi ye-vial. Lokhu kwelashwa kuzuzisa ikakhulukazi ukuhlaziya ngobuningi, ikakhulukazi lapho ubhekene namasampula aphansi, njengoba noma yisiphi isampula esisele singayithinta kakhulu imiphumela. Le ndatshana ihlinzeka ngesibukezo esiphelele senqubo yokuhlanza, izinzuzo zayo, izinhlelo zokusebenza, kanye nokucatshangelwa kokusetshenziswa ku-chromatography ephezulu ye-chromatomatography (HPLC).

Kuyini i-silanization?

I-silanization ifaka ukusebenzisa i-sackond ye-sane yangaphakathi ye-vial yengilazi ye-borossilte. Le nqubo ngempumelelo isebenze amaqembu we-hydroxyl ephumelelayo Ngokuhlikihla lezi zingosi ezisebenzayo, i-silanization inciphisa kakhulu ama-adsorption okuhlaziya ezindongeni ze-vial, ngaleyo ndlela aqinisekise ukutholakala okuphezulu nemiphumela yokuhlaziya enembile kakhudlwana. Ku-HPLC VIALS, lokhu kwelashwa ngokuyinhloko kuhlose amaqembu e-silanol (si-oh) endaweni engaphakathi yengilazi ye-borossiling. Lawa maqembu ama-hydroxyl angasebenzisana nokuhlaziywa kwePolar, okuholela ekusakazeni ama-adsorption kanye nokulahleka ngesikhathi sokuhlaziywa.

Inqubo yokusanzanzanga

Inqubo yokuhlanganisa i-silanization ngokuvamile ifaka lezi zinyathelo ezilandelayo:

Ukuhlanza: Okokuqala, hlanza indawo yengilazi kahle usebenzisa i-organic solvent enjenge-acetone ukuze ususe noma yikuphi ukungcola okungaphazamisa i-silanization.

Ukusetshenziswa kweSilane: Umenzeli we-methyl silaning wangeniswa endaweni yangaphakathi ye-vial nge-Vapor Deposition. Umenzeli uphendula ngamaqembu e-hydroxyl ukwakha ungqimba oluboshelwe oboshwe nge-hydrophobic olungasebenzi kahle futhi oluncane lwe-adsorsorptive.

Ukubopha Okuhehayo: USilane uphendula ngamaqembu e-hydroxyl endaweni yengilazi ukwakha ungqimba lwe-hydrophobic, ukunciphisa ukuvumelanisa kanye nokuvimbela ukuhlaziya kusuka ekutholeni izindonga ze-vial.

Ukwelapha: Amabhodlela aphathisiwe ngokuvamile ayelapheka ukuze aqinisekise ukuthi isilonda sakha isibopho esizinzile esiya ebusweni bengilazi, ngaleyo ndlela sikhule ukuqina okuxakile ku-solvents nezinye izimo ezihlangabezana nazo ngesikhathi se-chromatography.

Umphumela uba yi-vial enomugqa oguquliwe onciphise ukusebenzisana ngezakhi ze-polar, ethuthukisa ukululama kwesampula kanye nokunemba kokuhlaziya.

Izinzuzo ze-silanization

Kusetshenziswa ama-HILISED HPLC Vavials anikeza izinzuzo ezibalulekile:

1. Thuthukisa ukuvuselelwa kwesampula

Ama-vials aseLulani anciphisa kakhulu ukunamathela kokuhlaziywa kwezindonga ezibonakalayo. Lokhu kubaluleke kakhulu ekuhlaziyweni kokuhlushwa okuphansi, lapho kulahleka khona isampula yesampula. Ngokuvimbela ama-adsorption, ama-vials aseLulani aqinisekisa ukululama okuphezulu kokuhlaziywa.

2. Thuthukisa ukunemba

Lokhu kuphamba kuqinisekisa ukuthi imiphumela ayithinteki ekunamatheleni okusalayo okunamathela ebusweni be-vial, ngaleyo ndlela yenza ngcono ukunemba okulingana okubalulekile ekuhlaziyweni ngobuningi lapho ukunemba kubalulekile.

3. Ukuhambisana nokuhlaziywa okuhlukahlukene

Ama-vials aseLulani alungele izinhlelo ezahlukahlukene, kufaka phakathi lezo ezibandakanya amakhompiyutha wePolar neHydrophobic. Zilungele ikakhulukazi ama-Pharmaceuticals, Biopolymers nezinye izinto ezibucayi ezingase zisebenzisane kabi nge-Standard Glass Surface.

4. Impilo ende eshalofini

Inqubo yesiSulanization yesigaba se-Vapor ikhiqiza izimbobo ezizinzile ezigcina izakhiwo zazo ngokuhamba kwesikhathi. Ama-sailings aseLulanised ahlala ezinzile isikhathi eside noma ngabe kuvezwa ama-solvents ahlukahlukene futhi alungele ukugcinwa isikhathi eside.

Izicelo ze-silanised HPLC VIALS

Ama-vials e-HILISED HPLC angasetshenziswa emikhakheni ehlukahlukene:

1. Ukuhlaziywa kwezidakamizwa

Ema-laboratorics wezemithi, kusetshenziselwa izimbotshana ze-silanizen:

Ukuhlolwa kokuqina: Ukuqinisekisa ukuthi ukwakheka kwezidakamizwa kuhlala kuzinzile ngesikhathi sokugcina nokuhlaziywa.

Ukuhlaziywa kwesilinganiso: kusiza ukukala ngokunembile ukugxila kwezidakamizwa ekwakhekeni.

2. Ukuhlolwa Kwezemvelo

Lezi zinhlaka zibalulekile ekuqapheleni kwemvelo:

Ukuhlolwa Kwekhwalithi Yamanzi: Hlaziya amasampula wamanzi ukuze angcoliswe ngenkathi unciphisa ukulahleka kwe-adsorption.

Ukuhlaziywa kwenhlabathi okukhishwe kwenhlabathi: lungiselela ukukhishwa kwenhlabathi ngokuhlaziywa kwamakhemikhali ngaphandle kokulahlekelwa ukuhlaziya okuhlosiwe.

3. Ukuhlolwa kokuphepha kokudla

Kwizicelo zokuphepha zokudla:

Ukuhlaziywa kwezinsalela zokubulala izinambuzane: Qinisekisa ukutholwa okunembile kwamazinga ezinambuzane ekudleni ngokuvimbela ukulahleka kwesampula ngesikhathi senqubo yokuhlaziya.

Ukuhlolwa kokuqukethwe kwezakhi zomsoco: Hlaziya amasampula wokudla wokuqukethwe kwezakhi ngaphandle kokuphazamiseka kusuka ekuxhumaneni kwe-vial.

4. Ucwaningo Nentuthuko

Esimweni sokucwaninga:

Ukulungiswa kwesampula: Kuhlinzeka ngezixazululo zokugcina ezithembekile zezinhlobo ezahlukahlukene zawo ngaphambi kokuhlaziywa.

Izicelo Zokuhlola: Kusiza izivivinyo ezibandakanya amakhompiyutha abucayi adinga izimo ngawo.

Izindlela zokuphepha lapho usebenzisa ama-vials aseliva

Ngenkathi ama-vials e-silani anikela ngezinzuzo eziningi, kunezinto ezithile ezibalulekile okufanele ziqaphele:

1. Khetha uhlobo lwe-pastle yesampula efanele

Kubalulekile ukuthi ukhethe uhlobo olufanele lwe-vial ye-silanized yesidingo sakho sohlelo lokusebenza. Izici ezifana nevolumu yesampula, ukuhambisana kwamakhemikhali, kanye nokuzwela okufiselekayo kufanele kuqondise ukukhetha.

2. Izinyathelo zokusebenzisa ukusetshenziswa

Ukwelashwa okufanele kubalulekile ukuze kugcinwe ubuqotho bendawo ebelini ye-silanized:

Gwema ukuxhumana ngqo nezindawo ezingaphakathi ukuvikela ukungcoliswa.

Gqoka amagilavu ​​ahlanzekile lapho uphatha izipesheli ukunciphisa ukudluliswa kwezinsalela.

3. Ukuhambisana nama-solvents

Ngenkathi izindawo ezihlasimuli zasesilivali zivame ukumelana nama-sol amaningi, kubalulekile ukuqinisekisa ukuhambisana namakhemikhali athile asetshenziswe ekuhlaziyeni ukuvikela ukonakala noma ukulahleka kokusebenza.

Ukwelashwa okuhlukile

Ngenkathi i-silanization isebenza kahle, kunezinye izindlela ezinjengezinto ezincishisiwe zomsebenzi (RSA). Ama-RSA Vavials aklanyelwe ukuba abe nomsebenzi ophansi wemvelo futhi awathembeli embonweni kaIlane. Le ndlela inganikeza imiphumela engaguquki eminingi ukuthola izinhlelo ezithile lapho kulungiswa i-silanization yendabuko ngenxa yokuthola imali engaphelele noma ukucekelwa phansi ngokuhamba kwesikhathi.

Uma ufuna ukwazi kabanzi ngama-HILICEDED VPLC VIALS, sicela uchofoze le ndatshana:"Isethulo kuma-HILICEDISIWES VPLC VIALS"

I-Silanization ye-HPLC Vials iyinqubo ebalulekile ethuthukisa ukusebenza kokuhlaziya ngokunciphisa ukubhekwa kokuhlaziya nokwanda kwesampula. Ngokushintsha indawo yangaphakathi ye-BOROSILiLil Glass Vials, lokhu kwelashwa kuqinisekisa imiphumela enembile, ethembekile ezinhlobonhlobo zezicelo ekuhlolweni kwemithi, ukuhlolwa kwezemvelo, ukuphepha kokudla, kanye nezilungiselelo zocwaningo. Ngokuqonda izinzuzo kanye nokucatshangelwa okufanelekile okuhambisana nama-vials aselivalisiwe, ama-laboratories angakhulisa ngokuphumelelayo ukugeleza komsebenzi wawo wokuhlaziya ngenkathi egcina amazinga asezingeni eliphakeme kanye namazinga okunemba ukuze uthole imiphumela. Njengoba ubuchwepheshe be-silanization buqhubeka buqhubekisa phambili, abacwaningi bangalindela ukuthuthuka okuqhubekayo ekusebenzeni kwe-vial kanye nokusebenza kuyo yonke imiyalo yesayensi ehlukahlukene.