I-Chromatography iyindlela yokuhlaziya ebabazekayo esetshenziswa ezindaweni zokuhlaziya izicelo ezahlukahlukene zokuhlaziya, kufaka phakathi ukubona, ukuhlukanisa, kanye namakhompiyutha amakhemikhali akhona akhona ekuxubeni okuyinkimbinkimbi. Ukuqinisekisa imiphumela eqondile nethembekile ye-chromatographic imiphumela, ukhetha ama-vials angu-18mm - usayizi ojwayelekile kuzo zonke izinhlelo zelebhu - ubalulekile. Lapha sihlola ukucatshangelwa okubalulekile kokukhetha lapho ukhetha lolu hlobo lwe-vial.

1. Izinto zokwenziwa kanye nokufaneleka kwamakhemikhali

Ukucatshangelwa ngokwezinto ezibonakalayo lapho ukhethaI-18mm chromatography vialskungenye ebaluleke kakhulu. Ukukhetha okujwayelekile kufaka ingilazi namapulasitiki ahlukahlukene njenge-polypropylene ne-polyethylene; Lapho ukhetha ama-vials wokuhlola kwakho kubalulekile futhi ukuthi aqondanise namasampula nama-sol asetshenziswa.

I-Glass Vials:Izingilazi zeglasizilungele izinhlelo zokusebenza ezibandakanya amasampula aphezulu okushisa noma izixazululo ezinolaka, njengoba zinobudlova futhi zingabekezelela amazinga okushisa ahlukahlukene nezimo zemvelo. Bangase futhi basebenzise ama-adderb aqonde epulasitiki eyenza ukulahlwa okulula.

Ama-Vials epulasitiki:Ama-vials epulasitikiNikeza izixazululo ezingavikezi ezingasindi futhi ezishisayo, ezibenza babe inketho ekhangayo yezinhlelo ezithile. Kodwa-ke, abacwaningi kufanele baqiniseke ukuthi ipulasitiki yabo ekhethiwe iyahambisana ne-solvents noma amasampula asetshenziswayo, ukuvikela ukuqondisisa noma ukungcoliswa kwamasampula nama-sol.

I-Solvent-System Ukuqhathanisa Matrix

Uhlobo lwe-polymer	ububanzi be-PH	Ukubekezelela Ukucindezelwa	Ukusetshenziswa okukhethekile
I-Polypropylene (PP)	1-12	Ibha engu-600	Izigaba zeselula ze-LC-MS ezine-ion-pair reagents (0.1% TFA)
I-Polyeetheketone (Peek)	0-14	> I-1000 Bar	Amasistimu we-UHPLC (i-acetonitrile ukuvuvukala <0.5% ngaphezulu kwe-72h)
Okuhlukahlukene Okuguquguqukayo	N \ a	Ibha engu-400	Ukuhlaziywa kwe-Peptide (ama-20-30% ukuthuthukiswa kokulungisa ama-vs angenziwa)

2. Ukuvumelana phakathi kokufakwa kwe-cap ne-septa

I-CAP ne-SeptaUkukhetha kubaluleke kakhulu ku-18mm chromatography vials njengokukhethwa kwezinto ezibonakalayo. Basebenza njengezithiyo eziphathelene nokungcoliswa kwesampula kanye nokuhwamuka, ngakho-ke cabanga ngalezi zinto ngaphambi kokukhetha kwakho:

Izinhlobo ze-Cap: Kunezinhlobo ezahlukahlukene ze-cap, ezifana nama-screw cap, ama-crimp cap kanye nama-snap cap, angakwazi ukuhambisana nemishini yakho yelabhoratri namasu. Kubeka phambili ukuhambisana lapho ukhetha eyodwa ukuze uyisebenzise.

Izinto zeSepta: I-Septa yakhiwa ngokujwayelekile kusetshenziswa izinto ezifana ne-polytetrafluoroethylene (PTFE) noma i-silicone, ngakho-ke qiniseka ukuthi lowo okhethiwe avumelana namasampula nama-sols ohlela ukukuhlola. I-PTFE ihlinzeka ngokumelana kahle kwamakhemikhali ngenkathi ama-silicone Setals anikeza izakhiwo ezinokwethenjelwa zokubekwa uphawu.

3. Izicelo futhi usebenzise amacala

Icala lokusebenzisa nokusebenzisa likucatshangelwa okubili okubucayi lapho ukhetha ama-vials angu-18mm chromatography vials. Faka emqondweni:

IVampula yesampula: Lapho ugcwalisa ama-vials ngamasampula wokuhlaziywa, aqiniseke ukuthi amukela ivolumu yesampula edingekayo. Ukugcwala ngokweqile noma ukugcwaliseka kungahlehlisa ukunemba kwemiphumela ye-chromatogagraph.

Indlela yokuhlaziya: Amasu ahlukene we-chromatographic, njengegesi ye-chromatography (GC) kanye ne-chromatography ewuketshezi (LC), adinga izinhlobo ezithile noma izinto zokwakha; Qaphela izidingo zakho ezikhethiwe ngaphambi kokuqhubeka.

Uhlobo lwesampula: Izakhiwo Zamakhemikhali Zakho Zama-Samples kanye namathuba okuba ama-adsorption azothonya izinto zokwelashwa ezitholakalayo kuwe lapho ukhetha izinto zokwelapha kanye nokwelashwa komhlaba ngezinto zokwelashwa.

Izidingo zokubopha uphawu: Bheka ukuthi uhlelo lwakho lokusebenza ludinga izimpawu zomoya zokuvikela ukuhwamuka kwesampula noma ukungcoliswa.

Lapho ukhetha ama-vials angu-18mm Chromatography Vavilogs, abacwaningi kufanele bacabangele ngokucophelela ukuhambisana okubonakalayo, ukukhethwa kwe-cap kanye ne-SEPA kanye namacala athile okusebenzisa ukuthola iziqukathi ezifanele. Ngokunaka kakhulu lezi zinto, abacwaningi bangaqinisekisa ukuthi izivivinyo zabo ze-Chromatographic zenziwa ngokunemba nokwethembeka, zikhiqiza imiphumela yelebhu ethembekile.

Hlola izinzuzo zokusebenzisa ama-headpace vials ku-chromatography ngalesi sihloko:Kungani ama-headspace vials asetshenziswa ku-chromatography? 12 engles

Okwedlule:

Intengo ethe xaxa kumanani e-chromatography vials wezindawo zocwaningo

Olandelayo:

Ama-Chromatogragraphy Vavials wokuhlolwa kwe-forensic toxicology