I-Forensic Toxicology iyingxenye ebalulekile yesayensi ye-forensic, edlala indima yenhlangano ekuphenyweni kobugebengu kanye nezinyathelo zomthetho. I-Foinicologists basebenzisa amasampula endalo afana negazi, umchamo kanye namasampula wezinwele ukuthola izidakamizwa, utshwala noma ezinye izinto ezinobuthi ezinobuthi abakhona. Ukuthola imiphumela eqondile nethembekile abaqasha i-GH Chromatography (GC) kanye ne-chromatography ewuketshezi (LC), kanye nama-vial akhethekile aklanywe ngokuqondile ukuhlolwa kwe-toxicology. Kulesi sihloko sihlola ukubaluleka kwazo njengezingxenye ezihlanganayo zokuhlaziywa kwe-toxicology.

I-Chromatography ku-forensic toxicology

I-Chromatography iyindlela ebalulekile esetshenziswa nge-toxicology ukuzehlukanisa futhi ikhombe izakhi ezahlukahlukene ezikhona ekuxubeni okuyinkimbinkimbi. Ngokusekelwe ezimisweni ezahlukahlukene zokuhlukaniswa, ezibandakanya ukuhlukanisa amasampula aphezulu phakathi kwesigaba sokuma kanye nesigaba seselula; Izakhi kusampula zizosebenzisana ngokuhlukile ngalezi zigaba eziholela ekwahlukanisweni okusekelwe ezinhlakeni zomzimba noma zamakhemikhali zezakhi ngazinye kusampula.

Amasu we-Chromatographic ku-forensic toxicology

Ama-Forensic Foticologists asebenzisa amasu ahlukahlukene we-Chromatography, kuya nge-Analytes abayihloliwe:

I-GEG CHROmatography (GC): I-Chromatography kaphethiloli isetshenziswa kabanzi ekuhlaziyeni kwezinto eziguqukayo ezifana notshwala, izidakamizwa, kanye namakhompiyutha ajwayelekile aphilayo. Ukuze usebenzise ngempumelelo i-GC, amasampula kufanele aqale ahlukaniswe nge-vaporid ngaphambi kokungeniswa kwikholomu yesigaba emile ukuze ahlukaniswe ngokusekelwe ekuhluphekeni kwengcindezi ye-vapor noma ngokuhambisana nesigaba sawo wesiteshi; Ama-vials akhethekile aklanyelwe ngqo le nqubo iqinisekisa umjovo wesampula oqondile ngokulahleka okuncane ngesikhathi senqubo.

I-Chromatography e-Liquid (LC): I-Chromatography ewuketshezi iyindlela yokuhlaziya eguquguqukayo ekwazi ukuyisingatha uhlu lwamakhompiyutha asuka ezintweni ezingezona ezonakala kanye nama-semivolatile. Ukwenza ukuhlaziya nale ndlela, isampula kufanele iqale ukuncibilika esigabeni se-liquid mobile ngaphambi kokudlula esigabeni sekholomu ngaphakathi kwekholomu. Izinhlobo ezahlukahlukene zamakholomu noma ama-vials angasetshenziswa ngokuya ngezidingo zokuhlukaniswa kwezihlaziyi kanye nezidingo zokuhlukanisa.

Ukuze kubhekwe ekujuleni kokujula ku-15 application ahlukahlukene wesigaba se-Chromatography Vavials, hlola le ndatshana efundisayo:Izicelo eziyi-15 zesimo se-Chromatography emikhakheni ehlukene

Ama-chromatography vials angamaqhawe angezinhle

Ama-chromatography vialsziyizingxenye ezihlanganayo ze-chromatography Systems, zivame ukunganakwa ngenxa yokugxila kumasu wokuhlaziya ngokwawo. Kodwa-ke lezi zinhlobo zidlala indima ebalulekile ekugcineni ukulondolozwa kwesampula ubuqotho kanye nokuhlaziywa kwengqondo:

Material and Ukuvumelana:

Ama-vials athathwa ngokujwayelekile ngengilazi ye-borossilte noma izinto zepulasitiki. Ingilazi ye-borosilate inthandwa kakhulu ngenxa yokumelana kwayo kwamakhemikhali aphezulu kanye nokuvuselelwa okuphansi; Zonke izinto zokwakha kumele zihambisane nazo zombili i-matrix yesampula kanye ne-chromatographic inqubo esetshenzisiwe ukugwema ukufakwa kwezinto ezingcolile.

Usayizi nevolumu:

Usayizi we-vial nevolumu kufanele kuhlangabezane nezidingo zesampula kanye nokucaciswa kwamathuluzi kwemiphumela efanelekile. Ama-vials amancane asetshenziselwa kangcono ama-Volumes alinganiselwe alinganiselwe ngenkathi ama-vials amakhulu kufanele asetshenziswe lapho kuphathwa amasampula e-bulkier. Ekugcineni, ukuncipha nokuvalwa kwezidingo kufanele kubhekwe.

Ukubopha nokuvalwa:

Izindlela ezifanele zokuvalwa kwezindawo nokuvalwa zibalulekile ukuze zigweme ukuhwamuka kwesampula nokungcola, ngama-screw cap, crimp cap noma ama-snap cap ngokuya ngethuluzi nokuthandwa kwelebhu.

Ukuhlanzeka nobumsulwa:

Ama-Vaials we-Chromatography kumele ahlanzwa kahle ukuze aqede noma yiziphi izinsalela noma ukungcola, ngenkathi amanye ama-vials ahlanzwe ngaphambili eqinisekisiwe njengokuhlangana namazinga athile okuhlanzeka. Ukulawulwa kwekhwalithi kanye nezinqubo zokuqinisekisa kumele futhi zibhekwe phakathi nenqubo yazo yokuhlanza.

Ukulawulwa kwekhwalithi nokuqinisekiswa

Ukunemba, ukunemba nokwethenjwa kubaluleke kakhulu kuma-lateratories we-forensic toxicology, anamathela ngokulawulwa kwekhwalithi okuqinile kanye namaphrothokholi wokuqinisekiswa ukuze aqinisekise imiphumela engaguquki futhi evumayo. Izifundo zokuqinisekiswa zibandakanya ukuhlola izinhlelo ze-Chromatography ezifana nama-vials ukuze kuhlangatshezwane nokunemba okucacisiwe, ukunemba, ukunemba kanye nenqubo yokuzwela; Ukulungiswa njalo kanye nokulinganisa kwale mishini - kufaka phakathi izinhlelo zokuphatha ze-vial - kubalulekile futhi ukufezekisa imiphumela engaguquki futhi evumelekile.

Ukugcina

Ama-chromatography vialsbangamathuluzi asemqoka ku-forensic toxicology, enikeza ukuhlukaniswa okunembile nokuhlaziywa kwamakhompiyutha ngaphakathi kwamasampula ayinkimbiko aphilayo. Lapho ukhetha lezi zinhlaka ukuze zisetshenziswe, ukucatshangelwa ngokucophelela kufanele zinikezwe iqhaza labo elisekususelwa kwisakhiwo sesampula kanye nezindlela ezikhethiwe ze-Chromatography. Ukulawulwa kwekhwalithi kanye nezinqubo zokuqinisekisa kuthuthukisa imiphumela yophenyo lwe-forensic, ukuhlinzeka ngesiqinisekiso esithe xaxa sokufuna ubulungiswa kanye nokusungula amaqiniso emacaleni ahilela izinto ezinobuthi. Intuthuko yezobuchwepheshe ifaka isandla kwi-Chromatography Vials 'ukuqhubeka kwethuthuka kanye nokwenziwa okusha, okwengeziwe okwandayo kokunemba nokusebenza kahle ngaphakathi kwama-alchicology Lab azungeze umhlaba wonke. Benza njengoba ompetha buthule beqiniseka ukuthi ubulungiswa busebenza ngokuqina kwesayensi nokunemba.

Ngokuqonda okuphelele kuma-HPLC Vavials, dlulise kule ndaba efundisayo:Imibuzo evame ukubuzwa kaningi ku-HPLC VIALS

Okwedlule:

I-18mm Chromatogragraphy Vials: Izici ezi-3 ezibucayi zokukhetha ezibucayi zokuhlaziywa kwengqondo

Olandelayo:

Konke odinga ukukwazi ngezinhlobo ezahlukene zezinto zokwenziwa ze-SEPTA yezinsimbi ze-Chromatography