Ukukhetha i-buffer efanelekile yesigaba sakho se-HPLC Mobile kubalulekile ekufezekiseni ukuhlukaniswa okuhle nokuhlaziywa kwakho amasampula. Ama-Buffers asiza ukugcina i-ph eqinile, ebalulekile ukulawula isimo se-Ionization of analytes yakho, ngaleyo ndlela ithinta isikhathi sayo sokugcina kanye nesimo esiphakeme. Ngezansi kungukubuka okuningiliziwe kokuthi ungakhetha kanjani i-buffer enhle kakhulu yohlelo lwakho lokusebenza lwe-HPLC.

Uyazi nge-HPLC Sampula Ukulungiswa kwesampula? Chofoza le ndatshana ukuze ufunde kabanzi: "Konke odinga ukukwazi nge-HPLC Sampula Ukulungiselela"

Ukubaluleka kokukhethwa kwe-buffer

I-buffer yisisombululo esikhona se-PH esingaguquki ngemuva kokungeza inani elincane le-acid noma isisekelo. Ngokusekelwe ekwakhiweni kwabo, ama-buffers angahlukaniswa njenge-acidic noma eyisisekelo. Ukusebenza kwe-buffer kunqunywa yi-PKA yayo, okuyinto efanelekile okufanele ibe ngaphakathi kwe- ± 1 PH unit wesigaba esithandekayo seselula PHP sokuthola amandla aphezulu.

Izici ezibalulekile okufanele ucabangele

I-1.ph Ibanga: I-PH yesigaba seselula kufanele okungenani ibe ngama-2 ph.Izikhungo ze-PH kude ne-PKA ye-Analyte. Lokhu kusiza ukucindezela i-ionization ye-acidic noma yokuhlaziya okuyisisekelo, ukukhulisa ukugcinwa kwazo kukholamu. Isibonelo, uma i-analyte iyi-acid ebuthakathaka ene-PKA ye-4.5, esebenzisa isigaba seselula nge-PH azungeze i-2.0 izoyigcina isesimweni esingewona ama-ionized, ukuthuthukisa ukugcinwa.

Uhlobo lwe-2.Buffer: Ama-Buffers ajwayelekile asetshenziswa ku-HPLC afaka:

I-Phosphate Buffers: Isebenza ngempumelelo kuhlu lwe-PH olungu-2.0 kuya ku-8.0, ama-phosphate buffers asetshenziswa kabanzi ngenxa yokuqina nokuhambisana kwawo okuhlaziya. Zilungele kakhulu izinhlelo ze-LC-UV.

I-Acetate Buffers: Lokhu kudumile futhi ngezindlela ze-LC-UV futhi zingasetshenziswa kuma-PH afanayo amabanga njenge-phosphate buffers.

Ama-Buffers aguquguqukayo: Okwezicelo ze-LC-MS, ama-buffers aguqukayo afana nokwakheka, i-acetate, ne-ammonium bicarbonate ayakhethwa ngoba angasuswa kalula ngesikhathi senqubo enkulu ye-mass spectrometry.

I-3.Bufferction Ukuhlushwa: Ukuhlushwa okujwayelekile kokuqala kwama-buffers ku-HPLC kuphakathi kuka-25-50 mm. Ukugxila okuphezulu kungathuthukisa ukwakheka okuphezulu kanye nokusebenza kahle kepha kungahle kuholele ekutheni kwenzeke lapho kuxutshwe ne-solvents ye-organic. Kungcono ukugcina ukugxila kwe-buffer ngaphansi kwama-25 mm ukugwema izinkinga.

Ubumsulwa be-4.creamical: Sebenzisa njalo ama-chromatographic-grade auffers avimbela ukungcoliswa futhi aqinisekise imiphumela ethembekile. Ukungcola kungaholela ekuxhumaneni okungalindelekile kanye nokuhluka ngezikhathi zokugcina.

Imiphumela yokushisa: Izinga lokushisa lingathonya i-PH ye-Buffer Solutions. Kubalulekile ukulungiselela nokugcina ama-buffers ekushiseni okungaguquki ukuze ulondoloze ukusebenza kwawo.

Ufuna ukwazi izimpendulo ezingama-50 mayelana ne-HPLC VIALS, sicela uhlole le ndatshana: Imibuzo evame ukubuzwa kaningi ku-HPLC VIALS

Izeluleko Ezisebenzayo zokulungiselela i-Buffer

Indlela Yokulungiselela: Ama-Buffers angalungiswa ngokuxubana amanani emali enobuningi bezakhi zabo ze-acidic nezamasisekelo noma ngokuthwebula isisombululo esiyisisekelo nge-acid. Gwema ukuguqula i-PH ngemuva kokungeza izixazululo ze-organic, ngoba lokhu kungaholela emiphumeleni engahambisani.

Izixazululo ezintsha: Lungiselela izixazululo ezintsha ze-Buffer njalo ukunciphisa ukukhula kwe-microbial kanye nokuwohloka kwezinto, ezingathinta ukusebenza kwe-chromatogagraph.

Ukugwema ukugqwala: ama-buffers athile, anjenge-citrate, angalungisa izingxenye zensimbi ezingagqwali ezinhlelweni ze-HPLC. Qinisekisa ukugcwala ngokuphelele kohlelo ngemuva kokusebenzisa ama-buffers anjalo ukuvimbela ukulimala.

Ukugcina

Ukukhetha i-buffer efanelekile yesigaba sakho se-HPLC Mobile kubalulekile ekufezekiseni imiphumela ethembekile nezenzekayo. Cabanga nge-PH, uhlobo lwe-Buffer, ukugxilisa ingqondo, nobumsulwa lapho ukhetha. Ngokulandela le mihlahlandlela, ungalungisa indlela yakho ye-HPLC ukuze uhlukanise ngempumelelo futhi uhlaziye amasampula akho.

Ufuna ukwazi intengo ye-HPLC VIALS, sicela uhlole le ndatshana: Intengo ye-HPLC VIALS: Imibuzo Ebuzwa Kakhulu Kakhulu

Okwedlule:

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