Ukuhlunga kokuhlunga kuyinkinga ebucayi ekuhlutjweni kwesirinji okungaholela ekungcolisweni kwesampula kanye nomonakalo wezinsimbi zokuhlaziya njenge-HPLC. Ukugwema ukuphumelela ukuhlunga, kubalulekile ukukhetha okulungileisihlungi sesirinjiFuthi ulandele izindlela ezinhle kakhulu ngesikhathi senqubo yokuhlunga. Nawa amanye amathiphu asemqoka:

Imibuzo ebuzwa njalo mayelana nokuhlunga ngezimpawu, qiniseka ukuthi ubheka le ndatshana efundisayo: Isihloko se- "Syringe Filter" Imibuzo Ebuzwa Njalo Kaningi

Khetha okokuhlunga okufanelekile nosayizi we-pore

Ukukhethwa kwezinto zokuhlunga kanye nosayizi we-pore kuncike kuhlobo lwesampula yakho:

Ngezixazululo zamanzi nama-solvents amaningi aphilayo, izihlungi ze-hydrophilic Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated Revenerated (RC

Gwema izihlungi eziqukethe ama-PFAS (PER- POLYFLUOROALLYL) ezingahle ziye kwisampula.

Cabanga ngezithako ezisebenzayo kwisampula yakho bese ukhetha impahla yokuhlunga ehambelana futhi engenamahhala.

Okwezicelo eziningi, usayizi we-pore pore 0.45 μm wanele ukususa izinhlayiya. Amasayizi amancane e-pore afana ne-0,2 μm angadingeka e-UHPLC noma asuse izinhlayiya ezinhle namagciwane.

Sebenzisa umfundzi wesampula ayinselele

Abaqanduli babeke phezulu kwe-Uptreaming Filter Confung kungasiza ukuvimbela ukuphumelela ngokunciphisa umthwalo we-microbial kanye nokususa izinhlayiya ezingadala ukuguquguquka kwengcindezi:

Izihlungi zokujula ezi-Adsorptive ziyizimangaliso ezisebenzayo zokuhlunga kwegciwane, ukususa ama-aggregates amancane angafaka isihlungi esiphansi.

Usebenzisa i-0.5 μm Prepilter evinjelwe ukuphumelela kwe-S. MarcescensIzihlungi ze-0.2 μmekuhlolweni okukodwa.

Kodwa-ke, ama-preilters angeze izindleko nobunzima, ngakho-ke ukusetshenziswa kwawo kufanele kulungiswe ngokususelwa kwisicelo esithile.

Uzosebenzisa kabusha lezi zihlungi zesirinji, uyazi yini ukuthi ingabe isihlungi se-syrinji esingasetshenziswa kabusha? Sicela ubheke le ndatshana: Okokuhlunga ngezimpawu, uzophinda usebenzise?

Lungiselela inqubo yokuhlunga

Isu elifanele ukhiye wokugwema ukuphumelela:

Amasampula wokuhlunga ngokushesha ngaphambi kokuhlaziywa ukuze anciphise ukukhula kwe-microbial.

Sebenzisa indlela yokuhlunga emnene njengokucindezela kunokuba i-vacuum ukugwema ukucheba amaseli.

Hlunga amavolumu anele ukuthola amazinga aphansi okungcoliswa - okungenani ama-100 ml esifundweni esisodwa. I-USP incoma ukuhlola i-10% yevolumu ye-batch.

Gwema ukugcina amasampula ahlungiwe ngezikhathi ezengeziwe ezingavumela ukukhula kwama-microbial.

Qinisekisa inqubo yokuhlunga

Ukuqinisekiswa kokuhlola kubalulekile ukuze kuqinisekiswe ukuthi inqubo yokuhlunga isebenza ngesicelo ngasinye esithile:

Inselelo yokuhlunga nge-matrix yangempela yesampula namagciwane afanele, hhayi izidalwa ezijwayelekile zokuhlola.

Amasiko abulawa yindlala angaba ngummeleli owengeziwe wezimo zangempela zomhlaba kunamasiko anothile acebile.

Linganisa yonke inqubo yokuhlunga, hhayi isihlungi uqobo uqobo.

Qinisekisa amazinga anele we-microbial inselelo kanye nama-sampula amavolumu wokuthola ukuphumelela.

Sebenzisa izihlungi zesirinji ezisezingeni eliphakeme

EnqumaIzihlungi zesirinjiKusuka kumenzi odumile kunganikeza ezinye isiqinisekiso esihlunga se-Filter ngeke kwenzeke:

Bheka izihlungi ezinezici ezifana nolwelwesi lokushisa-seal oluvimbela ukukhishwa kwezinhlayiyana.

Ikhanda elinele lekhanda ngaphakathi kwezindlu zokuhlunga ukuze kuhlaliswe amakhekhe ezinkulu zokuhlunga ngaphambi kokuxhuma.

Ukufanelekela ukusebenza kokuhlunga (isb., HPLC) kuqinisekisa ikhwalithi.

Ngokulandela lezi zindlela ezinhle kakhulu zokukhethwa kokuhlunga, ukusebenziseka kwenqubo, kanye nokuqinisekiswa kokuhlola, unganciphisa ubungozi bokuhlunga kokuhlunga futhi uqinisekise ubuqotho bamasampula akho kanye nemiphumela yokuhlaziya. Ukutshala imali ezihlungi ezisezingeni eliphakeme zesirinji kusuka kumkhiqizi othembekile nakho kuyisivikelo esibalulekile.

Ufuna ukwazi ulwazi oluphelele mayelana nesihlungi se-syrinji, sicela uhlole le ndatshana: Umhlahlandlela ophelele wokuhlunga kwesirinji: Izici, Ukukhetha, Intengo, kanye nokusebenzisa

Okwedlule:

Yikuphi i-buffer elungele isigaba sakho se-HPLC Mobile?

Olandelayo:

Yini umsebenzi wamashubhu wokuhlola ama-COD ekuhlaziyeni kwemvelo?